Biofilm formation of Achromobacter xylosoxidans on contact lens

Abstract

Achromobacter spp. may contaminate lenses, lens cases, and contact lens solutions and cause ocular infections. The aim of this study was to investigate the possibility of isolated strain of Achromobacter xylosoxidans to form biofilm on the surface of soft contact lenses (CL), to quantify the production of the formed biofilm, and compare it with the reference strains (Pseudomonas aeruginosa, Staphylococcus aureus, and Haemophilus influenzae). Bacterial strain isolated from one contact lens case was identified as A. xylosoxidans using Vitek2 Automated System. Biofilm forming capacity of isolated strain of A. xylosoxidans and reference strains of P. aeruginosa, S. aureus, and H. influenzae on soft CL were analyzed by commonly used microtitre plate method. Our results showed that isolated strain of A. xylosoxidans was capable to form biofilm on the surface of soft contact lens. A. xylosoxidans was strong biofilm producer while all examined reference strains were moderate biofilm producers. A. xylosoxidans appears to be superior biofilm producer on soft CL compared to reference strains.

Isolation and Antibiotic Resistance of Achromobacter xylosoxidans from Non-respiratory Tract Clinical Samples: A 10-year Retrospective Study in a Tertiary-care Hospital in Hungary

“…Similarly to other non-fermenting Gram-negative bacteria, Achromobacter species have a plethora of intrinsic resistance mechanisms: penicillins, 1-2 nd generation cephalosporins, chloramphenicol, macrolides and aminoglycosides [4][5][6][7][8][9][10][11][12][13]17,31,32]. Fluoroquinolones are usually considered as the part of a combination regimen (not as monotherapy) with carbapenems, therefore IMP, MER, TZP and SXT are the drugs of choice in these infections, preferably in combination.…”

Recent Advances in Anti-virulence Therapeutic Strategies With a Focus on Dismantling Bacterial Membrane Microdomains, Toxin Neutralization, Quorum-Sensing Interference and Biofilm Inhibition

“…This lifestyle can protect bacteria against potential environmental stress, such as antibiotics and host defense components (Hall and Mah, 2017; Tseng et al, 2018). In the clinical situation, biofilm formed by pathogenic bacteria can establish themselves on human surfaces or medical devices, including implants, catheter, endotracheal tubes and others (Rieger et al, 2016; Konstantinović et al, 2017; Kenaley et al, 2018; Silva et al, 2018). Biofilm formation on these surfaces can serve as a source of infection.…”

An Unusual Skin Infection With Achromobacter xylosoxidans

“…Achromobacter xylosoxidans has been documented to form biofilms on plastics, including on contact lenses, urinary and intravenous catheters, and reusable tissue dispensers treated with disinfectant solution. [4][5][6] One study demonstrated that A xylosoxidans is even capable of biodegradation of plastic, using the plastic as its sole source of carbon. 7 Our case illustrates an indolent infection with A xylosoxidans forming a granulomatous abscess at the site of an insulin pump that was left in place for 7 days in an immunocompetent patient.…”