Biofilm formation in uropathogenic Escherichia coli: association with adhesion factor genes

Zamani, Hojjatolah; Salehzadeh, Ali

doi:10.3906/sag-1707-3

Cited by 57 publications

(40 citation statements)

References 14 publications

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“…A study by Manuela et al reported that bio lm production was signi cantly associated with uoroquinolone resistance (30). In another similar study by Zamani et al it was found out that bio lm-producing UPEC were signi cantly associated with Fim gene (31).…”

Section: Discussionmentioning

confidence: 87%

Biofilm formation, antimicrobial susceptibility and virulence genes of Uropathogenic Escherichia coli isolated from clinical isolates in Uganda

Katongole

Nalubega

Florence

et al. 2020

Preprint

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Abstract Introduction: Uropathogenic E. coli is the leading cause of Urinary tract infections (UTIs), contributing to 80-90% of all community-acquired and 30-50% of all hospital-acquired UTIs. Biofilm forming Uropathogenic E. coli are associated with persistent and chronic inflammation leading to complicated and or recurrent UTIs. Biofilms provide an environment for poor antibiotic penetration and horizontal transfer of virulence genes which favors the development of Multidrug-resistant organisms (MDRO). Understanding biofilm formation and antimicrobial resistance determinants of Uropathogenic E. coli strains will provide insight into the development of treatment options for biofilm-associated UTIs. The aim of this study was to determine the biofilm forming capability, presence of virulence genes and antimicrobial susceptibility pattern of Uropathogenic E. coli isolates in Uganda. Methods: This was a cross-sectional study carried in the Clinical Microbiology and Molecular biology laboratories at the Department of Medical Microbiology, Makerere University College of Health Sciences. We randomly selected 200 Uropathogenic E. coli clinical isolates among the stored isolates collected between January 2018 and December 2018 that had significant bacteriuria (>105 CFU). All isolates were subjected to biofilm detection using the Congo Red Agar method and Antimicrobial susceptibility testing was performed using the Kirby disk diffusion method. The isolates were later subjected PCR for the detection of Urovirulence genes namely; Pap, Fim, Sfa, Afa, Hly and Cnf, using commercially designed primers.Results: In this study, 62.5% (125/200) were positive biofilm formers and 78% (156/200) of these were multi-drug resistant (MDR). The isolates were most resistant to Trimethoprim sulphamethoxazole and Amoxicillin (93%) followed by gentamycin (87%) and the least was imipenem (0.5%). Fim was the most prevalent Urovirulence gene (53.5%) followed by Pap (21%), Sfa (13%), Afa (8%), Cnf (5.5%) and Hyl (0%).Conclusions: We demonstrate a high prevalence of biofilm-forming Uropathogenic E. coli strains that are highly associated with the MDR phenotype. We recommend routine surveillance of antimicrobial resistance and biofilm formation to understand the antibiotics suitable in the management of biofilm-associated UTIs.

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Section: Discussionmentioning

confidence: 87%

Biofilm formation, antimicrobial susceptibility and virulence genes of Uropathogenic Escherichia coli isolated from clinical isolates in Uganda

Katongole

Nalubega

Florence

et al. 2020

Preprint

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“…These genes encoding for the type I pili, P ( pap ), F1C ( focA ), and S-fimbriae ( sfaS ) are considered to be important virulence factors for the induction of UTI and formation of biofilm [ 53 , 54 , 55 , 56 ]. A higher prevalence of sfaS (S-fimbriae) gene was observed for UPEC strains producing moderate to strong biofilms [ 57 ]. Similarly, Adamus-Bialek et al [ 55 ] and Naves et al [ 58 ] demonstrated that the virulence genes sfa , foc , and papG were more common in strong biofilm-producing E. coli isolates.…”

Section: Resultsmentioning

confidence: 99%

Inhibition and Inactivation of Uropathogenic Escherichia coli Biofilms on Urinary Catheters by Sodium Selenite

Narayanan

Nair

Muyyarikkandy

et al. 2018

IJMS

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Urinary tract infections (UTI) are the most common hospital-acquired infections in humans and are caused primarily by uropathogenic Escherichia coli (UPEC). Indwelling urinary catheters become encrusted with UPEC biofilms that are resistant to common antibiotics, resulting in chronic infections. Therefore, it is important to control UPEC biofilms on catheters to reduce the risk for UTIs. This study investigated the efficacy of selenium for inhibiting and inactivating UPEC biofilms on urinary catheters. Urinary catheters were inoculated with UPEC and treated with 0 and 35 mM selenium at 37 °C for 5 days for the biofilm inhibition assay. In addition, catheters with preformed UPEC biofilms were treated with 0, 45, 60, and 85 mM selenium and incubated at 37 °C. Biofilm-associated UPEC counts on catheters were enumerated on days 0, 1, 3, and 5 of incubation. Additionally, the effect of selenium on exopolysacchride (EPS) production and expression of UPEC biofilm-associated genes was evaluated. Selenium at 35 mM concentration was effective in preventing UPEC biofilm formation on catheters compared to controls (p < 0.05). Further, this inhibitory effect was associated with a reduction in EPS production and UPEC gene expression. Moreover, at higher concentrations, selenium was effective in inactivating preformed UPEC biofilms on catheters as early as day 3 of incubation. Results suggest that selenium could be potentially used in the control of UPEC biofilms on urinary catheters.

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“…E. coli can adhere to different surfaces, so they can easily form biofilms, which is an important feature of their virulence [28]. Different adhesion factors produced by E. coli may promote their adhesion and biofilm development, especially phenotypic expression of type 1 fimbriae, which was shown to be related to their biofilm formation ability [29]. Pseudomonas aeruginosa, a versatile organism that can adapt to a variety of environments, not only grows in aqueous environment but also can colonize and induce opportunistic infections [30].…”

Section: Discussionmentioning

confidence: 99%

Observations of Bacterial Biofilm on Ureteral Stent and Studies on the Distribution of Pathogenic Bacteria and Drug Resistance

et al. 2018

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Objective: This study aims to observe the morphological characteristics of bacterial biofilm on the surface of ureteral stents and analyze distribution characteristics of pathogens on the bacterial biofilm and drug resistance. Methods: Ureteral stents were sampled from 129 patients. A scanning electron microscope was used to observe the morphological characteristics of bacterial biofilms, and the Congo red medium was applied to screen bacterial biofilm strains on the renal pelvis section, ureter section, and bladder section respectively. Urine culture was performed, and the drug sensitive test analysis was carried out for the pathogenic bacteria detected. Results: Bacterial biofilms can be observed on the surface of ureteral stents, and these bacteria are embraced by large amounts of fiber membranes. A total of 107 patients were found to be positive for biofilms with a positive rate of 82.9%. The positive rates of the bladder section, ureter section, renal pelvis section, and urine culture were 85.0, 42.9, 67.3, and 24.3% respectively. Pathogenic bacteria mainly concentrated on Escherichia coli, and the drug resistance rate of the bacterial biofilm strains on the ureteral stent was relatively higher. Conclusion: The bacterial biofilm on the ureteral stent is an important factor that induces catheter-associated urinary tract infections.

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Biofilm formation in uropathogenic Escherichia coli: association with adhesion factor genes

Cited by 57 publications

References 14 publications

Biofilm formation, antimicrobial susceptibility and virulence genes of Uropathogenic Escherichia coli isolated from clinical isolates in Uganda

Biofilm formation, antimicrobial susceptibility and virulence genes of Uropathogenic Escherichia coli isolated from clinical isolates in Uganda

Inhibition and Inactivation of Uropathogenic Escherichia coli Biofilms on Urinary Catheters by Sodium Selenite

Observations of Bacterial Biofilm on Ureteral Stent and Studies on the Distribution of Pathogenic Bacteria and Drug Resistance

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