Biofilm formation in food processing plants and novel control strategies to combat resistant biofilms: the case of Salmonella spp.

Pang, Xinyi; Hu, Xin; Du, Xuewei; Lv, Chao; Yuk, Hyun‐Gyun

doi:10.1007/s10068-023-01349-3

Cited by 6 publications

(2 citation statements)

References 148 publications

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“…eDNA plays an important role in biofilm structural stability, contributing to bacterial aggregation and intercellular adhesion (Campoccia et al, 2021;Montanaro et al, 2011;Okshevsky & Meyer, 2015). Commercially, DNase I is an enzyme that could degrade eDNA and disrupt biofilm structure, considered a "green chemical" (Pang et al, 2023). Kim et al (2023) conducted a dose of 400 µg/mL DNase I treatments, which resulted in a decline of more than 1 log colony-forming unit (CFU)/cm 2 in Salmonella Enteritidis biofilms on stainless steel surfaces within 2 h. In the study of Nguyen and Burrows (2014) and Puga et al (2018), DNase I led to decreased attachment of L. monocytogenes and induced dispersion of preexisting biofilms.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of nisin‐loaded chitosan nanoparticles functionalized with DNase I for the removal of Listeria monocytogenes biofilms

Hu,

Du,

et al. 2024

Journal of Food Science

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Listeria monocytogenes biofilms represent a continuous source of contamination, leading to serious food safety concerns and economic losses. This study aims to develop novel nisin‐loaded chitosan nanoparticles (CSNPs) functionalized with DNase I and evaluate its antibiofilm activity against L. monocytogenes on food contact surfaces. Nisin‐loaded CSNPs (CS‐N) were first prepared by ionic cross‐linking, and DNase I was covalently grafted on the surface (DNase‐CS‐N). The NPs were subsequently characterized by Zetasizer Nano, transmission electron microscopy, Fourier transform infrared (FT‐IR), and X‐ray diffraction (XRD). The antibiofilm activity of NPs was evaluated against L. monocytogenes on polyurethane (PU). The DNase‐CS‐N was fabricated and characterized with quality attributes (particle size—427.0 ± 15.1 nm, polydispersity [PDI]—0.114 ± 0.034, zeta potential—+52.5 ± 0.2 mV, encapsulation efficiency—46.5% ± 3.6%, DNase conjugate rate—70.4% ± 0.2). FT‐IR and XRD verified the loading of nisin and binding of DNase I with chitosan. The DNase‐CS‐N caused a 3 log colony‐forming unit (CFU)/cm2 reduction of L. monocytogenes biofilm cells, significantly higher than those in CSNPs (1.4 log), CS‐N (1.8 log), and CS‐N in combination with DNase I (2.2 log) treatment groups. In conclusion, nisin‐loaded CSNPs functionalized with DNase I were successfully prepared and characterized with smooth surface and nearly spherical shape, high surface positive charge, and good stability, which is effective to eradicate L. monocytogenes biofilm cells on food contact surfaces, exhibiting great potential as antibiofilm agents in food industry.Practical ApplicationListeria monocytogenes biofilms are a common safety hazard in food processing. In this study, novel nanoparticles were successfully constructed and are expected to be a promising antibiofilm agent in the food industry.

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of nisin‐loaded chitosan nanoparticles functionalized with DNase I for the removal of Listeria monocytogenes biofilms

Hu,

Du,

et al. 2024

Journal of Food Science

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“…In this regard, a major challenge in eliminating bacterial biofilms is the need to remove the matrix and persistent cells. Common areas where Salmonella biofilms form in broiler farms include water systems (pipes, drinkers, tanks), feed lines and feeders, flooring and litter, and equipment and infrastructure ( Maes et al, 2019 ; Pang et al, 2023 ). These areas can harbor biofilms due to contamination with Salmonella , posing risks for both horizontal and vertical transmission.…”

Section: Introductionmentioning

confidence: 99%

Fighting Salmonella Infantis: bacteriophage-driven cleaning and disinfection strategies for broiler farms

Sevilla-Navarro,

Torres-Boncompte,

Garcia-Llorens

et al. 2024

Front. Microbiol.

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IntroductionSalmonella is a bacterium that can cause food-borne infections and is responsible for the most common gastrointestinal illnesses. The emergence of multi-drug resistant (MDR) strains worldwide is a major threat, representing a major challenge in public health. To reduce its incidence, the One Health approach is required, and the development of new biocontrol protocols will help prevent or eliminate the spread of Salmonella. Prevention measures, such as on-farm cleaning and disinfection protocols, are a crucial step in reducing infection to new flocks and eliminating bacteria that remain in the facilities. However, MDR Salmonella species, such as S. Infantis, are highly resistant to conventional cleaning and disinfection protocols, with an increased ability to persist in the broiler farm environment. The need for alternative biocontrol methods has led to the use of bacteriophages or phages, viruses that target bacteria, as promising tools. Thus, the aim of this study was to evaluate the efficacy of phages as a biocide against S. Infantis isolates in combination with cleaning and disinfection protocols in 10 commercial poultry farms.MethodsAll commercial farms selected in this study had persistent Salmonella, even after the routinely used cleaning and disinfection procedures. In addition, Salmonella isolated before treatment were phenotypically characterized by antimicrobial resistance patterns.ResultsThe results showed that 100% of S. Infantis were resistant to at least one antibiotic, and > 70% were MDR. Phages were then isolated against the in-farm bacteria, purified, and multiplied for each poultry farm. The cleaning and disinfection protocols included the application of the lytic phages (vB_Si_CECAV_FGS009; vB_Si_CECAV_FGS017; vB_Si_CECAV_FGS029 and vB_Si_CECAV _FGS030) twice at 24-h intervals between cleaning and disinfection. Following the cleaning and disinfection procedures, Salmonella detection was reduced from 100% after cleaning to 36% after applying the phages and dropped to 0% after the final step of disinfection, thus eliminating Salmonella from the farm facilities.DiscussionThis study demonstrates that bacteriophage application after cleaning and before disinfection enhances the removal of MDR Salmonella Infantis in commercial broiler farms, suggesting their use as biocontrol agents to reduce Salmonella, a major public health concern.

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A Review of Challenges and Solutions of Biofilm Formation of Escherichia coli: Conventional and Novel Methods of Prevention and Control

Sadeghzadeh,

Esfandiari,

Khaneghah

et al. 2024

Food Bioprocess Technol

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Biofilm formation in food processing plants and novel control strategies to combat resistant biofilms: the case of Salmonella spp.

Cited by 6 publications

References 148 publications

Preparation and characterization of nisin‐loaded chitosan nanoparticles functionalized with DNase I for the removal of Listeria monocytogenes biofilms

Preparation and characterization of nisin‐loaded chitosan nanoparticles functionalized with DNase I for the removal of Listeria monocytogenes biofilms

Fighting Salmonella Infantis: bacteriophage-driven cleaning and disinfection strategies for broiler farms

A Review of Challenges and Solutions of Biofilm Formation of Escherichia coli: Conventional and Novel Methods of Prevention and Control

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