Biofilm Formation, Epiphytic Fitness, and Canker Development in <i>Xanthomonas axonopodis</i> pv. <i>citri</i>

la, R M Donado de; Siciliano, Florencia; Enrique, Ramón; Sendín, Lorena Noelia; Filippone, Paula; Ps, Torres; Qüesta, Julia I.; Jm, Dow; Castagnaro, Atilio Pedro; Aa, Vojnov; Maraño, María Rosa

doi:10.1094/mpmi-20-10-1222

Cited by 237 publications

(289 citation statements)

References 69 publications

Supporting

Mentioning

251

Contrasting

Unclassified

Order By: Relevance

“…Epiphytic survival and biofilm formation have been suggested to be important for X. citri subsp. citri before it invades intercellular spaces (34). The monofunctional catalase KatE (40) and the rpf cell-to-cell quorum-sensing system, which is involved in exopolysaccharide, endoglucanase, and protease production (36), also contribute to the development of canker symptoms.…”

mentioning

confidence: 99%

The ColR/ColS Two-Component System Plays Multiple Roles in the Pathogenicity of the Citrus Canker Pathogen Xanthomonas citri subsp. citri

Yan

Wang

2011

J Bacteriol

View full text Add to dashboard Cite

Bacterial citrus canker disease, which is caused by Xanthomonas citri subsp. citri, is one of the most devastating diseases of citrus plants. In this study, we characterized the role of the two-component regulatory system ColR/ColS in the pathogenicity of X. citri subsp. citri. colS mutants (256A10 and 421E7), colR mutants (386C6 and 417E10), and a colR colS double mutant (306DSR) all lost pathogenicity and produced no symptoms on grapefruit leaves inoculated by either pressure infiltration or the spray method. The pathogenicity defect of the colS, colR, and colR colS mutants could be complemented using the wild-type colS, colR, and colR colS genes, respectively. Mutation of colS or colR significantly reduced X. citri subsp. citri growth in planta. The ColR/ColS system also played important roles in bacterial biofilm formation in glass tubes and on leaf surfaces, lipopolysaccharide (LPS) production, catalase activity, and tolerance of environmental stress, including phenol, copper, and hydrogen peroxide. Furthermore, quantitative reverse transcription-PCR assays demonstrated that the ColR/ColS system positively regulated the expression of important virulence genes, including hrpD6, hpaF, the O-antigen LPS synthesis gene rfbC, and the catalase gene katE. Overall, our data indicate that the two-component regulatory system ColR/ColS is critical for X. citri subsp. citri virulence, growth in planta, biofilm formation, catalase activity, LPS production, and resistance to environmental stress. Xanthomonas

show abstract

mentioning

confidence: 99%

The ColR/ColS Two-Component System Plays Multiple Roles in the Pathogenicity of the Citrus Canker Pathogen Xanthomonas citri subsp. citri

Yan

Wang

2011

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Thus, the possibility exists that, in Xcc, MopB regulates the expression of the gum operon via an unidentified mechanism, although phase variation may not necessarily be involved. The pathogenesis of black rot by Xcc involves multiple steps, including at least leaf attachment, epiphytic fitness, entry, colonization, biofilm formation/dispersal and bacterial spread to the vascular system (Chan & Goodwin, 1999;Dow et al, 2003;Rigano et al, 2007). The consequence of mutation in the Xcc mopB gene is pleiotropic, including formation of cell aggregates, reduction in xanthan production, adhesion and motility and increased sensitivity to stress conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The procedures used for the biotic adhesion assay were as described by Rigano et al (2007) with some modifications. Cabbage leaf discs of approximately 3.8 cm 2 were cut from healthy leaves and placed in a flask, to which 30 ml bacterial cells in XOLN medium containing 2 % (w/v) glucose at OD 550 of 0.1 was added, and incubated at 28 uC.…”

Section: +mentioning

confidence: 99%

See 1 more Smart Citation

Mutation of the gene encoding a major outer-membrane protein in Xanthomonas campestris pv. campestris causes pleiotropic effects, including loss of pathogenicity

Chen

Lin

et al. 2010

Microbiology

View full text Add to dashboard Cite

Xanthomonas cam pestris pv. campestris (Xcc) is the phytopathogen that causes black rot in crucifers. The xanthan polysaccharide and extracellular enzymes produced by this organism are virulence factors, the expression of which is upregulated by Clp (CRP-like protein) and DSF (diffusible signal factor), which is synthesized by RpfF. It is also known that biofilm formation/dispersal, regulated by the effect of controlled synthesis of DSF on cell-cell signalling, is required for virulence. Furthermore, a deficiency in DSF causes cell aggregation with concomitant production of a gum-like substance that can be dispersed by addition of DSF or digested by exogenous endo-beta-1,4-mannanase expressed by Xcc. In this study, Western blotting of proteins from a mopB mutant (XcMopB) showed Xcc MopB to be the major outer-membrane protein (OMP); Xcc MopB shared over 97% identity with homologues from other members of Xanthomonas. Similarly to the rpfF mutant, XcMopB formed aggregates with simultaneous production of a gummy substance, but these aggregates could not be dispersed by DSF or endo-beta-1,4-mannanase, indicating that different mechanisms were involved in aggregation. In addition, XcMopB showed surface deformation, altered OMP composition, impaired xanthan production, increased sensitivity to stressful conditions including SDS, elevated temperature and changes in pH, reduced adhesion and motility and defects in pathogenesis. The finding that the major OMP is required for pathogenicity is unprecedented in phytopathogenic bacteria

show abstract

“…Biofilm formation is important for the pathogenicity of Xcc and Xfa [94,95]. The genomes of Xfa and Xcc contain genes coding for adhesins predicted to be secreted by mechanisms resembling type V secretion systems (T5SS).…”

Section: Secretion Systems and Effectorsmentioning

confidence: 99%

Bacterial Citrus Diseases: Major Threats and Recent Progress

Jl¹

2017

JBMOA

View full text Add to dashboard Cite

Citriculture is an important, highly organized, and competitive sector of the Brazilian economy. Nonetheless, citrus production is constantly threatened by pathogens that cause considerable economic losses and severe social impacts. Among the major citrus diseases are citrus canker (CCK), citrus variegated chlorosis (CVC), and Huanglongbing (HLB), caused by members of the bacterial species Xanthomonas citri (Xcc), Xylella fastidiosa (Xfa), and 'Candidatus Liberibacter' (CaL), respectively. During the last years, management practices for CVC and HLB in Brazil have provided good results, maintaining the disease incidence at low levels. In contrast, CCK re-emerged as the main citrus disease because of the inefficacy of the current eradication program. In this review, we discuss about the biology of the plant-pathogen interactions, several controversial aspects on the taxonomy of the causal agents, the molecular mechanisms they use to cause disease in citrus plants, the strategies used for disease management and their limitations, and some emerging control alternatives that may be available for commercial production in the future, including transgenic and genome-edited plants with enhanced resistance.

show abstract

Biofilm Formation, Epiphytic Fitness, and Canker Development in Xanthomonas axonopodis pv. citri

Cited by 237 publications

References 69 publications

The ColR/ColS Two-Component System Plays Multiple Roles in the Pathogenicity of the Citrus Canker Pathogen Xanthomonas citri subsp. citri

The ColR/ColS Two-Component System Plays Multiple Roles in the Pathogenicity of the Citrus Canker Pathogen Xanthomonas citri subsp. citri

Mutation of the gene encoding a major outer-membrane protein in Xanthomonas campestris pv. campestris causes pleiotropic effects, including loss of pathogenicity

Bacterial Citrus Diseases: Major Threats and Recent Progress

Contact Info

Product

Resources

About