Biofilm formation by meat-borne Pseudomonas fluorescens on stainless steel and its resistance to disinfectants

Wang, Huhu; Cai, Liuyang; Li, Yunhan; Xu, Xinglian; Zhou, Guanghong

doi:10.1016/j.foodcont.2018.04.035

Cited by 82 publications

(32 citation statements)

References 35 publications

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“…Other treatments include ozone or electrolyzed water, successfully used for the removal of milk residues and biofilm-forming bacteria (such as P. fluorescens ) from stainless steel surfaces and in milk processing, including fluid milk, powdered milk products, and cheese [207,208]. The latter have been proven as effective alternative strategies for the elimination of P. fluorescens biofilm, which are also resistant to conventional sanitizers (e.g., sodium hypochlorite; [209]).…”

Section: Strategies To Control the Spread Of Antibiotic Resistant mentioning

confidence: 99%

Antibiotic Resistant Pseudomonas Spp. Spoilers in Fresh Dairy Products: An Underestimated Risk and the Control Strategies

Quintieri

Fanelli

Caputo

2019

Foods

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Microbial multidrug resistance (MDR) is a growing threat to public health mostly because it makes the fight against microorganisms that cause lethal infections ever less effective. Thus, the surveillance on MDR microorganisms has recently been strengthened, taking into account the control of antibiotic abuse as well as the mechanisms underlying the transfer of antibiotic genes (ARGs) among microbiota naturally occurring in the environment. Indeed, ARGs are not only confined to pathogenic bacteria, whose diffusion in the clinical field has aroused serious concerns, but are widespread in saprophytic bacterial communities such as those dominating the food industry. In particular, fresh dairy products can be considered a reservoir of Pseudomonas spp. resistome, potentially transmittable to consumers. Milk and fresh dairy cheeses products represent one of a few “hubs” where commensal or opportunistic pseudomonads frequently cohabit together with food microbiota and hazard pathogens even across their manufacturing processes. Pseudomonas spp., widely studied for food spoilage effects, are instead underestimated for their possible impact on human health. Recent evidences have highlighted that non-pathogenic pseudomonads strains (P. fluorescens, P. putida) are associated with some human diseases, but are still poorly considered in comparison to the pathogen P. aeruginosa. In addition, the presence of ARGs, that can be acquired and transmitted by horizontal genetic transfer, further increases their risk and the need to be deeper investigated. Therefore, this review, starting from the general aspects related to the physiological traits of these spoilage microorganisms from fresh dairy products, aims to shed light on the resistome of cheese-related pseudomonads and their genomic background, current methods and advances in the prediction tools for MDR detection based on genomic sequences, possible implications for human health, and the affordable strategies to counteract MDR spread.

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Section: Strategies To Control the Spread Of Antibiotic Resistant mentioning

confidence: 99%

Antibiotic Resistant Pseudomonas Spp. Spoilers in Fresh Dairy Products: An Underestimated Risk and the Control Strategies

Quintieri

Fanelli

Caputo

2019

Foods

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“…Hence, the effects of temperature on the efficacy of LED illumination against C. sakazakii biofilm should be further confirmed in future studies. An intact biofilm matrix is essential for biofilm to protect pathogen cells from death (Wang et al, 2018). In the current study, the integrity of the biofilm stereoscopic conformation of LED treatment groups was damaged compared with the control groups by observation of CLSM images (Figures 2A-F), and greater damage was observed at 25 • C which was consistent with the results of cells population determination (Figures 2A,D).…”

Section: Discussionmentioning

confidence: 99%

“…Illuminated and non-illuminated biofilms incubated at 25, 10, and 4 • C for 2 h were rinsed three times with 0.85% NaCl solution to remove loosely adhered thallus, and were then airdried at room temperature for later use, according to a method described by Wang et al (2018). The parameters of the ATR-FTIR spectrometer (NEXUS 670, Thermo Nicolet, USA) with 2 cm −1 resolution and 128 scans were set, and signals in the range of 2,000 to 800 cm −1 were captured.…”

Section: Attenuated Total Reflection Fourier-transformed Infrared Spementioning

confidence: 99%

Inactivation Efficacy of 405 nm LED Against Cronobacter sakazakii Biofilm

et al. 2020

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The objectives of this study were to evaluate the inactivation efficacy of a 405-nm light-emitting diode (LED) against Cronobacter sakazakii biofilm formed on stainless steel and to determine the sensitivity change of illuminated biofilm to food industrial disinfectants. The results showed that LED illumination significantly reduced the population of viable biofilm cells, showing reduction of 2.0 log (25°C), 2.5 log (10°C), and 2.0 log (4°C) between the non-illuminated and LED-illuminated groups at 4 h. Images of confocal laser scanning microscopy and scanning electron microscopy revealed the architectural damage to the biofilm caused by LED illumination, which involved destruction of the stereoscopic conformation of the biofilm. Moreover, the loss of biofilm components (mainly polysaccharide and protein) was revealed by attenuated total reflection Fourier-transformed infrared spectroscopy, and the downregulation of genes involved in C. sakazakii biofilm formation was confirmed by real time quantitative PCR analysis, with greatest difference observed in fliD. In addition, the sensitivity of illuminated-biofilm cells to disinfectant treatment was found to significantly increased, showing the greatest sensitivity change with 1.5 log reduction between non-LED and LED treatment biofilms in the CHX-treated group. These results indicated that 405 nm LED illumination was effective at inactivating C. sakazakii biofilm adhering to stainless steel. Therefore, the present study suggests the potential of 405 nm LED technology in controlling C. sakazakii biofilms in food processing and storage, minimizing the risk of contamination.

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“…In certain cases, bacterial biofilms can be beneficial and important in biotechnological applications (for production of bulk chemicals and enzymes) [9,10] and can be applied in agriculture (bioremediation and growth promotion) [11], wastewater treatment [12] and as an anticorrosive strategy [13]. However, given their increased tolerance to disinfectants (glutaraldehyde, sodium hypochlorite) [14], antimicrobial agents [15], metal ions and halogenated ions [16][17][18], biofilms [16,19] also often result in persistent infections and can be a source of several problems, like biofouling, pipeline clogging and product contamination [20]. For these reasons, biofilm control and detection are important.…”

Section: Introductionmentioning

confidence: 99%

Study on the Effect of Contrast Agent on Biofilms and Their Visualization in Porous Substrate Using X-ray μCT

et al. 2020

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Investigation of biofilms and visualization using non-destructive imaging techniques like X-ray μCT has recently gained interest. Biofilms are congregations of microorganisms that attach to surfaces and comprise of microbial cells embedded in extracellular polymeric substances (EPS). They are ubiquitous entities that are commonly found in any non-sterile setting and have direct implications on human health. Methods to visualize them in situ are highly needed to understand their behaviour (attachment and detachment) inside a substrate. Contrast-enhanced X-ray μCT is a 3D imaging technique that is capable of visualising objects that have very low attenuation contrast. The use of contrast agents in X-ray μCT has been an evolving process, however, the possible toxic effect of these chemical compounds against biofilms has not been studied in detail. In this study, we focus on the toxic effect of contrast agents and study the diffusion and drainage of contrast agents in biofilms. We propose using water-soluble potassium bromide (KBr) as a suitable contrast agent for enhancement of the attenuation coefficient of a monoculture of Pseudomonas fluorescens biofilms inside a porous substrate. At the given concentration, KBr proved to be less bactericidal compared to other commonly used contrast agents and at 5% w/v concentration we were able to clearly distinguish between the biofilm and the porous substrate.

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Biofilm formation by meat-borne Pseudomonas fluorescens on stainless steel and its resistance to disinfectants

Cited by 82 publications

References 35 publications

Antibiotic Resistant Pseudomonas Spp. Spoilers in Fresh Dairy Products: An Underestimated Risk and the Control Strategies

Antibiotic Resistant Pseudomonas Spp. Spoilers in Fresh Dairy Products: An Underestimated Risk and the Control Strategies

Inactivation Efficacy of 405 nm LED Against Cronobacter sakazakii Biofilm

Study on the Effect of Contrast Agent on Biofilms and Their Visualization in Porous Substrate Using X-ray μCT

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