Biofilm Formation by Lactic Acid Bacteria and Resistance to Environmental Stress

Kubota, Hiromi; Senda, Shouko; Nomura, Nobuhiko; Tokuda, Harukuni; Uchiyama, Hiroo

doi:10.1263/jbb.106.381

Cited by 228 publications

(148 citation statements)

References 23 publications

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“…It is suggested that not only the structure of biofilms but also individual cells in biofilms have an effect on the increased resistance toward the acidic environment. For L. plantarum JCM 1149, individual cells in biofilms alter gene expression profiles and regulation differently than planktonic bacteria (23,24). The enhanced resistance of selected biofilms to heavy metals also was studied extensively.…”

Section: Discussionmentioning

confidence: 99%

Real-Time Solvent Tolerance Analysis ofPseudomonassp. Strain VLB120ΔC Catalytic Biofilms

Halan

Schmid

Buehler

2011

Appl Environ Microbiol

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Biofilms are ubiquitous surface-associated microbial communities embedded in an extracellular polymeric (EPS) matrix, which gives the biofilm structural integrity and strength. It is often reported that biofilm-grown cells exhibit enhanced tolerance toward adverse environmental stress conditions, and thus there has been a growing interest in recent years to use biofilms for biotechnological applications. We present a time-and locus-resolved, noninvasive, quantitative approach to study biofilm development and its response to the toxic solvent styrene. Pseudomonas sp. strain VLB120⌬C-BT-gfp1 was grown in modified flow-cell reactors and exposed to the solvent styrene. Biofilmgrown cells displayed stable catalytic activity, producing (S)-styrene oxide continuously during the experimental period. The pillar-like structure and growth rate of the biofilm was not influenced by the presence of the solvent. However, the cells experience severe membrane damage during styrene treatment, although they obviously are able to adapt to the solvent, as the amount of permeabilized cells decreased from 75 to 80% down to 40% in 48 h. Concomitantly, the fraction of concanavalin A (ConA)-stainable EPS increased, substantiating the assumption that those polysaccharides play a major role in structural integrity and enhanced biofilm tolerance toward toxic environments. Compared to control experiments with planktonic grown cells, the Pseudomonas biofilm adapted much better to toxic concentrations of styrene, as nearly 65% of biofilm cells were not permeabilized (viable), compared to only 7% in analogous planktonic cultures. These findings underline the robustness of biofilms under stress conditions and its potential for fine chemical syntheses.

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Section: Discussionmentioning

confidence: 99%

Real-Time Solvent Tolerance Analysis ofPseudomonassp. Strain VLB120ΔC Catalytic Biofilms

Halan

Schmid

Buehler

2011

Appl Environ Microbiol

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“…The method of Kubota et al (25) adapted by Domínguez-Manzano et al (11) was used. Briefly, this method includes fixation of the biofilms with glutaraldehyde, followed by dehydration through graded ethanol steps and a final treatment in tert-butyl alcohol.…”

Section: Methodsmentioning

confidence: 99%

Formation of In Vitro Mixed-Species Biofilms by Lactobacillus pentosus and Yeasts Isolated from Spanish-Style Green Table Olive Fermentations

León-Romero

Domínguez-Manzano

Garrido‐Fernández

et al. 2016

Appl Environ Microbiol

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The present work details the in vitro interactions between Lactobacillus pentosus and yeast strains isolated from table olive processing to form mixed biofilms. Among the different pairs assayed, the strongest biofilms were obtained from L. pentosus and Candida boidinii strain cocultures. However, biofilm formation was inhibited in the presence of D-(؉)-mannose. In addition, biofilm formation by C. boidinii monoculture was stimulated in the absence of cell-cell contact with L. pentosus. Scanning electron microscopy revealed that a sort of "sticky" material formed by the yeasts contributed to substrate adherence. Hence, the data obtained in this work suggest that yeast-lactobacilli biofilms may be favored by the presence of a specific mate of yeast and L. pentosus, and that more than one mechanism might be implicated in the biofilm formation. This knowledge will help in the design of appropriate mixed starter cultures of L. pentosus-yeast species pairs that are able to improve the quality and safety of Spanish-style green table olive processing. U ntil recently, microbiologists had studied microorganisms as pure cultures of nonaggregated planktonic cells. However, it is now well accepted that in nature, microorganisms can exist within microbial communities named biofilms (1). Biofilms are defined as sessile microbial communities that are attached to a surface or each other, surrounded by a matrix of exopolysaccharide material (EPS) and extracellular DNA (eDNA) produced and released by the same microorganisms. According to Costerton et al. (2), the biofilm life cycle is part of a dominant survival strategy in natural environments.It is known that monocultural biofilms are rarely found in nature. Instead, they are composed of mixed species of bacteria and eukaryotes, which are thought to be more stable than monospecies films (3, 4). This behavior is also the case with microbial communities in food environments (5-10). In the particular case of olive table fermentations, the presence of polymicrobial communities composed of yeasts and lactic acid bacteria (LAB) attached to both biotic (skin of the olives) and abiotic (inner fermenter walls) surfaces has been reported (11-14). The predominant LAB adhered to the surface of this fruit belong mainly to the species Lactobacillus pentosus, although a variety of yeast species coexist in the same biofilms during fermentation. Candida boidinii, Debaryomyces etchelsii, Issatchenkia occidentalis, Pichia membranifaciens, Saccharomyces cerevisiae, and Wickerhamomyces anomalus are among the most prominent yeast species found in this fermented fruit (15).Apart from contributing to the sensory attributes of the final product and other advantageous activities, it has been recognized that yeasts have a beneficial role in the olive fermentation ecosystem by promoting the growth of L. pentosus through the production of essential B vitamins (15-18). In the last few years, various authors have described the biofilm formation process with mixed species of L. pentosus and yeasts in natura...

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“…2B, the sugar contents of the cells increased from the early EO phase to the AR phase, and finally decreased again in the AO phase. Since the sugar contents of cells have been found to be related to the polysaccharide attached to the cells, which could be detected only in the R strain, as described, 14,15) the culture appeared to be rich in cells having pellicle polysaccharides in the late EO phase to the AR phase. This might be related to the acetic acid resistance of the cells.…”

Section: Growth Behavior Of a Pasteurianus Ifo3283 In Ethanol Culturementioning

confidence: 89%

“…plantarum JCM1149 are more resistant to acetic acid and ethanol than planktonic cells. 14) Acetobacter species, Acetobacter tropicalis SKU1100 and Acetobacter aceti IFO3284, have been shown to produce capsular polysaccharides as a pellicle or biofilm, 15,16) and have two different types of cells, the R (rough colony) and S (smooth colony) strains, where the R strain but not the S strain produces the pellicle. 17,18) Thus, pellicle formation migh also be a defense mechanism for acetic acid resistance, though this has never been confirmed.…”

mentioning

confidence: 99%

Acetic Acid Fermentation ofAcetobacter pasteurianus: Relationship between Acetic Acid Resistance and Pellicle Polysaccharide Formation

Kanchanarach

Theeragool

Inoue

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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Biofilm Formation by Lactic Acid Bacteria and Resistance to Environmental Stress

Cited by 228 publications

References 23 publications

Real-Time Solvent Tolerance Analysis ofPseudomonassp. Strain VLB120ΔC Catalytic Biofilms

Real-Time Solvent Tolerance Analysis ofPseudomonassp. Strain VLB120ΔC Catalytic Biofilms

Formation of In Vitro Mixed-Species Biofilms by Lactobacillus pentosus and Yeasts Isolated from Spanish-Style Green Table Olive Fermentations

Acetic Acid Fermentation ofAcetobacter pasteurianus: Relationship between Acetic Acid Resistance and Pellicle Polysaccharide Formation

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