Biofilm formation by<i>Staphylococcus epidermidis</i>on peritoneal dialysis catheters and the effects of extracellular products from<i>Pseudomonas aeruginosa</i>

Pihl, Maria; Arvidsson, Anna; Skepö, Marie; Nilsson, Marie; Givskov, Michael; Tolker‐Nielsen, Tim; Svensäter, Gunnel; Davies, Julia R.

doi:10.1111/2049-632x.12035

Cited by 18 publications

(19 citation statements)

References 30 publications

(44 reference statements)

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“…It has been established that P. aeruginosa , in-vitro , negatively affects the attachment and growth of staphylococcal species, [43,50-52] something that could also be reflected in decreased biofilm production. These results are in keeping with the expected outcomes.…”

Section: Resultsmentioning

confidence: 99%

Collagen and hyaluronan at wound sites influence early polymicrobial biofilm adhesive events

2014

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BackgroundWounds can easily become chronically infected, leading to secondary health complications, which occur more frequently in individuals with diabetes, compromised immune systems, and those that have suffered severe burns. When wounds become chronically infected, biofilm producing microbes are often isolated from these sites. The presence of a biofilm at a wound site has significant negative impact on the treatment outcomes, as biofilms are characteristically recalcitrant to removal, in part due to the formation of a protective matrix that shield residents organisms from inimical forces. Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) are two of the organisms most prevalently isolated from wound sites, and are of particular concern due to their elevated levels of antibiotic resistance, rapid growth, and exotoxin production. In order to understand the biofilm forming abilities of these microbes in a simulated wound environment we used a microtiter plate assay to assess the ability of these two organisms to bind to proteins that are typically found at wound sites: collagen and hyaluronan.ResultsCollagen and hyaluronan were used to coat the wells of 96-well plates in collagen:hyaluronan ratios of 0:1, 3:1, 1:1, 1:3, and 1:0 . P. aeruginosa and MRSA were inoculated as mono- and co-cultures (1:1 and a 3:1 MRSA: P. aeruginosa). We determined that coating the wells with collagen and/or hyaluronan significantly increased the biofilm biomass of attached cells compared to an uncoated control, although no one coating formulation showed a significant increase compared to any other combination. We also noted that the fold-change increase for MRSA upon coating was greater than for P. aeruginosa.ConclusionsOur study suggests that the presence of collagen and/or hyaluronan at wound sites may be an important factor that influences the attachment and subsequent biofilm formation of notorious biofilm-formers, such as MRSA and P. aeruginosa. Understanding the kinetics of binding may aid in our comprehension of recalcitrant wound infection development, better enabling our ability to design therapies that would prevent or mitigate the negative outcomes associated with such infections.

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Section: Resultsmentioning

confidence: 99%

Collagen and hyaluronan at wound sites influence early polymicrobial biofilm adhesive events

2014

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“…Furthermore, rhamnolipids were shown to promote biofilm dispersal in many different microorganisms, including P. aeruginosa itself, although this effect is strain dependent (Rodrigues et al, 2006). For S. aureus and S. epidermidis , rhamnolipids were shown to induce biofilm dispersal and to inhibit adhesion in a dose-dependent manner (Rodrigues et al, 2006; Zezzi do Valle Gomes and Nitschke, 2012; Pihl et al, 2013; Figure 2, left upper panel).…”

Section: P Aeruginosa Produces a Wide Variety Of Molecules That Inhimentioning

confidence: 99%

“…Similar to S. aureus , the effect of P. aeruginosa on S. epidermidis is strain dependent as some P. aeruginosa strains have a more pronounced effect on some S. epidermidis strains while others are more resistant to P. aeruginosa (Pihl et al, 2010b). Nevertheless, extracellular products that prevent initial attachment of some S. epidermidis strains to surfaces might be an interesting option for the development of coatings for indwelling medical devices, like peritoneal dialysis catheters (Pihl et al, 2013). Moreover, in this model, P. aeruginosa supernatant components replaced serum proteins on the catheter surface and reduced S. epidermidis attachment (Pihl et al, 2013).…”

Section: P Aeruginosa and Other Staphylococcimentioning

confidence: 99%

“…Nevertheless, extracellular products that prevent initial attachment of some S. epidermidis strains to surfaces might be an interesting option for the development of coatings for indwelling medical devices, like peritoneal dialysis catheters (Pihl et al, 2013). Moreover, in this model, P. aeruginosa supernatant components replaced serum proteins on the catheter surface and reduced S. epidermidis attachment (Pihl et al, 2013). In addition, exposure of a S. epidermidis biofilm on a catheter to P. aeruginosa supernatant also caused dispersal of S. epidermidis (Pihl et al, 2013).…”

Section: P Aeruginosa and Other Staphylococcimentioning

confidence: 99%

“…Moreover, in this model, P. aeruginosa supernatant components replaced serum proteins on the catheter surface and reduced S. epidermidis attachment (Pihl et al, 2013). In addition, exposure of a S. epidermidis biofilm on a catheter to P. aeruginosa supernatant also caused dispersal of S. epidermidis (Pihl et al, 2013). The dispersed cells are, however, not killed making it less suitable as a treatment option and only interesting as a prevention strategy.…”

Section: P Aeruginosa and Other Staphylococcimentioning

confidence: 99%

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In vivo and In vitro Interactions between Pseudomonas aeruginosa and Staphylococcus spp.

Hotterbeekx

Kumar‐Singh

Goossens

et al. 2017

Front. Cell. Infect. Microbiol.

209

280

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The significance of polymicrobial infections is increasingly being recognized especially in a biofilm context wherein multiple bacterial species—including both potential pathogens and members of the commensal flora—communicate, cooperate, and compete with each other. Two important bacterial pathogens that have developed a complex network of evasion, counter-inhibition, and subjugation in their battle for space and nutrients are Pseudomonas aeruginosa and Staphylococcus aureus. Their strain- and environment-specific interactions, for instance in the cystic fibrosis lung or in wound infections, show severe competition that is generally linked to worse patient outcomes. For instance, the extracellular factors secreted by P. aeruginosa have been shown to subjugate S. aureus to persist as small colony variants (SCVs). On the other hand, data also exist where S. aureus inhibits biofilm formation by P. aeruginosa but also protects the pathogen by inhibiting its phagocytosis. Interestingly, such interspecies interactions differ between the planktonic and biofilm phenotype, with the extracellular matrix components of the latter likely being a key, and largely underexplored, influence. This review attempts to understand the complex relationship between P. aeruginosa and Staphylococcus spp., focusing on S. aureus, that not only is interesting from the bacterial evolution point of view, but also has important consequences for our understanding of the disease pathogenesis for better patient management.

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A Rapidly Responsive Sensor for Wireless Detection of Early and Mature Microbial Biofilms

et al. 2023

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Biofilm‐associated infections, which are able to resist antibiotics, pose a significant challenge in clinical treatments. Such infections have been linked to various medical conditions, including chronic wounds and implant‐associated infections, making them a major public‐health concern. Early‐detection of biofilm formation offers significant advantages in mitigating adverse effects caused by biofilms. In this work, we aim to explore the feasibility of employing a novel wireless sensor for tracking both early‐stage and matured‐biofilms formed by the medically relevant bacteria Staphylococcus aureus and Pseudomonas aeruginosa. The sensor utilizes electrochemical reduction of an AgCl layer bridging two silver legs made by inkjet‐printing, forming a part of near‐field‐communication tag antenna. The antenna is interfaced with a carbon cloth designed to promote the growth of microorganisms, thereby serving as an electron source for reduction of the resistive AgCl into a highly‐conductive Ag bridge. The AgCl‐Ag transformation significantly alters the impedance of the antenna, facilitating wireless identification of an endpoint caused by microbial growth. To the best of our knowledge, this study for the first time presents the evidence showcasing that electrons released through the actions of bacteria can be harnessed to convert AgCl to Ag, thus enabling the wireless, battery‐less, and chip‐less early‐detection of biofilm formation.

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Biofilm formation byStaphylococcus epidermidison peritoneal dialysis catheters and the effects of extracellular products fromPseudomonas aeruginosa

Cited by 18 publications

References 30 publications

Collagen and hyaluronan at wound sites influence early polymicrobial biofilm adhesive events

Collagen and hyaluronan at wound sites influence early polymicrobial biofilm adhesive events

In vivo and In vitro Interactions between Pseudomonas aeruginosa and Staphylococcus spp.

A Rapidly Responsive Sensor for Wireless Detection of Early and Mature Microbial Biofilms

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