Biofilm formation by ESBL-producing strains of Escherichia coli and Klebsiella pneumoniae

Surgers, Laure; Boyd, Anders; Girard, Pierre‐Marie; Arlet, Guillaume; Decré, Dominique

doi:10.1016/j.ijmm.2018.10.008

Cited by 45 publications

(42 citation statements)

References 41 publications

(43 reference statements)

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“…Accordingly, difference in ability to quickly adhere seems to exist within isolates of different bacterial species. The biofilm production displayed by our ESBLEC collection is also in accordance with that displayed by the ESBLEC collection recently published by Surgers et al (2018), i.e., predominance of weak biofilm producers among ESBLEC.…”

Section: Discussionsupporting

confidence: 89%

Association Between Kinetics of Early Biofilm Formation and Clonal Lineage in Escherichia coli

et al. 2019

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Background Escherichia coli biofilm formation has mostly been assessed in specific pathogenic E. coli groups. Here, we assessed the early biofilm formation (EBF), i.e., adhesion stage, using the BioFilm Ring Test ® on 394 E. coli clinical isolates (EC) [196 consecutively isolated (CEC) in 2016 and 198 ESBL-producing E. coli (ESBLEC) isolated in 2015]. Then, biofilm-forming ability was contrasted with phylogroups, clonotypes ( fumC - fimH ), and sequence types (STs), all being used to define clones, virulence factors (VF), and FimB. Result According to both biofilm production levels at 2, 3, and 5 h, and EBF kinetics over 5 h, CEC and ESBLEC isolates segregated into three EBF groups: strong (G1), moderate (G2), and weak (G3) producers. At 2 h, strong producers were more frequent among CEC ( n = 28; 14.3%) than among ESBLEC ( n = 8; 4%) ( P = 0.0004). As CEC and ESBLEC isolates showed similar individual EBF kinetics in each group, a comparison of isolate features between each group was applied to gathered CEC and ESBLEC isolates after 2 h of incubation, 2 h being the most representative time point of the CEC and ESBLEC isolate segregation into the three groups. Phylogroup B2 displayed by 51.3% of the 394 isolates was more frequent in G1 (77.8%) than in G3 (47.6%) ( P = 0.0006). The 394 isolates displayed 153 clones, of which 31 included at least three isolates. B2-CH14-2-ST127, B2-CH40-22-ST131, B2-CH52-5/14-ST141, and E-CH100-96-ST362 clones were associated with G1 ( P < 0.03) and accounted for 41.7% of G1 isolates. B2-CH40-30-ST131 clone was associated with G3 ( P < 0.0001) and accounted for 25.5% of G3 isolates. VF mean was higher among G1 than among G3 isolates ( P < 0.001). FimB-P2 variant was associated with G1 ( P = 0.0011) and FimB-P1 variant was associated with G3 ( P = 0.0023). Clone, some VF, and FimB were associated with EBF, with clonal lineage being able to explain 72% of the variability of EBF. Conclusion Among our 394 isolates, <10% are able to quickly and persistently produce high biofilm levels over 5 h. These isolates belong to a few clones previously described in various studies as dominant gut colonizers in mammalians and birds and comprised the B2-CH40-22-ST131 clone, i.e., the ancestor of the globally disseminated B2-CH40-30-ST131 clone that is the dominant clone among the weak biofilm producers.

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Section: Discussionsupporting

confidence: 89%

Association Between Kinetics of Early Biofilm Formation and Clonal Lineage in Escherichia coli

et al. 2019

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“…Seriously, Klebsiella pneumoniae-caused diseases and other secondary pathogenic bacteria infections eventually result in the death of goats [4]. Thus, an efficient, sensitive, and robust Klebsiella pneumoniae antibody detection method is highly desired to guide the prevention, intervention and control of the spread of Klebsiella pneumoniae in goat-farming industry [5].…”

Section: Introductionmentioning

confidence: 99%

Establishment and evaluation of an indirect ELISA for detection of antibodies to goat Klebsiella pneumonia

et al. 2021

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Background Klebsiella pneumonia, a Gram-negative bacterium belonging to the genus Enterobacter, causes many human and livestock diseases. Notably, infected goats may develop pneumonia, septicemia, which can lead to occasional death, resulting in great economic losses in goat-farming industry. However, there are little systematic methods for detection of goat Klebsiella pneumoniae in livestock production. Results In this study, we developed a Klebsiella pneumoniae goat polyclonal antibody and established an indirect ELISA method to detect the Klebsiella pneumoniae. After screening and optimizing the conditions for detection, we determined the optimal working dilutions of the coated-bacterial antigen, the polyclonal antibody, and the enzyme-labeled secondary antibody that were 1:800 (2.99 × 107 CFU/ml), 1:6400, and 1:5000, respectively. The optimal condition of coating and blocking were both 4 °C for 12 h. The optimal dilution buffers of bacterial antigen, the antibodies, and the blocking buffer were 0.05 mol/L carbonate buffer, 1% BSA phosphate buffer, and 1.5% BSA carbonate buffer, respectively. The cut-off value was determined to be 0.28, and the analytical sensitivity was 1:800 (dilution of a positive sample). Furthermore, there was no cross-reaction between the coated antigen and goat serum positive for antibodies against other bacteria, indicating that indirect ELISA could detect Klebsiella pneumoniae specifically in most cases. The average coefficients of variation of intra-assay and inter-assay were 4.37 and 5.17% indicating favorable reproducibility of indirect ELISA. In the detection of clinical veterinary samples, the positive rate of indirect ELISA was 6.74%, higher than that of conventional agglutination assays. Conclusions Taken together, we successfully established an indirect ELISA method for detecting antibodies against Klebsiella pneumoniae in goats, which can be applied in production.

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“…Sevirously, Klebsiella pneumoniae-caused diseases along with other various pathogenic bacteria infections eventually result in the death of goats [4]. Thus, an e cient, sensitive, and robust Klebsiella pneumoniae detection method is highly desired to guide the prevention, intervention and control of the spread of Klebsiella pneumoniae in goat-farming industry [5].…”

Section: Introductionmentioning

confidence: 99%

Establishment and Evaluation of an Indirect ELISA for Detection of Antibodies to Goat Klebsiella Pneumonia

Chen¹,

Shang

Niu

et al. 2020

Preprint

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Background: Klebsiella pneumonia, a Gram-negative bacterium belonging to the genus Enterobacter, causes many human and livestock diseases. Notably, infected goats may develop pneumonia, septicemia, which can lead to occasional death, resulting in great economic losses in goat-farming industry. However, there are no systematic methods for detection of Klebsiella pneumoniae in goats currently. Results: In this study, we developed a Klebsiella pneumoniae goat polyclonal antibody and established an indirect ELISA method to detect the Klebsiella pneumoniae. After screening and optimizing the conditions for detection, we determined the optimal working dilutions of the coated-bacterial antigen, the polyclonal antibody, and the enzyme-labeled secondary antibody that were 1:800 (2.99x107 CFU/ml), 1:6400, and 1:5000, respectively. The optimal condition of coating and blocking were both 4 °C for 12 h. The optimal dilution buffers of bacterial antigen, the antibodies, and the blocking buffer were 0.05 mol/L carbonate buffer, 1% BSA phosphate buffer, and 1.5% BSA carbonate buffer, respectively. The cut-off value was determined to be 0.28, and the analytical sensitivity was 1:800. Furthermore, there was no cross-reaction between the coated antigen and goat serum positive for other common bacteria, indicating that indirect ELISA could detect Klebsiella pneumoniae specifically in most cases. The average coefficients of variation of intra-assay and inter-assay were 4.37% and 5.17% indicating favorable reproducibility of indirect ELISA. In the detection of clinical veterinary samples, the positive rate of indirect ELISA was 6.74%, higher than that of conventional agglutination assays. Conclusions: Taken together, our results suggested that the method that we established has promising applications in production, laying the foundation for epidemiology, serological investigation and clinical veterinary diagnosis of Klebsiella pneumoniae in goats.

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Biofilm formation by ESBL-producing strains of Escherichia coli and Klebsiella pneumoniae

Cited by 45 publications

References 41 publications

Association Between Kinetics of Early Biofilm Formation and Clonal Lineage in Escherichia coli

Association Between Kinetics of Early Biofilm Formation and Clonal Lineage in Escherichia coli

Establishment and evaluation of an indirect ELISA for detection of antibodies to goat Klebsiella pneumonia

Establishment and Evaluation of an Indirect ELISA for Detection of Antibodies to Goat Klebsiella Pneumonia

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