Biofilm formation by Candida albicans on various prosthetic materials and its fluconazole sensitivity: a kinetic study

Abstract

Candida albicans has the ability to colonize various materials used in prostheses. In this report, we have studied the kinetics of biofilm formation on prosthetic materials and their susceptibility to fluconazole at various stages of development. Results indicated that C. albicans efficiently adheres to and colonizes polystyrene, polyvinylchloride, silicon, and polycarbonate surfaces. Candida albicans biofilm formation was observed to be both strainand substrate dependent. Adhesion of cells to solid substrates was found sufficient to induce fluconazole resistance. Drug susceptibility at different stages of biofilm growth showed that Candida biofilms on these substrates are highly resistant to fluconazole. The study focuses on the limitations of fluconazole to combat biofilm-related infections and emphasizes the need for better therapeutic strategies against prosthesis-associated C. albicans infections.

Sensitization of Candida albicans biofilms to various antifungal drugs by cyclosporine A

“…While, to analyze the effects on mature biofilms, medium with a range of drug concentrations was added to the 24 hour old biofilms. The plates were further incubated for 48 hours at 37 °C [23]. Density of the cells survived in biofilm forms was analyzed through metabolic activity in XTT-formazan reduction assay.…”

“…2, 3-bis (2-methoxy-4-nitro-sulfophenyl)-2H-tetrazolium-5-carboxanilide] (Sigma-Aldrich, India) reduction assay [23]. Briefly, XTT solution was prepared by mixing 1 mg/ml XTT salt in PBS and stored at −20 °C.…”

Sensitization of Candida albicans biofilms to fluconazole by terpenoids of plant origin

“…Sensitization of Candida albicans biofilms to fluconazole by terpenoids of plant origin (Received February 19, 2014;Accepted June 19, 2014) Sonali DST-FIST and UGC-SAP School of Life Sciences, SRTM University, Nanded, 431606 (MS), India prescribed drug fluconazole (Shinde et al, 2012). Additionally, biofilms may act as reservoirs of infectious cells to cause re-infections (LaFleur et al, 2006).…”

In vitro Resistance Pattern of Selected Antifungal Azoles against Candida albicans Biofilms on Silicone Nasogastric Tube

“…Moreover, imaging of the biofilms using fluorescence microscopy revealed a heterogeneous structure, a network of extracellular matrix strands, characterized by the presence of yeast cells and hyphal forms. These Fungal infections, device-related biofilm infections caused by C. albicans in particular, had been linked with an increase use of medical device among immunocompromised patients or for surgical advances (Shinde et al, 2012). In 2015, it was reported that these caused 65% of infections in catheterized patients (Yousif et al, 2015).…”