Biofilm formation and multidrug resistance in nosocomial isolates of Acinetobacter

Amin, Muzafar; Pai, Vidya; Qayoom, Sumaira; Arshi, Syed; Khurshid, Syed

doi:10.18231/2394-5478.2018.0088

Cited by 3 publications

(5 citation statements)

References 10 publications

(13 reference statements)

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“…The results of this study correlates with various other studies done by Uppe et al, Rewdiwala et al, Kaur et al, Sharma et al, [13,14,16,19] A. baumannii, K.pneumoniae and P. aeruginosa showed multiple drug resistance which in concordance with various other studies done by Baidya et al, Sharma et al, Amin et al, Tomar et al and Natham et al [7,19,20,21,22] . In the present study, B. cepacia isolates showed susceptibility towards levofloxacin, minocycline, meropenem and cotrimoxazole.…”

Section: Resultssupporting

confidence: 92%

Antibiotic Resistance and Biofilm Formation in Gram Negative Bacteria Isolated From Endotracheal Tubes in Intensive Care Unit of a Tertiary Care Hospital

Kaur,

Nazir Reshi

2024

IJMRA

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Biofilm formation has been found to play a central role in many human infections in which antibiotic resistance is common. Bacteria can colonize and form biofilms on almost any surface, including natural and synthetic surfaces the present study was conducted with an aim to detect biofilm formation in ET tubes/ ET secretions received from ICU patients by microtiter plate method. In the present study a total of 85 isolates were obtained out of 400 samples. Among 85 isolates of gram- negative bacteria, maximum number of isolates were of K. pneumoniae (41.17%) followed by A. baumannii (40%), P. aeruginosa (10.5%), E. coli (5.88%) and B.cepacia (2.35%). All isolates were tested for antimicrobial sensitivity/resistance by Vitek 2 Compact system. All the isolates were further tested for biofilm production by microtiter plate method and optical density (OD value) was measured. Biofilm production was distributed by strong, medium, and weak. It is concluded that stringent hygiene protocols, routine tube changes and the use of antimicrobial coatings are crucial for prevention of biofilm development in ICU settings.

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Section: Resultssupporting

confidence: 92%

Antibiotic Resistance and Biofilm Formation in Gram Negative Bacteria Isolated From Endotracheal Tubes in Intensive Care Unit of a Tertiary Care Hospital

Kaur,

Nazir Reshi

2024

IJMRA

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“…In particular, Abidi et al showed that biofilm production was higher among MDR P. aeruginosa strains than in non-MDR strains [ 6 ]. Moreover, Amin et al found that the A. baumannii MDR strains produced more biofilm than the non-MDR ones [ 7 ]. The association between the MDR phenotype and biofilm production underlined the need to better investigate the resistance profiles of biofilm producing strains.…”

Section: Discussionmentioning

confidence: 99%

“…Abidi et al showed that biofilm production was higher among MDR Pseudomonas aeruginosa ( P. aeruginosa ) strains than non-MDR strains [ 6 ]. Moreover, Qayoom et al found that the Acinetobacter baumannii ( A. baumannii ) MDR produced more biofilm than the non-MDR ones [ 7 ]. Regarding Staphylococcus aureus ( S. aureus ), Manandhar et al associated biofilm production with Methicillin resistance [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Gram-positive and Gram-negative bacteria and fungi contribute to the emergence of DRIs. The most frequently encountered strains include coagulase-negative staphylococci (CoNS), Enterococcus faecalis ( E. faecalis ), Klebsiella pneumoniae ( K. pneumoniae ), A. baumannii , P. aeruginosa , and Candida albicans [ 7 ]. Among these, S. aureus and Staphylococcus epidermidis ( S. epidermidis ) are estimated to cause approximately 50–70% of catheter biofilm infections [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Antibiotic Resistance and Biofilm Production among Clinical Strain Isolated from Medical Devices

Folliero

Franci

Dell’Annunziata

et al. 2021

International Journal of Microbiology

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Microbial biofilms pose a serious threat to patients requiring medical devices (MDs). Prolonged periods of implantation carry a high risk of device-related infections (DRIs). Patients with DRIs often have negative outcomes following the failure of antibiotic treatment. Resistant DRIs are mainly due to the MDs contamination by bacteria producing biofilm. The present study aimed to detect biofilm formation among MD bacterial isolates and to explore their antibiotic resistance profile. The study was conducted on 76 MDs, collected at University Hospital of Campania “Luigi Vanvitelli,” between October 2019 and September 2020. Identification of isolates and antibiotic susceptibility testing were performed using Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and Phoenix Becton Dickinson, respectively. Biofilm-forming abilities were assessed using the tissue culture plate (TCP) method. Among the 94 MDs isolated strains, 42.7% were Gram-positive, 40.3% Gram-negative, and 17% Candida species. Among 78 bacterial strains, 43.6% were non-biofilm producers while 56.4% produced biofilms. All biofilm producing isolates were sensitive to a limited spectrum of antibiotic classes. All moderate and strong biofilm producers and 81% of weak biofilm producers were Multidrug Resistance (MDR) strains. In contrast, among non-biofilm producers, only 11.8% were classified as MDR strains. Our results highlighted that Sulfamides and Glycopeptides for the major Gram-positive strains and Fluoroquinolones, Carbapenems, and Aminoglycosides for the most represented Gram-negative isolates could be the most suitable therapeutic choice for most biofilm-DRIs.

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“…(100%) (p<0.05) being the most frequent. Meanwhile, Abidi et al [35]and Amin et al [36] reported a higher incidence of MDR-biofilm formation in P. aeruginosa and the ACB complex, respectively. Considering the existence of a positive correlation between the prevalence of MDR-and XDR-phenotypes among biofilm formations, it would be worthwhile to further investigate the resistance profiles associated with biofilm-producing strains.…”

Section: Plos Onementioning

confidence: 99%

Assessment of four in vitro phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates

Basnet,

Tamang,

Shrestha

et al. 2023

PLoS ONE

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Introduction The lack of standardized methods for detecting biofilms continues to pose a challenge to microbiological diagnostics since biofilm-mediated infections induce persistent and recurrent infections in humans that often defy treatment with common antibiotics. This study aimed to evaluate diagnostic parameters of four in vitro phenotypic biofilm detection assays in relation to antimicrobial resistance in aerobic clinical bacterial isolates. Methods In this cross-sectional study, bacterial strains from clinical samples were isolated and identified following the standard microbiological guidelines. The antibiotic resistance profile was assessed through the Kirby-Bauer disc diffusion method. Biofilm formation was detected by gold standard tissue culture plate method (TCPM), tube method (TM), Congo red agar (CRA), and modified Congo red agar (MCRA). Statistical analyses were performed using SPSS version 17.0, with a significant association considered at p<0.05. Result Among the total isolates (n = 226), TCPM detected 140 (61.95%) biofilm producers, with CoNS (9/9) (p<0.001) as the predominant biofilm former. When compared to TCPM, TM (n = 119) (p<0.001) showed 90.8% sensitivity and 70.1% specificity, CRA (n = 88) (p = 0.123) showed 68.2% sensitivity and 42% specificity, and MCRA (n = 86) (p = 0.442) showed 65.1% sensitivity and 40% specificity. Juxtaposed to CRA, colonies formed on MCRA developed more intense black pigmentation from 24 to 96 hours. There were 77 multi-drug-resistant (MDR)-biofilm formers and 39 extensively drug-resistant (XDR)-biofilm formers, with 100% resistance to ampicillin and ceftazidime, respectively. Conclusion It is suggested that TM be used for biofilm detection, after TCPM. Unlike MCRA, black pigmentation in colonies formed on CRA declined with time. MDR- and XDR-biofilm formers were frequent among the clinical isolates.

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Biofilm formation and multidrug resistance in nosocomial isolates of Acinetobacter

Cited by 3 publications

References 10 publications

Antibiotic Resistance and Biofilm Formation in Gram Negative Bacteria Isolated From Endotracheal Tubes in Intensive Care Unit of a Tertiary Care Hospital

Antibiotic Resistance and Biofilm Formation in Gram Negative Bacteria Isolated From Endotracheal Tubes in Intensive Care Unit of a Tertiary Care Hospital

Evaluation of Antibiotic Resistance and Biofilm Production among Clinical Strain Isolated from Medical Devices

Assessment of four in vitro phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates

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