Biofilm Formation Ability of Listeria monocytogenes Isolates from Raw Ready-to-Eat Seafood

Abstract

Listeria monocytogenes is of great concern as a foodborne pathogen. Many ready-to-eat foods are widely contaminated with this organism and have caused listeriosis outbreaks and sporadic cases in many countries. In Japan, there is a high incidence of L. monocytogenes contamination, specifically in raw ready-to-eat seafood. Identical L. monocytogenes subtypes have been isolated repeatedly from samples of food manufactured at a given store or processing plant, and researchers suspected that certain L. monocytogenes isolates have formed biofilms at these sites. A microtiter plate biofilm formation assay was conducted, and all raw ready-to-eat seafood isolates tested were able to form biofilms to various degrees. Biofilm formation by L. monocytogenes isolates of lineage I was significantly greater (P = 0.000) than that by isolates of lineage II. However, isolates of clonal lineages formed different levels of biofilms, indicating that the ability to form a biofilm is affected positively or negatively by environmental factors.

Exploring the Diversity of Listeria monocytogenes Biofilm Architecture by High-Throughput Confocal Laser Scanning Microscopy and the Predominance of the Honeycomb-Like Morphotype

“…Previous work showed variable capacities of the strains to form biofilms depending on their lineage (28). Some works showed that lineage I strains produced more biofilms than lineage II strains (15,16), and others reached the opposite conclusion (17,19). In our study, a weak correlation was detected between the strain lineage and the structure of the biofilms.…”

“…Indeed, most published reports focusing on the biofilm formation of several strains are based on global quantitative measurements (15)(16)(17)(18)(19).…”

comK Prophage Junction Fragments as Markers for Listeria monocytogenes Genotypes Unique to Individual Meat and Poultry Processing Plants and a Model for Rapid Niche-Specific Adaptation, Biofilm Formation, and Persistence

“…This has resulted in numerous costly recalls, disease cases, and outbreaks, which are often associated with high mortality (94). As a result, a zero-tolerance policy for L. monocytogenes currently exists for RTE meat and poultry products manufactured in the United States.Specific resident strains of L. monocytogenes have been found to persist for months to more than a decade (70,78,95,105) in various types of food processing plants manufacturing meat products (5, 38, 40, 90, 105), poultry products (4-6, 32, 78, 85), dairy products (4,5,37,48,53,101), seafood products (4,5,73,86,95), vegetables (5), and sandwiches (8) and also in retail environments (88). These persistent strains are genetically distinct from transient strains that are isolated sporadically (4, 5).…”

Análise da expressão gênica, formação de biofilmes e adesão/invasão a célula Caco-2 por Listeria monocytogenes em diferentes condições encontradas no trato gastrintestinal, em alimentos e em presença de bacteriocinas

“…Entretanto, diferentes estudos têm quantificado os biofilmes em placa de microtitulação baseando-se na quantificação de biomassa, de viabilidade e da matriz dos biofilmes (CHANG et al, 2012;HARVEY;KEENAN;GILMOUR, 2007;PEETERS, NELIS, COENYE;2008;SHAKERI et al, 2007, TAKAHASHI et al, 2009.…”

“…O ensaio com placa de microtitulação possui vantagens em relação a outros métodos para avaliar a formação de biofilmes em termos de objetividade, precisão, simplicidade e rapidez para, por exemplo, avaliar as diferenças na formação de biofilmes entre cepas ou determinar a eficácia de biocidas (CHANG et al, 2012;HARVEY;KEENAN;GILMOUR, 2007;PEETERS, NELIS, COENYE;2008;SHAKERI et al, 2007;TAKAHASHI et al, 2009;TRESSE et al, 2007).…”