Biofilm establishment, biofilm persister cell formation, and relative gene expression analysis of type II toxin-antitoxin system in Klebsiella pneumoniae

Narimisa, Negar; Amraei, Fatemeh; Kalani, Behrooz Sadeghi; Azarnezhad, Asaad; Jazi, Faramarz Masjedian

doi:10.1016/j.genrep.2020.100846

Cited by 17 publications

(12 citation statements)

References 30 publications

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“…The MBEC values of Thy-NPs were higher than their MBIC values. These results could be due to the presence of persistent cells with slow or absent growth within biofilms that are also not destroyed by Thy-NPs treatment [ 34 ]. Another contributing factor may be the overexpression of the efflux pump by sessile biofilm-forming cells [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm Synergistic Activity of Streptomycin in Combination with Thymol-Loaded Poly (Lactic-co-glycolic Acid) Nanoparticles against Klebsiella pneumoniae Isolates

Bisso

Kuaté

Boulens

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Thymol is an important component of essential oils found in the oil of thyme, is extracted mainly from Thymus vulgaris, and was shown to act synergistically with streptomycin against Klebsiella pneumoniae biofilms. Additionally, thymol could be encapsulated into poly (lactic-co-glycolic acid) (PLGA) nanoparticles to overcome issues related to its low water solubility and high volatility. The present study aimed to investigate the antibiofilm activity of thymol-loaded PLGA nanoparticles (Thy-NPs) alone and in combination with streptomycin against biofilms of K. pneumoniae isolates. Methods. The broth microdilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The antibiofilm activities were determined by the safranin dye assay. The synergistic effect of Thy-NPs with streptomycin was assessed by the checkerboard method. The kinetic study of the biofilm biomass and time-kill assay were further performed. Results. Thy-NPs exhibited the highest antibacterial activity against K. pneumoniae isolates, with MIC values ranging from 1 to 8 µg/mL. Additionally, Thy-NPs showed the highest antibiofilm activity against K. pneumoniae isolates with minimal biofilm inhibitory concentration (MBIC) and minimal biofilm eradication concentration (MBEC) values ranging from 16 to 64 µg/mL and from 32 to 128 µg/Ml, respectively. The combination treatment combining Thy-NPs with streptomycin showed a synergistic effect against the inhibition of biofilm formation and eradication of biofilms of K. pneumoniae isolates with fractional inhibitory concentration index values ranging from 0.13 to 0.28. In addition, the MBIC and MBEC values of streptomycin against K. pneumoniae isolates were dramatically reduced (up to 128-fold) in combination with Thy-NPs, suggesting that Thy-NPs would enhance the antibiofilm activity of streptomycin. The biomass and time-kill kinetics analysis confirmed the observed synergistic interactions and showed the bactericidal activity of streptomycin in combination with Thy-NPs. Conclusions. Our results indicate that the synergistic bactericidal effect between streptomycin and Thy-NPs could be a promising approach in the control of biofilm-associated infections caused by K. pneumoniae.

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Section: Discussionmentioning

confidence: 99%

Antibiofilm Synergistic Activity of Streptomycin in Combination with Thymol-Loaded Poly (Lactic-co-glycolic Acid) Nanoparticles against Klebsiella pneumoniae Isolates

Bisso

Kuaté

Boulens

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…The results of this study showed that the TA system function's involved in the formation of persister cells of biofilm depends on the growth stage of the strains, the type of antibiotic, and strain. Narimisa et al evaluated the expression of type TA systems ( relE1/relB1, relE2/relB2, hipA/hipB, vapB/vapC, phd/doc and mazF/mazE) in the persister cell formation of biofilm which except for phd/doc system, all level of TA systems expression were increased [31]. Chenglong Sun et al observed that the MqsR/MqsA system is involved in the formation of persister cells of biofilm Pseudomonas putida KT2440 [32].…”

Section: Discussionmentioning

confidence: 99%

Biofilm Persister Cell Formation, and Relative Gene Expression Analysis of Type II Toxin-Antitoxin System in Pseudomonas Aeruginosa Strains in the Exponential and Stationary Phases

Zadeh

Kalani

Ari

et al. 2021

Preprint

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Chronic and persistent infections and therapy failure are concerning issues in patients with Pseudomonas aeruginosa infections. Presence of persister cells in biofilm considers as one from the causes for antibiotic resistance and treatment failure. Consequently, in this research, the expression of TA type II systems into persister formation of biofilm was assessed in presence of colistin ciprofloxacin antibiotics into exponential and stationary phases. The results showed that TA systems at different stages of bacterial growth in biofilm have different functions that subsequently, cause differences in the presence amount of persister cells at each stage of bacterial growth. The expression of the systems were measured by Real-Time PCR method. Keywords: Persister cell; Biofilm; Pseudomonas aeruginosa; TA systems; Real-time PCR; Exponential and Stationary phases

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“…the Vapc family VapB mutations were selected after successive ampicillin treatments, and vapBC upregulation has been observed in K. pneumoniae upon antibiotic challenges [8,23,24]. Notably, vapBC is the most abundant TA module in M. tuberculosis, and ΔvapC12 shows reduced cholesterol-mediated persistence required for survival inside macrophages [54].…”

Section: Pemikmentioning

confidence: 99%

Bacterial survivors: evaluating the mechanisms of antibiotic persistence

Shi¹,

Zarkan

2022

Microbiology

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Bacteria withstand antibiotic onslaughts by employing a variety of strategies, one of which is persistence. Persistence occurs in a bacterial population where a subpopulation of cells (persisters) survives antibiotic treatment and can regrow in a drug-free environment. Persisters may cause the recalcitrance of infectious diseases and can be a stepping stone to antibiotic resistance, so understanding persistence mechanisms is critical for therapeutic applications. However, current understanding of persistence is pervaded by paradoxes that stymie research progress, and many aspects of this cellular state remain elusive. In this review, we summarize the putative persister mechanisms, including toxin–antitoxin modules, quorum sensing, indole signalling and epigenetics, as well as the reasons behind the inconsistent body of evidence. We highlight present limitations in the field and underscore a clinical context that is frequently neglected, in the hope of supporting future researchers in examining clinically important persister mechanisms.

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Biofilm establishment, biofilm persister cell formation, and relative gene expression analysis of type II toxin-antitoxin system in Klebsiella pneumoniae

Cited by 17 publications

References 30 publications

Antibiofilm Synergistic Activity of Streptomycin in Combination with Thymol-Loaded Poly (Lactic-co-glycolic Acid) Nanoparticles against Klebsiella pneumoniae Isolates

Antibiofilm Synergistic Activity of Streptomycin in Combination with Thymol-Loaded Poly (Lactic-co-glycolic Acid) Nanoparticles against Klebsiella pneumoniae Isolates

Biofilm Persister Cell Formation, and Relative Gene Expression Analysis of Type II Toxin-Antitoxin System in Pseudomonas Aeruginosa Strains in the Exponential and Stationary Phases

Bacterial survivors: evaluating the mechanisms of antibiotic persistence

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