Biofilm Electrochemistry

and, Haluk Beyenal; Babauta, Jerome T.

doi:10.1002/9781119097426.ch5

Cited by 6 publications

(4 citation statements)

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“…Microbial three-electrode electrochemical cells (M3Cs) were used to test the current generating properties of biocomposites by measuring chronoamperometry (CA) at a specified applied electrode potential (E CA ) against a reference electrode. [30][31][32][33] In S. oneidensis MR-1, the characteristic redox potentials of flavinbased mediated electron transfer (MET, −0.4 V to −0.3 V versus Ag/AgCl) and membrane-protein direct electron transfer (DET, 0 V versus Ag/AgCl) to electrodes are well established. [30,[34][35][36][37] Because CPE-K (M n = 6638 g mol −1 ) is expected to form a network by labile noncovalent interactions, a dialysis membrane (3.5 kD MWCO) was used to confine the polymer and the cells inside a well above the gold working electrode (area = 2 cm 2 , depth = 247 µm ± 5).…”

Section: Doi: 101002/adma201908178mentioning

confidence: 99%

Living Bioelectrochemical Composites

McCuskey

Leifert

et al. 2020

Advanced Materials

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Composites, in which two or more material elements are combined to provide properties unattainable by single components, have a historical record dating to ancient times. Few include a living microbial community as a key design element. A logical basis for enabling bioelectronic composites stems from the phenomenon that certain microorganisms transfer electrons to external surfaces, such as an electrode. A bioelectronic composite that allows cells to be addressed beyond the confines of an electrode surface can impact bioelectrochemical technologies, including microbial fuel cells for power production and bioelectrosynthesis platforms where microbes produce desired chemicals. It is shown that the conjugated polyelectrolyte CPE‐K functions as a conductive matrix to electronically connect a three‐dimensional network of Shewanella oneidensis MR‐1 to a gold electrode, thereby increasing biocurrent ≈150‐fold over control biofilms. These biocomposites spontaneously assemble from solution into an intricate arrangement of cells within a conductive polymer matrix. While increased biocurrent is due to more cells in communication with the electrode, the current extracted per cell is also enhanced, indicating efficient long‐range electron transport. Further, the biocomposites show almost an order‐of‐magnitude lower charge transfer resistance than CPE‐K alone, supporting the idea that the electroactive bacteria and the conjugated polyelectrolyte work synergistically toward an effective bioelectronic composite.

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Section: Doi: 101002/adma201908178mentioning

confidence: 99%

Living Bioelectrochemical Composites

McCuskey

Leifert

et al. 2020

Advanced Materials

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“…The idea behind doing the abiotic EIS was to calculate the Ohmic resistance in the absence and presence of the membrane. Subtracting the ohmic resistance of the blank MFC without the membrane from the MFCs with the membrane is used to calculate the Ohmic resistance of the respective membrane 56 . Least Ohmic resistance was observed in the SMN membrane (0.8 Ω) which is one‐eighth of the Ohmic resistance of the soil (4.7 Ω).…”

Section: Resultsmentioning

confidence: 99%

Modification of clayware ceramic membrane for enhancing the performance of microbial fuel cell

Suransh

Tiwari

Mungray

2020

Env Prog and Sustain Energy

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The aim of this study was to develop an economically viable clayware ceramic membrane that exhibits proton mass transfer comparable to the commercially available membrane (Nafion 117) for microbial fuel cell (MFC). The clayware ceramic membrane made from red soil was modified using cation exchangers like montmorillonite (MMT) and vermiculite (VC), and by spray coating of MMT composite with Nafion solution. Nafion-117 (a commercial membrane) and membrane prepared using only red soil were used as a standard and control, respectively. Other membranes include 20% blend of MMT with red soil (SM); 20% VC with red soil (SV); 10% blend of each MMT and VC with red soil (SMV); and SM membrane spray-coated with Nafion solution (SMN). The addition of cation exchangers enhances the performance of the clayware ceramic membranes as compared to the control, and coating of Nafion solution on SMN leads it to perform even better. Average open circuit voltage and average operating voltage for the SMN membranes were 670 ± 17.63 mV and 82 ± 5.69 mV, respectively, which are the best among all the fabricated membranes. The power density of the SMN membrane was 84.3 mW/m 3 which is five times that of the control. The study demonstrates that SMN membrane can be used as an alternate for more costly polymeric membranes in MFC.

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“…2a) performed on the anode brushes at day 52 and 100 235 yielded broad and poorly defined electrochemical signals. The interpretation of such voltammograms may be complicated by a high uncompensated resistance between working electrode and reference electrode (Babauta and Beyenal, 2015a). While an oxidation peak can be clearly identified at potentials near where the anode was held, the peak current did not increase with an increase of scan rate (SI Fig.…”

Section: Encouraging the Growth Of Cable Bacteria On Poised Electrodesmentioning

confidence: 99%

Inducing the Attachment of Cable Bacteria on Oxidizing Electrodes

Li¹,

Reimers²,

Alleau³

2019

Preprint

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The scope of the present study is to introduce electrochemical reactors as a tool for investigating the growth of novel filamentous cable bacteria and their unique extracellular electron transfer ability. New evidence that cable bacteria are widely distributed in sediments throughout an estuarine system connected to the NE Pacific Ocean is also presented. Cable bacteria found within Yaquina Bay, Oregon, USA, appear to cluster with the genus, Candidatus Electrothrix. Results of a 135-day bioelectrochemical reactor experiment confirm a previous observation that cable 10 bacteria can grow on oxidatively poised electrodes suspended in anaerobic seawater above reducing sediments.However, several diverse morphologies of Desulfobulbaceae filaments, cells, and colonies were observed on the carbon fibers of the suspended electrodes including encrusted chains of cells. These observations provide new information to suggest what conditions will induce cable bacteria to perform electron donation to an electrode surface, further informing future experiments to culture cable bacteria apart from a sediment matrix. 15 IntroductionLong distance electron transfer (LDET) is a mechanism used by certain microorganisms to generate energy through the transfer of electrons over distances greater than a cell-length. These microorganisms may pass electrons across dissolved redox shuttles, nanofiber-like cell appendages, outer-membrane cytochromes, and/or mineral nanoparticles to connect extracellular electron donors and acceptors Lovley, 2016). Recently, a novel type of LDET 20 exhibited by filamentous bacteria in the family of Desulfobulbaceae was discovered in the uppermost centimeters of various aquatic, but mainly marine, sediments (Malkin et al., 2014;Pfeffer et al., 2012). These filamentous bacteria, also known as "cable bacteria", electrically connect two spatially separated redox half-reactions and generate electrical current over distances that can extend to centimeters, which is an order of magnitude longer than previously recognized LDET distances (Meysman, 2017). 25The unique ability of cable bacteria to perform LDET creates a spatial separation of oxygen reduction in oxic surface layers of sediment from sulfide oxidation in subsurface layers (Meysman, 2017). The spatial separation of these two half-reactions also creates localized porewater pH extremes in oxic and sulfidic layers, which induces a series of secondary reactions that stimulate the geochemical cycling of elements such as iron, manganese, calcium, phosphorus, and nitrogen (Kessler et al., 2018;Rao et al., 2016; Seitaj et al., 2015;Sulu-Gambari et al., 2016b, 2016a. In addition 30 to altering established perceptions of sedimentary biogeochemical cycling and microbial ecology (Meysman, 2017;Nielsen and Risgaard-Petersen, 2015), cable bacteria also possess intriguing structural features that may inspire new engineering applications in areas of bioenergy harvesting and biomaterial design (Lovley, 2016). Pfeffer et al., 2012). Therefore to initiate this enquiry, sedimen...

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Biofilm Electrochemistry

Cited by 6 publications

References 73 publications

Living Bioelectrochemical Composites

Living Bioelectrochemical Composites

Modification of clayware ceramic membrane for enhancing the performance of microbial fuel cell

Inducing the Attachment of Cable Bacteria on Oxidizing Electrodes

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