Biofilm Development

Tolker‐Nielsen, Tim

doi:10.1128/microbiolspec.mb-0001-2014

Cited by 178 publications

(126 citation statements)

References 93 publications

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“…strain MR-S7 (Kusada et al, 2014). The importance of QS for the biofilm formation could be due to secretion of important compounds like extracellular DNA, the biosurfactant rhamnolipid and the secretion of the BapA-protein as shown in P. aeruginosa (Tolker-Nielsen, 2015). Furthermore, QS-compounds play an important role in P. fluorescence 2p24 for its colonization on wheat roots and development of biocontrol ability toward the take-all disease fungus (Wei and Zhang, 2006).…”

Section: Discussionmentioning

confidence: 99%

Systemic Responses of Barley to the 3-hydroxy-decanoyl-homoserine Lactone Producing Plant Beneficial Endophyte Acidovorax radicis N35

Han

Trost

et al. 2016

Front. Plant Sci.

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Quorum sensing auto-inducers of the N-acyl homoserine lactone (AHL) type produced by Gram-negative bacteria have different effects on plants including stimulation on root growth and/or priming or acquirement of systemic resistance in plants. In this communication the influence of AHL production of the plant growth promoting endophytic rhizosphere bacterium Acidovorax radicis N35 on barley seedlings was investigated. A. radicis N35 produces 3-hydroxy-C10-homoserine lactone (3-OH-C10-HSL) as the major AHL compound. To study the influence of this QS autoinducer on the interaction with barley, the araI-biosynthesis gene was deleted. The comparison of inoculation effects of the A. radicis N35 wild type and the araI mutant resulted in remarkable differences. While the N35 wild type colonized plant roots effectively in microcolonies, the araI mutant occurred at the root surface as single cells. Furthermore, in a mixed inoculum the wild type was much more prevalent in colonization than the araI mutant documenting that the araI mutation affected root colonization. Nevertheless, a significant plant growth promoting effect could be shown after inoculation of barley with the wild type and the araI mutant in soil after 2 months cultivation. While A. radicis N35 wild type showed only a very weak induction of early defense responses in plant RNA expression analysis, the araI mutant caused increased expression of flavonoid biosynthesis genes. This was corroborated by the accumulation of several flavonoid compounds such as saponarin and lutonarin in leaves of root inoculated barley seedlings. Thus, although the exact role of the flavonoids in this plant response is not clear yet, it can be concluded, that the synthesis of AHLs by A. radicis has implications on the perception by the host plant barley and thereby contributes to the establishment and function of the bacteria-plant interaction.

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Section: Discussionmentioning

confidence: 99%

Systemic Responses of Barley to the 3-hydroxy-decanoyl-homoserine Lactone Producing Plant Beneficial Endophyte Acidovorax radicis N35

Han

Trost

et al. 2016

Front. Plant Sci.

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“…1). For example, there are a variety of bacterial structures such as adhesins, type IV pili, and lipopolysaccharide (LPS) that are involved in attachment, and these bacterial structures are specifically regulated by environmental cues [9,34]. Recent studies demonstrated that the initiation of biofilm formation occurs with an increase in c-di-GMP, an intracellular second messenger [35][36][37][38][39].…”

Section: Attachment Of P Aeruginosa Biofilmsmentioning

confidence: 99%

Pseudomonas aeruginosa Biofilm, a Programmed Bacterial Life for Fitness

Lee

Yoon²

2017

Journal of Microbiology and Biotechnology

161

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A biofilm is a community of microbes that typically inhabit on surfaces and are encased in an extracellular matrix. Biofilms display very dissimilar characteristics to their planktonic counterparts. Biofilms are ubiquitous in the environment and influence our lives tremendously in both positive and negative ways. Pseudomonas aeruginosa is a bacterium known to produce robust biofilms. P. aeruginosa biofilms cause severe problems in immunocompromised patients, including those with cystic fibrosis or wound infection. Moreover, the unique biofilm properties further complicate the eradication of the biofilm infection, leading to the development of chronic infections. In this review, we discuss the history of biofilm research and general characteristics of bacterial biofilms. Then, distinct features pertaining to each stage of P. aeruginosa biofilm development are highlighted. Furthermore, infections caused by biofilms on their own or in association with other bacterial species (i.e., multispecies biofilms) are discussed in detail.

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“…In natural environments, microorganisms form complex multicellular surface-associated communities known as biofilms. The first step of biofilm formation is the reversible attachment of single cells to the surface (Goller and Romeo, 2008;Tolker-Nielsen, 2015;Berne et al, 2018). For most bacteria, the initial attachment step requires flagellar motility and pili.…”

Section: Introductionmentioning

confidence: 99%

Feedback regulation of Caulobacter crescentus holdfast synthesis by flagellum assembly via the holdfast inhibitor HfiA

Berne

Ellison

Agarwal

et al. 2018

Molecular Microbiology

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To permanently attach to surfaces, Caulobacter crescentusproduces a strong adhesive, the holdfast. The timing of holdfast synthesis is developmentally regulated by cell cycle cues. When C. crescentusis grown in a complex medium, holdfast synthesis can also be stimulated by surface sensing, in which swarmer cells rapidly synthesize holdfast in direct response to surface contact. In contrast to growth in complex medium, here we show that when cells are grown in a defined medium, surface contact does not trigger holdfast synthesis. Moreover, we show that in a defined medium, flagellum synthesis and regulation of holdfast production are linked. In these conditions, mutants lacking a flagellum attach to surfaces over time more efficiently than either wild-type strains or strains harboring a paralyzed flagellum. Enhanced adhesion in mutants lacking flagellar components is due to premature holdfast synthesis during the cell cycle and is regulated by the holdfast synthesis inhibitor HfiA. hfiA transcription is reduced in flagellar mutants and this reduction is modulated by the diguanylate cyclase developmental regulator PleD. We also show that, in contrast to previous predictions, flagella are not necessarily required for C. crescentus surface sensing in the absence of flow, and that arrest of flagellar rotation does not stimulate holdfast synthesis. Rather, our data support a model in which flagellum assembly feeds back to control holdfast synthesis via HfiA expression in a c-di-GMP-dependent manner under defined nutrient conditions.

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Biofilm Development

Cited by 178 publications

References 93 publications

Systemic Responses of Barley to the 3-hydroxy-decanoyl-homoserine Lactone Producing Plant Beneficial Endophyte Acidovorax radicis N35

Systemic Responses of Barley to the 3-hydroxy-decanoyl-homoserine Lactone Producing Plant Beneficial Endophyte Acidovorax radicis N35

Pseudomonas aeruginosa Biofilm, a Programmed Bacterial Life for Fitness

Feedback regulation of Caulobacter crescentus holdfast synthesis by flagellum assembly via the holdfast inhibitor HfiA

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