Biofilm, City of Microbes

Watnick, Paula I.; Kolter, Roberto

doi:10.1128/jb.182.10.2675-2679.2000

Cited by 1,262 publications

(870 citation statements)

References 53 publications

Supporting

Mentioning

819

Contrasting

Unclassified

Order By: Relevance

“…The acylhomoserine B. ADAM, G. S. BAILLIE AND L. J. DOUGLAS lactone type of signalling or quorum-sensing molecule appears to be confined to gram-negative bacteria and, not unexpectedly, attempts to identify such compounds in single species C. albicans biofilms have been unsuccessful (R. A. Caddick and L. J. Douglas, unpublished results). To date, little information is available on the role of intercellular signalling in mixed species biofilms although it may differ significantly from that observed in single species biofilms [4]. It could be particularly important where competition for attachment to the surface is fierce, and is likely to be a critical factor in the diversity and distribution of micro-organisms within a mixed species biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…Biofilm cells are organised into structured communities enclosed within a matrix of extracellular material. They are phenotypically different from planktonic or suspended cells; notably, they resist host defences and display a significantly decreased susceptibility to antimicrobial agents [3,4]. It is likely that multiple mechanisms are involved in drug resistance.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mixed species biofilms of Candida albicans and Staphylococcus epidermidis

Adam

Baillie

Douglas

2002

Journal of Medical Microbiology

316

223

View full text Add to dashboard Cite

A simple catheter disk model system was used to study the development in vitro of mixed species biofilms of Candida albicans and Staphylococcus epidermidis, two organisms commonly found in catheter-associated infections. Two strains of S. epidermidis were used: a slime-producing wild type (strain RP62A) and a slime-negative mutant (strain M7). In mixed fungal-bacterial biofilms, both staphylococcal strains showed extensive interactions with C. albicans. The susceptibility of 48-h biofilms to fluconazole, vancomycin and mixtures of the drugs was determined colorimetrically. The results indicated that the extracellular polymer produced by S. epidermidis RP62A could inhibit fluconazole penetration in mixed fungal-bacterial biofilms. Conversely, the presence of C. albicans in a biofilm appeared to protect the slime-negative staphylococcus against vancomycin. Overall, the findings suggest that fungal cells can modulate the action of antibiotics, and that bacteria can affect antifungal activity in mixed fungal-bacterial biofilms.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mixed species biofilms of Candida albicans and Staphylococcus epidermidis

Adam

Baillie

Douglas

2002

Journal of Medical Microbiology

316

223

View full text Add to dashboard Cite

show abstract

“…A biofilm is a structured community of mixed species enclosed in a selfproduced extracellular polymeric matrix formed by exopolysaccharides (EPSs) and adhered on an inert or living surface (Lopez 2010;O´Toole 2000;Watnick 2000). EPSs are composed of a wide variety of species including polysaccharides, proteins, nucleic acid, uronic acid and humic substances.…”

Section: Biofilmmentioning

confidence: 99%

Enzymatic approaches in paper industry for pulp refining and biofilm control

Torres

Negro

Fuente

et al. 2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The use of enzymes has a high potential in the pulp and paper industry to improve the economics of the paper production process and to achieve, at the same time, a reduced environmental impact. Specific enzymes contribute to reduce the amount of chemicals and energy required for the modification of fibers and helps to prevent the formation or development of biofilms. This review is aimed at presenting the latest progresses made in the application of enzymes as refining aids and biofilm control agents.

show abstract

“…One of the earliest observations of such altered behavior was the increased resistance of biofilm cells to antimicrobial agents, biocides, and adverse environmental conditions, including nutrient deprivation (5,7,17). On approaching a surface, bacteria encounter environmental conditions different from those in the bulk liquid phase, a situation known to induce significant alterations in gene expression (8,38). In Pseudomonas aeruginosa, new protein synthesis is required during the initial stage of biofilm formation (6), and the protein expression profile changes 5 min after attachment.…”

mentioning

confidence: 99%

Protein Expression by Streptococcus mutans during Initial Stage of Biofilm Formation

Welin

Wilkins

Beighton

et al. 2004

Appl Environ Microbiol

View full text Add to dashboard Cite

Cells growing on surfaces in biofilms exhibit properties distinct from those of planktonic cells, such as increased resistance to biocides and antimicrobial agents. In spite of increased interest in biofilms, very little is known about alterations in cell physiology that occur upon attachment of cells to a surface. In this study we have investigated the changes induced in the protein synthesis by contact of Streptococcus mutans with a surface. Log-phase planktonic cells of S. mutans were allowed to adhere to a glass slide for 2 h in the presence of a 14 C-amino acid mixture. Nonadhered cells were washed away, and the adhered cells were removed by sonication. The proteins were extracted from the nonadhered planktonic and the adhered biofilm cells and separated by two-dimensional gel electrophoresis followed by autoradiography and image analysis. Image analysis revealed that the relative rate of synthesis of 25 proteins was enhanced and that of 8 proteins was diminished >1.3-fold in the biofilm cells. Proteins of interest were identified by mass spectrometry and computer-assisted protein sequence analysis. Of the 33 proteins associated with the adhesion response, all but 10 were identified by mass spectrometry and peptide mass fingerprinting. The most prominent change in adhered cells was the increase in relative synthesis of enzymes involved in carbohydrate catabolism indicating that a redirection in protein synthesis towards energy generation is an early response to contact with and adhesion to a surface.

show abstract

Biofilm, City of Microbes

Cited by 1,262 publications

References 53 publications

Mixed species biofilms of Candida albicans and Staphylococcus epidermidis

Mixed species biofilms of Candida albicans and Staphylococcus epidermidis

Enzymatic approaches in paper industry for pulp refining and biofilm control

Protein Expression by Streptococcus mutans during Initial Stage of Biofilm Formation

Contact Info

Product

Resources

About