Biofilm Associated Staphylococcal Mastitis in Goats

Unny, N. Madhavan; Pillai, Usha Narayana; Mani, Binu K.

doi:10.5455/ijlr.20200414025108

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…M astitis is a disease caused by a wide range of microorganisms that causes significant economic losses and damages to the dairy industry by reducing milk production, increasing antibiotic treatment, and culling costs (Yanuartono et al, 2019;Unny et al, 2020). It is a complicated condition that develops as a result of interactions between an agent, an animal, and the environment.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Mycotic Mastitis and Evaluation of Some Virulence Potential of Candida albicans Isolated from Mastitic Goats

Mahmoud¹,

Yassein²

2023

AAVS

View full text Add to dashboard Cite

The aims of this study are to identify mycotic agents, assess the prevalence of mycotic mastitis, and evaluate the virulence of C. albicans that was isolated from Mastitic goats. To investigate the prevalence of mycotic mastitis, related to goats in Baghdad, 166 lactating goats were examined and 332 milk samples were collected from ( January -July) 2022. 10 ml of milk were collected in sterile test tubes, an equal volume of milk and CMT reagent were gently shaken in a paddle and the reaction was noticed within (10-15) seconds. The clinical form of mastitis accounted 9.35%, while the subclinical form accounted 52.4%, with positive (+ve) California mastitis test (CMT) results which are high significant. All milk samples were cultured on Sabouraud dextrose agar (SDA) for (3-5) days at 37 ºC. According to the result, 46.34% of the isolates had mycotic mastitis, with 33 isolates (34.74%) belonging to mold and 62 isolates (65.26%) returning to yeasts. Candida albicans had the highest percentage (high significant) of yeast isolation of (67.74%) with 42 isolates which were detected by using conventional methods such as the germ tube test, urease production, Chlamydospore formation, and chromogenic medium. Hemolysis, phospholipase activities, and biofilm formation were among the virulence tested factors. Hemolysis and phospholipase activities were detected in 76.2% and 83.33% of C. albicans isolates, respectively, while biofilm formation was detected in 100% of C. albicans isolates. The diagnosis was confirmed using 25 of the most virulent C. albicans isolates, by polymers chains reaction (PCR) to detect the KER1 gene revealed that all 25 samples were positive with the product size (658bp). Phospholipases B1 (PLB1) and the secretion of aspartyl proteinase 1 (SAP1) gene were used to detect C. albicans virulence with 100% and 80% accuracy, respectively, while Hyphal Wall Protein 1 (HWP1) gene was not detected. It is expected that these C. albicans isolates contained a significant proportion of virulence factors that were linked to pathogenicity and the severity of infection. There findings suggest that poor sanitation and a reduction in goat health and nutrition were to blame for the high morbidity incidence of mycotic mastitis in Iraqi goats.

show abstract

Section: Introductionmentioning

confidence: 99%

Prevalence of Mycotic Mastitis and Evaluation of Some Virulence Potential of Candida albicans Isolated from Mastitic Goats

Mahmoud¹,

Yassein²

2023

AAVS

View full text Add to dashboard Cite

show abstract

“…35, Supplement III, 2021 (73-78) Proceedings of the 13th (2nd International) Scientific Conference, College of Veterinary Medicine, University of Baghdad recognized that continued use of these chemical agents facilitates antibiotic resistant in bacterial communities (4). Multidrug-resistant (MDR) mastitis pathogens, particularly methicillin-resistant staphylococcal strains, are therefore considered a well-documented threat to the production of dairy goats, so treatment that is not successful in the case of mastitis has serious economic effects for the country and for livestock farmers (5). Due to the rise in multidrugresistant infections, researchers revealed that AgNPs had broad range antimicrobial activity, killing over 650 distinct pathogens including viruses, bacteria, parasites, fungus, and molds (6).…”

Section: Introductionmentioning

confidence: 99%

The effectiveness of biogenic silver nanoparticles in the treatment of caprine mastitis induced by Staphylococcus aureus

Dujaily¹,

Mahmood²

2021

IJVS

View full text Add to dashboard Cite

The aim of the present study is to determine the efficiency of silver nanoparticles in mastitis treatment induced by Staphylococcus aureus, 20 lactating goats were infected intramammary inoculated with 1.5×10 8 cfu/ml of virulence Staphylococcus aureus isolated from milk of caprine mastitis and at 48hr post inoculation, the animals were divided randomly into four groups equally, 1 st group left without treatment as control positive, 2 nd group treated intramammary infusion with 5 ml Silver nanoparticle after 2h from infection daily for 3 consecutive days, 3 rd group treated intramammary infusion with 5 ml Silver nanoparticle after 72h from infection daily for 3 consecutive days, 4 th group treated intramammary infusion with 5 ml Silver nanoparticle mixed with gentamycin after 72h from infection daily for 3 consecutive days. The silver nanoparticles used in this study were created using a green method from Bacillus clausii. The Scanning electron microscope was spherical and homogeneous form, with a size range of 25 to 45 nm. Results showed severe clinical signs of the mammary gland with heavy bacterial isolation in 1st control groups at 3, 6-, 9-, 14-and 21-days post infection, while animals that were intramammary treated with Silver nanoparticle alone or combination with gentamycin showed a disappearance in clinical signs of the mammary gland and bacterial isolation, it was concluded that Silver nanoparticle had a strong action against mastitis caused by Staphylococcus aureus.

show abstract

Biofilm Associated Staphylococcal Mastitis in Goats

Cited by 2 publications

References 0 publications

Prevalence of Mycotic Mastitis and Evaluation of Some Virulence Potential of Candida albicans Isolated from Mastitic Goats

Prevalence of Mycotic Mastitis and Evaluation of Some Virulence Potential of Candida albicans Isolated from Mastitic Goats

The effectiveness of biogenic silver nanoparticles in the treatment of caprine mastitis induced by Staphylococcus aureus

Contact Info

Product

Resources

About