Biofilm-associated persistence of food-borne pathogens

Bridier, Arnaud; Sanchez-Vizuete, Pilar; Guilbaud, Morgan; Piard, Jean-Christophe; Naïtali, Murielle; Briandet, Romain

doi:10.1016/j.fm.2014.04.015

Cited by 387 publications

(223 citation statements)

References 180 publications

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“…Most outbreaks whose etiologic agents are transmitted by food seems to be associated with biofilms. In recent years, there have been a significant number of reports correlating the persistence of foodborne pathogens on food contact surfaces and the occurrence of biofilms (Simões and Vieira 2009, Yaron and Römling 2015, Bridier et al 2015. Enterococcus spp.…”

mentioning

confidence: 99%

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Costa

Rossatto

Medeiros

et al. 2018

An. Acad. Bras. Ciênc.

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The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocinlike substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods. The BLS P34 showed inhibitory effect against all planktonic cells of E. faecalis. The inhibition of biofilm formation and the eradication of pre-formed biofilm were evaluated with the crystal violet assay and with the reduction of 3-bromide [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium. The BLS P34 promoted a reduction of percentage of adhered microbial cells on the surface, not being able to perform the complete elimination of biofilm formation. The metabolic activity of S. aureus biofilms decreased considerably between 41-95%. However, E. faecalis cells showed up metabolically stimulated. The BLS P34 has the potential antibiofilm for the species S. aureus. Studies suggest more detailed approaches to a better understanding of the interactions between the antimicrobial and bacterial cells within the biofilm structure.

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mentioning

confidence: 99%

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Costa

Rossatto

Medeiros

et al. 2018

An. Acad. Bras. Ciênc.

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“…stainless steel, polypropylene, rubber and wood) and develop ecosystems called biofilms. This may be a cause of contamination in different industries, as wet surfaces can provide a solid substrate for bacterial growth and persistence (Bridier et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Review on biofilm formation and its control options

2017

Int. J. Adv. Res. Biol. Sci

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The generalized idea that bacteria have a unicellular way of life is not entirely accurate, given that pure planktonic growth is uncommon. Biofilms are defined as an organized group of microorganisms living within a self-produced matrix of polymeric substances which gets attached to several surfaces. This review was done with the objective to give an overview on the mechanism of biofilm formation and highlight its veterinary and public health implications and control options. The formation of a biofilm occurs in five stages, stage of cell attachment, stage securely affixing of cells, stage of micro-colonies formation and beginning to mature, Stage of more maturation, and stage of dispersal of cells from the biofilm. Factors controlling cell attachment include nature of surface, properties of medium and properties of the microbial cell surface. Formation of a biofilm is dependent on the interaction between the environmental stimuli and the reciprocation of the corresponding signalling events by the microorganisms. Biofilms are composed primarily of microbial cells and EPS. The importance of biofilm in disease processes in humans and animals is now widely recognized. In animal species, the risk of infection is probably greater than the risk in humans. In human infections associated with biofilm formation were medical device-related infections including Pacemakers, electrical dialysers, joint prosthetics, intravenous catheters, urinary catheter. As food is identified to be a very efficient vehicle for bringing a large number of people into contact with a potential hazard, food processing equipments can be persistent source of spoilage and pathogenic bacteria if microorganisms form biofilm on them. Ideally, preventing biofilm formation would be a more logical option than treating it. The main strategy to prevent biofilm formation is to clean and disinfect regularly before bacteria attach firmly to surfaces. This process can remove 90% or more of microorganisms associated with the surface. Biofilm detectors, acid shock treatment and recently using bacteriophages have been tried. The prevention and control of biofilm formation is somewhat difficult but prevention is the ideal approach even if there are control methods too. The development of better control and prevention methods with better effect need to be given special emphasis.

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“…Microbial transfer from equipment surfaces to processed foods seems to be responsible of 60% of foodborne infections (Bridier et al, 2015)one of which is the food industry. A solid substrate, water and some nutrients are sufficient to allow the construction of a microbial fortress, a so-called biofilm.…”

Section: Introductionmentioning

confidence: 99%

Bactericidal efficiency of UV-active TiO2 thin films on adhesion and viability of food-borne bacteria

Barthomeuf¹,

Raymond²,

Castel³

et al. 2015

International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

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Biofilms, containing pathogenic bacteria, represent a recurrent economic and safety problem in food industries, due to their high resistance to cleaning and sanitizing procedures. The development of photoactive surfaces with bactericidal property could facilitate the elimination of such microbial biofilms. One solution may be to deposit a photocatalyst top-layer (TiO 2 ) on conventional materials used in food plants. Our aim is to study the photocatalytic activity of such layers on the adhesion and viability of different bacteria present on food plants, especially in pork meat factory: Listeria monocytogenes, Yersinia enterocolitica and Pseudomonas fragi. Glass substrates were coated with TiO 2 thin films by radio-frequency magnetron sputtering under various deposition conditions (deposition temperature T, oxygen partial pressure P O2 ). The characterization of the TiO 2 thin layers was performed using spectrophotometry, scanning electron microscopy and X-ray diffraction analysis. And photocatalytic activity under UVA illumination (365 nm) has been checked for all samples. Bactericidal activity has been demonstrated on the bacteria tested by enumeration of the adherent cells and in situ fluorescent labeling after three hours of contact with the thin film and a subsequent UVA illumination. Adherent bacteria with damaged bacterial cell wall were observed using a scanning electron microscopy; this can be associated with presence of oxidative stress due to the photocatalytic activity of the TiO 2 thin layer. The selected TiO 2 coating presents a photocatalytic activity leading to an oxidative stress. This activity provides bactericidal properties against different strains from the meat industry. This thin layer could be optimized by modifying anionic composition (band-gap reduction) during coating in order to be active under solar light so it could be used to fight against biofilms.

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Biofilm-associated persistence of food-borne pathogens

Cited by 387 publications

References 180 publications

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Review on biofilm formation and its control options

Bactericidal efficiency of UV-active TiO2 thin films on adhesion and viability of food-borne bacteria

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