Biofilm architecture: An emerging synthetic biology target

Kassinger, Stephen J.; Hoek, Monique L. van

doi:10.1016/j.synbio.2020.01.001

Cited by 79 publications

(87 citation statements)

References 212 publications

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“…Bacteria are one of the prime and essential components of nature’s ecosystem, which perform significant macro-level work at the micron-level [ 1 ]. Indigenous to the places from high mountains to deep oceans, cold freezing ice lands to high-temperature volcanic regions, they are arguably the most adaptable living species on Earth [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…Indigenous to the places from high mountains to deep oceans, cold freezing ice lands to high-temperature volcanic regions, they are arguably the most adaptable living species on Earth [ 2 ]. Bacteria exist either in the form of individual cells (planktonic cells) or in the form of biofilms [ 1 , 3 , 4 , 5 , 6 ]. Biofilm is a complex three-dimensional (3D) multilayered structure, formed by multiple planktonic or aggregated bacterial cells by secreting extracellular polymeric substances (EPS) on biological or non-biological surfaces [ 1 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Biofilms are formed by single as well as mixed species of bacteria. Depending on the bacterial strains and environmental factors, biofilms show variable physicochemical properties [ 1 , 3 , 4 , 5 ]. However, irrespective of bacterial species and environment, all biofilms share some common properties.…”

Section: Introductionmentioning

confidence: 99%

“…Depending on growth, availability of nutrients and the surrounding environment, all biofilms acquire a peculiar architecture with varying sizes and shapes (mushroom, flat colonies, pillars, filaments, etc.) [ 1 , 3 ]. Biofilms, irrespective of shapes and sizes, show viscoelastic nature that protect bacteria from mechanical stress [ 1 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…[ 1 , 3 ]. Biofilms, irrespective of shapes and sizes, show viscoelastic nature that protect bacteria from mechanical stress [ 1 , 5 ]. All biofilms show a growth and maturity cycle that starts with adherence of bacterial cells and ends with release of new bacterial cells in the surrounding medium ( Figure 1 A) [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Interactions of Gold and Silver Nanoparticles with Bacterial Biofilms: Molecular Interactions behind Inhibition and Resistance

Joshi

Singh

Mijaković

2020

IJMS

165

113

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Many bacteria have the capability to form a three-dimensional, strongly adherent network called ‘biofilm’. Biofilms provide adherence, resourcing nutrients and offer protection to bacterial cells. They are involved in pathogenesis, disease progression and resistance to almost all classical antibiotics. The need for new antimicrobial therapies has led to exploring applications of gold and silver nanoparticles against bacterial biofilms. These nanoparticles and their respective ions exert antimicrobial action by damaging the biofilm structure, biofilm components and hampering bacterial metabolism via various mechanisms. While exerting the antimicrobial activity, these nanoparticles approach the biofilm, penetrate it, migrate internally and interact with key components of biofilm such as polysaccharides, proteins, nucleic acids and lipids via electrostatic, hydrophobic, hydrogen-bonding, Van der Waals and ionic interactions. Few bacterial biofilms also show resistance to these nanoparticles through similar interactions. The nature of these interactions and overall antimicrobial effect depend on the physicochemical properties of biofilm and nanoparticles. Hence, study of these interactions and participating molecular players is of prime importance, with which one can modulate properties of nanoparticles to get maximal antibacterial effects against a wide spectrum of bacterial pathogens. This article provides a comprehensive review of research specifically directed to understand the molecular interactions of gold and silver nanoparticles with various bacterial biofilms.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Interactions of Gold and Silver Nanoparticles with Bacterial Biofilms: Molecular Interactions behind Inhibition and Resistance

Joshi

Singh

Mijaković

2020

IJMS

165

113

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Metabolic Engineering for Large‐Scale Environmental Bioremediation

Nikel

Lorenzo

2021

Metabolic Engineering

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Synthetic biology in biofilms: Tools, challenges, and opportunities

Tran

Prindle

2021

Biotechnology Progress

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The field of synthetic biology seeks to program living cells to perform novel functions with applications ranging from environmental biosensing to smart cell‐based therapeutics. Bacteria are an especially attractive chassis organism due to their rapid growth, ease of genetic manipulation, and ability to persist across many environmental niches. Despite significant progress in bacterial synthetic biology, programming bacteria to perform novel functions outside the well‐controlled laboratory context remains challenging. In contrast to planktonic laboratory growth, bacteria in nature predominately reside in the context of densely packed communities known as biofilms. While biofilms have historically been considered environmental and biomedical hazards, their physiology and emergent behaviors could be leveraged for synthetic biology to engineer more capable and robust bacteria. Specifically, bacteria within biofilms participate in complex emergent behaviors such as collective organization, cell‐to‐cell signaling, and division of labor. Understanding and utilizing these properties can enable the effective deployment of engineered bacteria into natural target environments. Toward this goal, this review summarizes the current state of synthetic biology in biofilms by highlighting new molecular tools and remaining biological challenges. Looking to future opportunities, advancing synthetic biology in biofilms will enable the next generation of smart cell‐based technologies for use in medicine, biomanufacturing, and environmental remediation.

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Biofilm architecture: An emerging synthetic biology target

Cited by 79 publications

References 212 publications

Interactions of Gold and Silver Nanoparticles with Bacterial Biofilms: Molecular Interactions behind Inhibition and Resistance

Interactions of Gold and Silver Nanoparticles with Bacterial Biofilms: Molecular Interactions behind Inhibition and Resistance

Metabolic Engineering for Large‐Scale Environmental Bioremediation

Synthetic biology in biofilms: Tools, challenges, and opportunities

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