Biofilm activity and sludge characteristics affected by exogenous N-acyl homoserine lactones in biofilm reactors

Hu, Huizhi; He, Junguo; Liu, Jian; Yu, Hui; Zhang, Jie

doi:10.1016/j.biortech.2016.03.068

Cited by 90 publications

(19 citation statements)

References 31 publications

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“…Because the PtraI‐lacZ fusion gene and traR gene are present in strain A136, when exogenous AHLs exist, the PtraI‐lacZ gene is expressed in large quantities by hydrolysing 5‐bromo‐4‐chloro‐3‐indolyl‐β‐ d ‐galacto‐pyranoside (X‐Gal) to produce an obvious blue colour (Hu et al ). Thus, strain A136 and X‐Gal were used to screen for AHLs‐secreting strains.…”

Section: Resultsmentioning

confidence: 99%

Dynamic characteristics of AHLs‐secreting strainAeromonassp. A‐L2 and its bioaugmentation during quinoline biodegradation

Gao

Liu

et al. 2019

J Appl Microbiol

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Aims In order to probe a more environmentally friendly method of pollutant treatment based on microbial bioaugmentation and quorum sensing (QS) effects. Methods and Results The dynamic characteristics and QS effects of the acylated homoserine lactones (AHLs)‐secreting strain Aeromonas sp. A‐L2 (A‐L2), which was isolated from the activated sludge system, was discussed. According to the liquid chromatography‐mass spectrometry results, N‐butyryl‐homoserine lactone (C4‐HSL) and N‐hexanoyl‐homoserine lactone (C6‐HSL) were the major AHLs secreted by strain A‐L2, and the swarming of strain Ochrobactrum sp. LC‐1 (LC‐1) was induced by these compounds. The extracellular polymeric substance secretion of the strain LC‐1 was mainly led by C6‐HSL, and the biofilm formation ability was mainly influenced by C6‐HSL or C4‐HSL (60 μg l−1). The optimal AHLs secretion conditions of strain A‐L2 were also studied. Drawing support from the AHLs‐secreting strain A‐L2 during quinoline degradation by strain LC‐1, the degradation time was greatly shortened. Conclusions Hence, AHLs‐secreting strain A‐L2 can be useful as an AHLs continuous supplier during bioaugmentation and pollutant biodegradation. Significance and Impact of the Study The bioaugmentation process of strain A‐L2 on quinoline biodegradation based on QS effects would lay a certain theoretical and practical significance for large‐scale applications.

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Section: Resultsmentioning

confidence: 99%

Dynamic characteristics of AHLs‐secreting strainAeromonassp. A‐L2 and its bioaugmentation during quinoline biodegradation

Gao

Liu

et al. 2019

J Appl Microbiol

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“…2a), the content of violacein in the extracts from SBBR1 were sharply over 370 units from day 13 to day 16, which indicated that biofilm formed rapidly during this period, whereas the violacein content of SBBR2 stabilized in the range of 147 to 156 units throughout the process, implying a weaker biofilm formation capacity under toxic stress. Drastic changes of violacein content in SBBR1 should be attributed to bioaugmentation with PDB, thereby accelerating biofilm formation, strengthening biosystem resistance and shortening startup time [23]. Combined with higher biofilm biomass (data not shown), SBBR1 maintained a more sufficient degradation capacity, resulting in enhanced pollutant removal.…”

Section: Autoinducer Changes In Sbbrs During Start-upmentioning

confidence: 96%

Bioaugmentation with Phenol-Degrading Bacteria (PDB) as a Strategy for Improving Start-Up and Stability of Sequencing Biofilm Batch Reactor (SBBR) for Coal Gasification Wastewater (CGW) Treatment

Zhuang¹,

Fang²

2020

Pol. J. Environ. Stud.

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Coal gasification wastewater (CGW) is one of the most prevalent industrial effluents in China. It contains hundreds to thousands of milligram phenolic compounds per liter, becoming an important threat to the ecological environment. This study was conducted to evaluate the effectiveness of bioaugmentation with phenol-degrading bacteria (PDB) on improving start-up and stability of sequencing biofilm batch reactor (SBBR) for CGW treatment. The augmented SBBR, with a shortened start-up time of 13 days, showed considerably higher pollutant removal. The superiority of augmented SBBR persisted during the whole operation, with COD, total phenols (TPh) and NH 3 -N efficiencies of 86%, 89% and 35%, respectively, compared to 70%, 75% and 22% for non-augmented SBBR. Under the inhibition of a high concentration of phenolic compounds, the augmented SBBR presented a stronger resistant capability and less recovery time due to inoculating additional PDB. Microbial community analysis revealed that 13.9% of PDB in activated sludge and 10.8% in biofilm as dominant populations, which in turn determined the overall performance of the biosystem, resulting in an efficiently augmented reactor for CGW treatment. The discoveries in this study will facilitate successful development of such bioaugmentation systems and can provide some guidance for practical bioprocesses.

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“…However, the AHLs concentrations in R DF and R UF were much higher than in R C , and the AHLs concentrations in R DF were much higher than in R UF . Hu et al [13] and Li et al [29] reported that QS can severely affect reactor performance, which indicated that higher AHLs concentrations would be beneficial for the removal of nitrogen and COD. This explains why the R DF and R UF nitrogen removal performance was higher than that of R C during this period.…”

Section: Effect Of Fluctuation Hrt On Ahls Produced By Microorganismsmentioning

confidence: 99%

“…Zhang et al [12] reported that HRT could affect microbial community structure, which was closely connected with quorum sensing (QS) systems. In addition, increasing evidence indicated that QS was strongly related to reactor performance, EPS content and sludge granulation [13,14]. A further study showed that QS in activated sludge flocs could regulate both reactor performance and EPS content, and accelerating sludge granulation through a response to changes in the concentration of acyl homoserine lactones (AHLs) [15].…”

Section: Introductionmentioning

confidence: 99%

Effect of fluctuating hydraulic retention time (HRT) on denitrification in the UASB reactors

Niu

Guo

Lian

et al. 2018

Biochemical Engineering Journal

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This study cultivated denitrifying granular sludge in three up-flow anaerobic sludge blanket (UASB) reactors using different fluctuation hydraulic retention time (HRT) strategies (reactor 1 (RC): constant HRT (C-HRT); reactor 2 (RDF): downward fluctuation HRT (DF-HRT); and reactor 3 (RUF): upward fluctuation HRT (UF-HRT)). The results verified that these fluctuation HRT strategies could enhance microbial diversity, while robust against fluctuations in nutrient load of the denitrifying granular sludge. Microbial aggregates appeared in RC, RUF and RDF on days 22, 12 and 7, respectively. The sludge in RUF and RDF achieved complete granulation on days 22 and 14, respectively. Compared to the results of RC and RUF, the acyl homoserine lactones (AHLs) concentration rapidly increased, and changed the components of extracellular polymeric substances (EPS) resulted in the rapid formation of denitrifying granular sludge in RDF. These results demonstrate that microbial community, AHLs, EPS, and the denitrifying sludge granulation process were associated with each other. Informed from quorum sensing system, a mechanism for the granulation of denitrifying sludge using the DF-HRT strategy was proposed. The DF-HRT strategy is an economical and fast method to cultivate denitrifying granular sludge. We hope that the results of our research would provide some theoretical support for wastewater producing unstable plants.

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Biofilm activity and sludge characteristics affected by exogenous N-acyl homoserine lactones in biofilm reactors

Cited by 90 publications

References 31 publications

Dynamic characteristics of AHLs‐secreting strainAeromonassp. A‐L2 and its bioaugmentation during quinoline biodegradation

Dynamic characteristics of AHLs‐secreting strainAeromonassp. A‐L2 and its bioaugmentation during quinoline biodegradation

Bioaugmentation with Phenol-Degrading Bacteria (PDB) as a Strategy for Improving Start-Up and Stability of Sequencing Biofilm Batch Reactor (SBBR) for Coal Gasification Wastewater (CGW) Treatment

Effect of fluctuating hydraulic retention time (HRT) on denitrification in the UASB reactors

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