Biofabrication: Volumetric Bioprinting of Complex Living‐Tissue Constructs within Seconds (Adv. Mater. 42/2019)

Bernal, Paulina Núñez; Delrot, Paul; Loterie, Damien; Li, Yang; Malda, Jos; Moser, Christophe; Levato, Riccardo

doi:10.1002/adma.201970302

Cited by 21 publications

(55 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…38 New SLA systems, also termed volumetric SLA, are capable of cross-linking in a fast way a volume or 3D object by combining the rotation of a resin vat and a DLP projector or laser light source that selectively projects light in a synchronized way. 39,40 This technique has been recently used to demonstrate the capabilities of this new approach to produce bioprinted constructs by including cells within the liquid resin that was cross-linked with a total light exposure time below 1 min. 39 While this technique allows a faster volume manufacturing, no control of the cell spatial distribution can be achieved so far.…”

Section: Light-assisted Bioprintingmentioning

confidence: 99%

“…39,40 This technique has been recently used to demonstrate the capabilities of this new approach to produce bioprinted constructs by including cells within the liquid resin that was cross-linked with a total light exposure time below 1 min. 39 While this technique allows a faster volume manufacturing, no control of the cell spatial distribution can be achieved so far.…”

Section: Light-assisted Bioprintingmentioning

confidence: 99%

See 1 more Smart Citation

Bioprinting: From Tissue and Organ Development to in Vitro Models

et al. 2020

View full text Add to dashboard Cite

Bioprinting techniques have been flourishing in the field of biofabrication with pronounced and exponential developments in the past years. Novel biomaterial inks used for the formation of bioinks have been developed, allowing the manufacturing of in vitro models and implants tested preclinically with a certain degree of success. Furthermore, incredible advances in cell biology, namely, in pluripotent stem cells, have also contributed to the latest milestones where more relevant tissues or organ-like constructs with a certain degree of functionality can already be obtained. These incredible strides have been possible with a multitude of multidisciplinary teams around the world, working to make bioprinted tissues and organs more relevant and functional. Yet, there is still a long way to go until these biofabricated constructs will be able to reach the clinics. In this review, we summarize the main bioprinting activities linking them to tissue and organ development and physiology. Most bioprinting approaches focus on mimicking fully matured tissues. Future bioprinting strategies might pursue earlier developmental stages of tissues and organs. The continuous convergence of the experts in the fields of material sciences, cell biology, engineering, and many other disciplines will gradually allow us to overcome the barriers identified on the demanding path toward manufacturing and adoption of tissue and organ replacements.

show abstract

Section: Light-assisted Bioprintingmentioning

confidence: 99%

Section: Light-assisted Bioprintingmentioning

confidence: 99%

Bioprinting: From Tissue and Organ Development to in Vitro Models

et al. 2020

View full text Add to dashboard Cite

show abstract

“…11 Most recently, bioresins have also been applied to computed axial lithography (CAL) bioprinting, a technique that permits the rapid and selective photo-cross-linking of a material within a contained volume so that arbitrary geometries are formed through controlled light exposure. 14 Despite significant research advances in photo-cross-linking approaches and techniques in biofabrication for regenerative medicine and in vitro 3D tissue models, in combination with a number of existing reviews that focus primarily on the polymerization of hydrogels, 15,16 there is limited information available that reviews the key fundamentals of photo-crosslinking and necessary compositional and processing criteria for the successful design and implementation of photo-cross-linked bioinks and bioresins.…”

Section: Introductionmentioning

confidence: 99%

Fundamentals and Applications of Photo-Cross-Linking in Bioprinting

et al. 2020

View full text Add to dashboard Cite

This review provides a detailed overview of the rapidly advancing field of biofabrication, particularly with regards to the use of photo-cross-linking (i.e., light-based) techniques. The major emphasis of this review is on the fundamentals of photo-cross-linking and key criteria identified for the successful design and implementation of photo-cross-linked bioinks and bioresins in extrusion-based and lithography-based bioprinting. The general mechanisms associated with photo-cross-linking (e.g., free-radical chain polymerization, thiol–ene, photomediated redox) of natural and synthetic materials are described to inform bioink and bioresin design, which includes the selection of polymers, functional group modifications, photoinitiators, and light sources that enable facile and cytocompatible photo-cross-linking. Depending on material selection and the bioprinting technique of interest, we describe the specific bioink or bioresin properties and criteria that must be achieved to ensure optimal printability and utility. Finally, examples of current state-of-the-art applications of light-based bioprinting for in vitro tissue models, tissue engineering, and regenerative medicine are provided to further motivate future opportunities within the bioprinting landscape that are facilitated with light.

show abstract

“…I) A schematic of how spheroids (blue) positioned with the Kenzan method could be further entrapped with a bioresin. (A-I) reproduced with permission [109]. Copyright 2019, The Authors, Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.…”

mentioning

confidence: 99%

Advances in Hybrid Fabrication toward Hierarchical Tissue Constructs

et al. 2020

View full text Add to dashboard Cite

The diversity of manufacturing processes used to fabricate 3D implants, scaffolds, and tissue constructs is continuously increasing. This growing number of different applicable fabrication technologies include electrospinning, melt electrowriting, volumetric‐, extrusion‐, and laser‐based bioprinting, the Kenzan method, and magnetic and acoustic levitational bioassembly, to name a few. Each of these fabrication technologies feature specific advantages and limitations, so that a combination of different approaches opens new and otherwise unreachable opportunities for the fabrication of hierarchical cell–material constructs. Ongoing challenges such as vascularization, limited volume, and repeatability of tissue constructs at the resolution required to mimic natural tissue is most likely greater than what one manufacturing technology can overcome. Therefore, the combination of at least two different manufacturing technologies is seen as a clear and necessary emerging trend, especially within biofabrication. This hybrid approach allows more complex mechanics and discrete biomimetic structures to address mechanotransduction and chemotactic/haptotactic cues. Pioneering milestone papers in hybrid fabrication for biomedical purposes are presented and recent trends toward future manufacturing platforms are analyzed.

show abstract

Biofabrication: Volumetric Bioprinting of Complex Living‐Tissue Constructs within Seconds (Adv. Mater. 42/2019)

Cited by 21 publications

References 0 publications

Bioprinting: From Tissue and Organ Development to in Vitro Models

Bioprinting: From Tissue and Organ Development to in Vitro Models

Fundamentals and Applications of Photo-Cross-Linking in Bioprinting

Advances in Hybrid Fabrication toward Hierarchical Tissue Constructs

Contact Info

Product

Resources

About