Biofabrication of Zinc Oxide Nanoparticles from Two Different Zinc Sources and Their Antimicrobial Activity

Khajuria, Arun Kumar; Kumari, Monika; Kandwal, Anuj; Singh, Abhay Kumar; Bisht, N. S.

doi:10.1007/s12668-020-00802-5

Cited by 9 publications

(5 citation statements)

References 49 publications

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“…Gram-positive bacteria were more sensitive than Gram-negative bacteria to ZnO NPs. This is in agreement with many studies reporting that the Gram-positive bacteria are more in danger of being attacked by ZnO NPs than Gram-negative bacteria which is in agreement with the proposed antibacterial mechanism of ZnO operation [11,57,58]. ZnO NPs create enough ROS to damage the bacterial cell structures.…”

Section: Antimicrobial Activity Of Zno Npssupporting

confidence: 92%

Synthesis of ZnO Nanoparticles with Antibacterial Properties Using Terminalia catappa Leaf Extract

2022

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In order to develop alternative drugs against resistant pathogens, capped ZnO nanoparticles (NPs) were prepared using Terminalia catappa leaf extract. By changes in pH and aging time, cauliflower-like, sheet-like, and hierarchical morphologies for ZnO NPs were obtained. Their antibacterial activities against Staphylococcus aureus and Escherichia coli were investigated. The results demonstrated that the green synthesized ZnO NPs exhibit higher antibacterial activity than chemically prepared ZnO NPs. The antibacterial activity of hierarchical ZnO NPs was greater than that of the other morphologies, which was attributed to the higher oxygen vacancy in ZnO NPs leading to enhanced reactive oxygen species (ROS) generation. The mechanism of the formation of ZnO nanocrystals was also discussed.

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Section: Antimicrobial Activity Of Zno Npssupporting

confidence: 92%

Synthesis of ZnO Nanoparticles with Antibacterial Properties Using Terminalia catappa Leaf Extract

2022

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“…This confirmed the presence of the plant extract secondary metabolites on the surface of the nanoparticles playing the roles of capping and stabilizing agents. Further, the absorption peaks detected at (3435.15 and 3435.55 cm −1 ), (2078.88 and 2075.34 cm −1 ), (1633.85 and 1634.21 cm −1 ), and (709.90 and 666.84 cm −1 ) are, respectively, ascribed to alcohol OH, alkynes, carboxylic acids, and aromatic compounds as functional groups [ 74 , 75 , 76 ]. According to [ 77 ], the slight shifting peaks between the plant extract and the synthesized nanoparticles are owing to the decrease in the value of the force constant and the increase in the reduced mass, which can simultaneously act to provoke a shift in the FTIR peaks.…”

Section: Discussionmentioning

confidence: 99%

“…Besides having an organogenesis fate, callus also has the potential for the same chemical as the plant and can, therefore, be used for secondary metabolites production [ 76 ]. The in vitro regeneration of medicinal plants is used by industrial companies to produce bioactive compounds in a relatively short period of time for ensuring continuous supply [ 87 ].…”

Section: Discussionmentioning

confidence: 99%

Improving the Production of Secondary Metabolites via the Application of Biogenic Zinc Oxide Nanoparticles in the Calli of Delonix elata: A Potential Medicinal Plant

et al. 2023

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The implementation of nanotechnology in the field of plant tissue culture has demonstrated an interesting impact on in vitro plant growth and development. Furthermore, the plant tissue culture accompanying nanoparticles has been showed to be a reliable alternative for the biosynthesis of secondary metabolites. Herein, the effectiveness of zinc oxide nanoparticles (ZnONPs) on the growth of Delonix elata calli, as well as their phytochemical profiles, were investigated. Delonix elata seeds were collected and germinated, and then the plant species was determined based on the PCR product sequence of ITS1 and ITS4 primers. Afterward, the calli derived from Delonix elata seedlings were subjected to 0, 10, 20, 30, 40, and 50 mg/L of ZnONPs. The ZnONPs were biologically synthesized using the Ricinus communis aqueous leaf extract, which acts as a capping and reducing agent, and zinc nitrate solution. The nanostructures of the biogenic ZnONPs were confirmed using different techniques like UV–visible spectroscopy (UV), zeta potential measurement, Fourier transform infrared spectra (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). Adding 30 mg/L of ZnONPs to the MS media (containing 2.5 µM 2,4-D and 1 µM BAP) resulted in the highest callus fresh weight (5.65 g) compared to the control and other ZnONP treatments. Similarly, more phenolic accumulation (358.85 µg/g DW) and flavonoid (112.88 µg/g DW) contents were achieved at 30 mg/L. Furthermore, the high-performance liquid chromatography (HPLC) analysis showed significant increments in gallic acid, quercetin, hesperidin, and rutin in all treated ZnONP calli compared to the control. On the other hand, the gas chromatography and mass spectroscopy (GC-MS) analysis of the calli extracts revealed that nine phytochemical compounds were common among all extracts. Moreover, the most predominant compound found in calli treated with 20, 30, 40, and 50 mg/L of ZnONPs was bis(2-ethylhexyl) phthalate, with percentage areas of 27.33, 38.68, 22.66, and 17.98%, respectively. The predominant compounds in the control and in calli treated with 10 mg/L of ZnONPs were octadecanoic acid, 2-propenyl ester and heptanoic acid. In conclusion, in this study, green ZnONPs exerted beneficial effects on Delonix elata calli and improved their production of bioactive compounds, especially at a dose of 30 mg/L.

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“…Peaks at 1240 cm −1 and 1100 cm −1 may be attributed to stretching vibrations of C-O bonds in the ether and ester groups present in the AI leaves. All these bands indicate the presence of different phytochemicals, such as polyols (phenolic acid and flavonoids), terpenoids and protein compounds in AI leaf extract [37,[39][40][41]. Finally, in the case of ZnO-AI nanocore-shell particles (figure 5(b)), it is observed that all the peaks corresponding to diverse functional groups of phytochemicals (indicated by green colour) coexist alongside the distinctive peaks characterising ZnO NPs (indicated by red colour).…”

Section: Ftir Spectroscopymentioning

confidence: 91%

“…Bands at 870 cm −1 and 690 cm −1 are typically associated with stretching and bending vibrations of Zn-O bond, confirming the formation of ZnO crystal. Moreover, the broad bands near 3500 cm −1 and 1390 cm −1 correspond to stretching vibrations of the hydroxyl group (O-H bond) [36,37].…”

Section: Ftir Spectroscopymentioning

confidence: 99%

Azadirachta indica (AI) leaf extract coated ZnO-AI nanocore–shell particles for enhanced antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA)

Sharma,

Upadhyaya,

Deshmukh

et al. 2024

Biomed. Mater.

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With the rise in microbial resistance to traditional antibiotics and disinfectants, there is a pressing need for the development of novel and effective antibacterial agents. Two major approaches being adopted worldwide to overcome antimicrobial resistance are use of plant leaf extracts and metallic nanoparticles, but there is no report on the antibacterial potential of plant extract coated nano particles, which may lead to novel ways of treating infections. This study presents an innovative approach to engineer antibacterial nanoparticles by leveraging the inherent antibacterial properties of Zinc Oxide nanoparticles (ZnO NPs) in combination with Azadirachta indica (AI) leaf extract, resulting in enhanced antibacterial efficacy. ZnO NPs were synthesized by precipitation method and subsequently coated with AI leaf extract to produce ZnO-AI nanocore-shell structures. The structural and morphological characteristics of the bare and leaf extract coated ZnO NPs were analysed by x-ray diffraction (XRD) and field emission scanning electron microscopy (FESEM), respectively. Confirmation of coating of AI leaf extract onto ZnO NPs and subsequent formation of ZnO-AI nanocore-shell structures was accomplished through Fourier transform infrared (FTIR) spectroscopy and photoluminescence (PL) techniques. The antibacterial efficacy of both ZnO NPs and ZnO-AI nanocore-shell particles were evaluated against Methicillin-Resistant Staphylococcus aureus (MRSA) using zone of inhibition assay. The results showed a nanoparticle concentration-dependent increase in the diameter of the inhibition zone, with ZnO-AI nanocore-shell particles exhibiting superior antibacterial properties, owing to the combined effect of ZnO NPs and the poly phenols present in AI leaf extract. These findings suggest ZnO-AI nanocore-shell structures hold promise for the development of novel antibacterial creams and hydrogels for various biomedical applications.

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Biofabrication of Zinc Oxide Nanoparticles from Two Different Zinc Sources and Their Antimicrobial Activity

Cited by 9 publications

References 49 publications

Synthesis of ZnO Nanoparticles with Antibacterial Properties Using Terminalia catappa Leaf Extract

Synthesis of ZnO Nanoparticles with Antibacterial Properties Using Terminalia catappa Leaf Extract

Improving the Production of Secondary Metabolites via the Application of Biogenic Zinc Oxide Nanoparticles in the Calli of Delonix elata: A Potential Medicinal Plant

Azadirachta indica (AI) leaf extract coated ZnO-AI nanocore–shell particles for enhanced antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA)

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