Biofabrication of Silver Nanoparticles Using Leaves of Gloriosa Superba and Its Anticancer Properties

Abstract

Objective: We aimed to synthesize the cost effective, one pot and an eco-friendly technique for the green synthesis of silver nanoparticles (AgNPs) using 1 mM of silver nitrate (AgNO 3 ) solution through the aqueous leaf extracts of Gloriosa superba (GS) reducing and capping agent and its anticancer activity.Methods: Synthesis briefly 95 mL of 1 mM AgNO 3 was taken into amber colored conical flask and added 5 mL of aqueous leaf extract of GS (pale brown) and incubated at room temperature in dark condition for about 24 hrs. Characterization of AgNPs derived from GS (GS-AgNPs) was performed with physiochemical techniques (ultraviolet, transmission electron microscope [TEM], X-ray diffraction [XRD], and thermal gravimetric analysis) and cytotoxicity by 3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyltetrazolium bromide assay. Results:We synthesized cost effective, eco-friendly AgNPs were characterized by physiochemical techniques. The crystal nature of AgNP was studied by XRD. TEM studies reveal the morphology of GS-AgNPs, the size of the nanoparticle is 10-50 nm. The cytotoxicity of GS-AgNPs studied against the four human cancerous cell line DU145, SKOV3, PC3, and A549 but the GS-AgNPs are most sensitive toward the SKOV3 cell line. The minimum inhibitory concentration (IC) is 79. 45±5.26, 61.80±4.27, 94.74±9.26, and 90.10±8.24 µg/mL, respectively. Morphological assessment of the SKOV3 cells was studied using AO/EB and Hoechst staining at IC 50 concentration. Conclusion:The bio fabrication of the GS-AgNPs were simple, eco-friendly and one pot synthesis, it is used as an anticancer agent in future, pending further investigation.

Antiproliferiative Activity of Biogenic Silver Nanoparticles Synthesized from Leonotis nepetifolia (L) on Human Cancer Cell lines

“…LnAgNPs had exhibited high antiproliferiative activity over that of the LNELE and commercially available AgNPs. It was found that in a literature, the aqueous leaf extracts of Rhynchosia suaveolens and Gloriosa superba mediated AgNPs showed potent cytotoxic activity at a concentration (IC 50 ) of 4.2 and 61.80±4.27 µg/ml on SK-OV-3 cell lines [6,50]. Further, it was reported that the ethanolic leaf extract of Ocimum basilicum showed cytotoxicity at a concentration of 5 mg/ml within 72 h with an IC 50 value of 0.91±0.11 mg/ml on SK-OV-3 cell lines [51].…”