Biofabrication of Cellular Structures Using Weightlessness as a Biotechnological Tool

Anıl-İnevi, Müge; Yalcin-Ozuysal, Özden; Sarigil, Oyku; Meşe, Gülistan; Özçivici, Engin; Yaman, Sena; Tekin, H. Cumhur

doi:10.1109/rast.2019.8767431

Cited by 8 publications

(10 citation statements)

References 15 publications

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“…Also, it was shown that sickle cell disease which results in increased density of erythrocytes (Knowlton et al, 2015) and drug treatments that cause density changes in bacteria and yeast (Durmus et al, 2015) can be tested with magnetic levitation systems and portable platforms of the system enable applications for point of care testing (Stephanie Knowlton et al, 2017; Yenilmez, Knowlton, & Tasoglu, 2016; Yenilmez, Knowlton, Yu, et al, 2016). After the successful application of the magnetic levitation system to single cell‐based studies, it became a focus of interest as a biofabrication tool for tissue engineering (Anil‐Inevi, Yalcin‐Ozuysal, et al, 2019; Mishriki et al, 2019). Previously, the suitability of a long‐term cell culture in magnetic levitation platform was demonstrated by single and coculture culture of cancer and stem cells (Anil‐Inevi et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Scaffold‐free biofabrication of adipocyte structures with magnetic levitation

Sarigil

Anıl-İnevi

Firatligil-Yildirir

et al. 2020

Biotech & Bioengineering

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Tissue engineering research aims to repair the form and/or function of impaired tissues. Tissue engineering studies mostly rely on scaffold‐based techniques. However, these techniques have certain challenges, such as the selection of proper scaffold material, including mechanical properties, sterilization, and fabrication processes. As an alternative, we propose a novel scaffold‐free adipose tissue biofabrication technique based on magnetic levitation. In this study, a label‐free magnetic levitation technique was used to form three‐dimensional (3D) scaffold‐free adipocyte structures with various fabrication strategies in a microcapillary‐based setup. Adipogenic‐differentiated 7F2 cells and growth D1 ORL UVA stem cells were used as model cells. The morphological properties of the 3D structures of single and cocultured cells were analyzed. The developed procedure leads to the formation of different patterns of single and cocultured adipocytes without a scaffold. Our results indicated that adipocytes formed loose structures while growth cells were tightly packed during 3D culture in the magnetic levitation platform. This system has potential for ex vivo modeling of adipose tissue for drug testing and transplantation applications for cell therapy in soft tissue damage. Also, it will be possible to extend this technique to other cell and tissue types.

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Section: Discussionmentioning

confidence: 99%

Scaffold‐free biofabrication of adipocyte structures with magnetic levitation

Sarigil

Anıl-İnevi

Firatligil-Yildirir

et al. 2020

Biotech & Bioengineering

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“…Diamagnetic levitation is another laboratory-based method to simulate microgravity on earth. While clinostats, RWV, and RPM cancel the gravity vector through rotation over the period, diamagnetic levitation counteracts the gravitational force (𝐹 𝑔 ) by levitating the object with magnetic force (𝐹 𝑚 ) [85]. The force Fm acting on an object is given as [85],…”

Section: Random Positioning Machinementioning

confidence: 99%

Lab-On-a-Chip Technologies for Microgravity Simulation and Space Applications

Vashi¹,

Sreejith²,

Nguyen³

2022

Preprint

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Gravity played an important role in the development of life on earth. The effect of gravity on a living organisms can be investigated by controlling the magnitude of gravity. Most reduced gravity experiments are conducted on the lower earth orbit (LEO) in the International Space Station (ISS). However, running experiments in ISS face challenges such as high cost, extreme condition, lack of direct accessibility, and long waiting period. Therefore, researchers have developed various ground-based devices and methods to perform reduced gravity experiments. However, the search for life outside the earth requires more intensive research. Advancements in conventional methods and the development of new tools are required for this purpose. The advantages of Lab-on-a-Chip (LOC) devices make them an attractive option for simulating microgravity. This paper briefly reviews the advancement of LOC technologies for simulating microgravity in a earth-based laboratory.

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“…This system levitates materials in paramagnetic solutions under a low magnetic field (<0.5 T) that is generated by two rectangular permanent magnets with the same poles facing each other (Anil‐Inevi et al,2019b; Durmus et al,2015; Mirica et al,2009). However, this setup only allows biofabrication applications in microcapillaries, limiting working volumes for cells (Anil‐Inevi et al,2018,2019a; Sarigil et al,2020; Türker et al,2018). Increasing the size of living structures is of prime importance for straightforward implementation of testing protocols by providing an adequate number of cells (Menasche et al,2019; Shen et al,2017; Van Peer et al,2012), and for the production of sizable tissue engineering constructs (Morrison et al,2016; Park et al,2019).…”

Section: Introductionmentioning

confidence: 99%

Magnetic levitation assisted biofabrication, culture, and manipulation of 3D cellular structures using a ring magnet based setup

Anıl-İnevi

Delikoyun

Meşe

et al. 2021

Biotech & Bioengineering

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Diamagnetic levitation is an emerging technology for remote manipulation of cells in cell and tissue level applications. Low-cost magnetic levitation configurations using permanent magnets are commonly composed of a culture chamber physically sandwiched between two block magnets that limit working volume and applicability. This work describes a single ring magnet-based magnetic levitation system to eliminate physical limitations for biofabrication. Developed configuration utilizes sample culture volume for construct size manipulation and long-term maintenance. Furthermore, our configuration enables convenient transfer of liquid or solid phases during the levitation. Before biofabrication, we first calibrated/ the platform for levitation with polymeric beads, considering the single cell density range of viable cells. By taking advantage of magnetic focusing and cellular self-assembly, millimeter-sized 3D structures were formed and maintained in the system allowing easy and on-site intervention in cell culture with an open operational space. We demonstrated that the levitation protocol could be adapted for levitation of various cell types (i.e., stem cell, adipocyte and cancer cell) representing cells of different densities by modifying the paramagnetic ion concentration that could be also reduced by manipulating the density of the medium. This technique allowed the manipulation and merging of separately formed 3D biological units, as well as the hybrid biofabrication with biopolymers. In conclusion, we believe that this platform will serve as an important tool in broad fields such as bottom-up tissue engineering, drug discovery and developmental biology.

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Biofabrication of Cellular Structures Using Weightlessness as a Biotechnological Tool

Cited by 8 publications

References 15 publications

Scaffold‐free biofabrication of adipocyte structures with magnetic levitation

Scaffold‐free biofabrication of adipocyte structures with magnetic levitation

Lab-On-a-Chip Technologies for Microgravity Simulation and Space Applications

Magnetic levitation assisted biofabrication, culture, and manipulation of 3D cellular structures using a ring magnet based setup

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