Bioethanol production from woody stem Prosopis juliflora using thermo tolerant yeast Kluyveromyces marxianus and its kinetics studies

Sivarathnakumar, S.; Jayamuthunagai, J.; Baskar, G.; Praveenkumar, R.; Selvakumari, I. Aberna Ebenezer; Bharathiraja, B.

doi:10.1016/j.biortech.2019.122060

Cited by 47 publications

(9 citation statements)

References 27 publications

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“…The maximum ethanol concentration in the K. marxianus SSF cultures on raw starch reached 12.11 ± 0.26 g/L (Figure 4A). When compared with the other literature reports using comparable substrate load (< 10%), here obtained titers are comparable to 14 g/L achieved under oxygen-limited conditions in [43], but is also 2-fold lower than the ethanol concentrations reported in [58,59]. In the other studies, when a higher load of the substrate was implemented, the ethanol concentration reached 49 g/L [44], 80 g/L [57] or even 110 g/L, obtained in our previous studies [8].…”

Section: Discussionsupporting

confidence: 73%

Production of Raw Starch-Digesting Amylolytic Preparation in Yarrowia lipolytica and Its Application in Biotechnological Synthesis of Lactic Acid and Ethanol

et al. 2020

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Sustainable economy drives increasing demand for raw biomass-decomposing enzymes. Microbial expression platforms exploited as cellular factories of such biocatalysts meet requirements of large-volume production. Previously, we developed Yarrowia lipolytica recombinant strains able to grow on raw starch of different plant origin. In the present study, we used the most efficient amylolytic strain as a microbial cell factory of raw-starch-digesting (RSD) amylolytic preparation composed of two enzymes. The RSD-preparation was produced in fed-batch bioreactor cultures. Concentrated and partly purified preparation was then tested in simultaneous saccharification and fermentation (SSF) processes with thermotolerant Kluyveromyces marxianus for ethanol production and Lactobacillus plantarum for production of lactic acid. These processes were conducted as a proof-of-concept that application of the novel RSD-preparation supports sufficient starch hydrolysis enabling microbial growth and production of targeted molecules, as the selected strains were confirmed to lack amylolytic activity. Doses of the preparation and thermal conditions were individually adjusted for the two processes. Additionally, ethanol production was tested under different aeration strategies; and lactic acid production process was tested in thermally pre-treated substrate, as well. Conducted studies demonstrated that the novel RSD-preparation provides satisfactory starch hydrolyzing activity for ethanol and lactic acid production from starch by non-amylolytic microorganisms.

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Section: Discussionsupporting

confidence: 73%

Production of Raw Starch-Digesting Amylolytic Preparation in Yarrowia lipolytica and Its Application in Biotechnological Synthesis of Lactic Acid and Ethanol

et al. 2020

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“…In contrast, fermentation of K. marxianus in some poor media was regarded as promising industrial applications. For example, K. marxianus was utilized in bioethanol production from cheese whey permeate (Silveira et al, 2005;Zafar and Owais, 2006), and lignocellulosic biomass (Sivarathnakumar et al, 2019). K. marxianus was developed to remove copper(II) ions from wastewater in the presence of molasses (Skountzou et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomic and Transcriptomic Analysis Reveals Specific Features of Gene Regulation in Kluyveromyces marxianus

Yang

et al. 2021

Front. Microbiol.

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Kluyveromyces marxianus is a promising host for producing bioethanol and heterologous proteins. It displays many superior traits to a conventional industrial yeast species, Saccharomyces cerevisiae, including fast growth, thermotolerance and the capacity to assimilate a wider variety of sugars. However, little is known about the mechanisms underlying the fast-growing feature of K. marxianus. In this study, we performed a comparative genomic analysis between K. marxianus and other Saccharomycetaceae species. Genes involved in flocculation, iron transport, and biotin biosynthesis have particularly high copies in K. marxianus. In addition, 60 K. marxianus specific genes were identified, 45% of which were upregulated during cultivation in rich medium and these genes may participate in glucose transport and mitochondrion related functions. Furthermore, the transcriptomic analysis revealed that under aerobic condition, normalized levels of genes participating in TCA cycles, respiration chain and ATP biosynthesis in the lag phase were higher in K. marxianus than those in S. cerevisiae. Levels of highly copied genes, genes involved in the respiratory chain and mitochondrion assembly, were upregulated in K. marxianus, but not in S. cerevisiae, in later time points during cultivation compared with those in the lag phase. Notably, during the fast-growing phase, genes involved in the respiratory chain, ATP synthesis and glucose transport were co-upregulated in K. marxianus. A few shared motifs in upstream sequences of relevant genes might result in the co-upregulation. Specific features in the co-regulations of gene expressions might contribute to the fast-growing phenotype of K. marxianus. Our study underscores the importance of genome-wide rewiring of the transcriptional network during evolution.

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“…Prosopis juliflora (Sw.) DC is rich in holocellulose (60 -65 %), therefore, it is considered for bioethanol production [80,[97][98][99]. In such a study, Sivarathnakumar et al [99] used Kluyveromyces marxianus leading to the increased sugar content by applying auto-hydrolysis with acid hydrolysis as a pre-treatment.…”

Section: Biofuel Productionmentioning

confidence: 99%

Energy plants as biofuel source and as accumulators of heavy metals

Nikolić

Tomašević

Ugrinov

2022

Hem Ind

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Fossil fuel depletion and soil and water pollution gave impetus to the development of a novel perspective of sustainable development. In addition to the use of plant biomass for ethanol production, plants can be used to reduce the concentration of heavy metals in soil and water. Due to tolerance to high levels of metals, many plant species, crops, non-crops, medicinal, and pharmaceutical energy plants are well-known metal hyperaccumulators. This paper focuses on studies investigating the potential of Miscanthus sp., Beta vulgaris L., Saccharum sp., Ricinus communis L. Prosopis sp. and Arundo donax L. in heavy metal removal and biofuel production. Phytoremediation employing these plants showed great potential for bioaccumulation of Co, Cr, Cu, Al, Pb, Ni, Fe, Cd, Zn, Hg, Se, etc. This review presents the potential of lignocellulose plants to remove pollutants being a valuable substrate for biofuel production. Also, pretreat-ments, dealing with toxic biomass, and biofuel production are discussed.

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Bioethanol production from woody stem Prosopis juliflora using thermo tolerant yeast Kluyveromyces marxianus and its kinetics studies

Cited by 47 publications

References 27 publications

Production of Raw Starch-Digesting Amylolytic Preparation in Yarrowia lipolytica and Its Application in Biotechnological Synthesis of Lactic Acid and Ethanol

Production of Raw Starch-Digesting Amylolytic Preparation in Yarrowia lipolytica and Its Application in Biotechnological Synthesis of Lactic Acid and Ethanol

Comparative Genomic and Transcriptomic Analysis Reveals Specific Features of Gene Regulation in Kluyveromyces marxianus

Energy plants as biofuel source and as accumulators of heavy metals

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