Bioethanol production from wheat straw via enzymatic route employing Penicillium janthinellum cellulases

Singhania, Reeta Rani; Saini, Jitendra Kumar; Saini, Reetu; Adsul, Mukund; Mathur, Anshu S.; Gupta, Ravi P.; Tuli, Deepak K.

doi:10.1016/j.biortech.2014.07.011

Cited by 93 publications

(38 citation statements)

References 32 publications

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“…Additionally, continuous removal of sugars from the medium minimizes the inhibition of enzyme activity by the feedstock. This resulted in enhanced saccharification and lactic acid yield in SSF relative to those in SHF (Singhania et al 2014). L-lactic acid production in SSF was 17% higher than that in SHF.…”

Section: Comparison Of Ssf and Shf In Open L-lactic Acid Fermentationmentioning

confidence: 98%

Open Fermentative Production of L-Lactic Acid from Distillers’ Grains by Lactobacillus casei CICC 6056

et al. 2016

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Distillers' grains (DG) are potential fermentable sugar substrates for the production of value-added products. This study focused on the development of an open fermentation process for the production of Llactic acid from DG using Lactobacillus casei CICC 6056. The open fermentation process was feasible, resulting in an L-lactic acid yield similar to that obtained with sterilized fermentation. However, a decrease in pH below 5.0 after 24 h resulted in a poor L-lactic acid concentration of 9.18 g/L and an inferior reducing sugar conversion rate of 53.6%. Therefore, an optimal pH adjustment method was sought. A pH adjustment to 6.5 every 24 h effectively improved the L-lactic acid concentration to 21.3 g/L with a 97.8% reducing sugar conversion rate. Furthermore, the application of a simultaneous saccharification and fermentation process at 50 °C in open condition increased the L-lactic acid concentration to 25.0 g/L. This yield was superior to L-lactic acid fermentation from DG using a separate hydrolysis and fermentation method (21.3 g/L) and from a commercial sugar in batch culture (23.6 g/L). These results demonstrated that L. casei CICC 6056 has great industrial potential as a superior candidate strain for the production of Llactic acid from DG due to its broad applicability in a wide temperature range (from 37 to 50 °C).

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Section: Comparison Of Ssf and Shf In Open L-lactic Acid Fermentationmentioning

confidence: 98%

Open Fermentative Production of L-Lactic Acid from Distillers’ Grains by Lactobacillus casei CICC 6056

et al. 2016

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“…Results showed that maximum hydrolysis yield (>95%) was achieved with OptEMix and CL enzyme. Singhania, et al 38 found that about 51% hydrolysis of dilute acid pretreated wheat straw was achieved with 7.34 mg protein per g of biomass using Alvira, et al 36 have found that approx.…”

Section: Enzymatic Hydrolysis Of Pws With Different Commercial Enzymesmentioning

confidence: 99%

Improved saccharification of pilot-scale acid pretreated wheat straw by exploiting the synergistic behavior of lignocellulose degrading enzymes

et al. 2015

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Requirement of high enzyme dosage for lignocellulosic biomass hydrolysis is one of the challenges for the viability of the second generation bioethanol technology. Here, an optimal enzyme mixture was developed by partially replacing the cellulase proportion with accessory enzymes (b-glucosidase, xylanase, pectinase, laccase) and its hydrolytic performance was compared with different commercial counterparts for the saccharification of pretreated wheat straw (PWS) using a 250 kg per day continuous pilot plant. Maximum degree of synergism was observed with xylanase followed by pectinase, laccase, and bglucosidase. The statistically optimized enzyme mixture enhanced hydrolysis by 51.23% and 40.66% in 6 h and 24 h, respectively. This study elucidates that presence of even small amount of oligomers and cellobiose pose a strong inhibition for the enzymes. Therefore, development of an optimal enzyme formulation is a sustainable approach to reduce overall enzyme loading for biomass saccharification.

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“…Adsul et al (2014) found that a mutant of P. janthinellum showed high production of cellulases in solid state fermentation. Singhania et al (2014) demonstrated that P. janthinellum EMS-UV-8 is a fungus with potential for future largescale production of cellulase. Liao (2014) reported induced high-efficiency lignocellulolytic enzyme production by Penicillium oxalicum on complex substrates.…”

Section: Lignocellulolytic Activity Of Selected Isolates In Solid Stamentioning

confidence: 99%

Crop Residue Degradation by Autochthonous Fungi Isolated from Cropping System Management Scenarios

2015

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In the rice-wheat system dominated belt of India (Indo-Gangetic plains; IGP), paddy leaves, about 8 to 9 t/ha of loose and anchored residue in the field, are mechanically harvested. Farmers prefer to burn this residue to clear the field for the timely preparation of conventional wheat sowing. Insitu degradation by autochthonous fungi can be a better option for the disposal of unwanted crop residues. Autochthonous fungi isolated from conservation agriculture-based crop management fields were screened and assessed for their residue degradation potential. Nineteen isolates were selected for detailed enzymatic analysis in submerged fermentation, responsible for lignocellulosic residue degradation. Out of these five fungal isolates RPW 1/3 (Aspergillus flavus), RPW 1/6 (Aspergillus terreus), RPW 1/9 (Aspergillus terreus), RPWM 2/2 (Penicillium janthinellum) and RZWM 3/1 (Aspergillus niger) showed higher activities of cellobiase, CMCase, FPase, xylanase, and laccase enzymes in solid state fermentation. Further two isolates RPW 1/3 and 1/6 showed approximately 30% degradation of straw residue after 10 days incubation.

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Bioethanol production from wheat straw via enzymatic route employing Penicillium janthinellum cellulases

Cited by 93 publications

References 32 publications

Open Fermentative Production of L-Lactic Acid from Distillers’ Grains by Lactobacillus casei CICC 6056

Open Fermentative Production of L-Lactic Acid from Distillers’ Grains by Lactobacillus casei CICC 6056

Improved saccharification of pilot-scale acid pretreated wheat straw by exploiting the synergistic behavior of lignocellulose degrading enzymes

Crop Residue Degradation by Autochthonous Fungi Isolated from Cropping System Management Scenarios

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