Bioethanol production from taro waste using thermo-tolerant yeast Kluyveromyces marxianus K21

Wu, Wei-Hao; Hung, Wei‐Chun; Lo, Kai-Yin; Chen, Yen‐Hui; Wan, Hou‐Peng; Cheng, Kuan‐Chen

doi:10.1016/j.biortech.2015.11.015

Cited by 70 publications

(29 citation statements)

References 30 publications

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“…The capacity of K. marxianus to utilize a wide variety of sugars reflects its potential for biotechnological applications [29,84], which has been indicated by many studies with diverse substrates such as whey permeate, crop plants, and lignocellulosic biomass [32,33,78,85,86].…”

Section: Complete Genome Sequence Of Thermotolerant Yeast K Marxianumentioning

confidence: 99%

Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Kosaka¹,

Lertwattanasakul²,

NadchanokRodrussamee³

et al. 2019

Fuel Ethanol Production From Sugarcane

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Thermotolerant ethanologenic yeasts receive attention as alternative bio-ethanol producers to traditionally used yeast, Saccharomyces cerevisiae. Their utilization is expected to provide several benefits for bio-ethanol production due to their characteristics and robustness. They have been isolated from a wide variety of environments in a number of ASEAN countries: Thailand, Vietnam, Laos, and Indonesia. One of these yeasts, Kluyveromyces marxianus has been investigated regarding characteristics. Some strains efficiently utilize xylose, which is a main component of the 2nd generation biomass. In addition, the genetic basis of K. marxianus has been revealed by genomic sequencing and is exploited for further improvement of the strains by thermal adaptation or gene engineering techniques. Moreover, the glucose repression of K. marxianus and its mechanisms has been investigated. Results suggest that K. marxianus is an alternative to S. cerevisiae in next-generation bio-ethanol production industry. Indeed, we have succeeded to apply K. marxianus for bio-ethanol production in a newly developed process, which combines high-temperature fermentation with simultaneous fermentation and distillation under low pressure. This chapter aims to provide valuable information on thermotolerant ethanologenic yeasts and their application, which may direct the economic bioproduction of ethanol and other useful materials in the future.Recently, thermotolerant microorganisms were found among mesophiles with optimum growth temperatures that are 5-10°C higher than those of the typical mesophilic strains Fuel Ethanol Production from Sugarcane 122 Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries… http://dx.doi.org/10.5772/intechopen.79144 123Recently, there have been several reports on ethanol production at high temperatures using P. kudriavzevii (formerly known as I. orientalis). Several P. kudriavzevii strains were reported to grow and produce high levels of ethanol at high temperatures. The strain DMKU 3-ET15 was isolated from traditional fermented pork sausage in Thailand by an enrichment technique in a medium supplemented with 4% ethanol at 40°C. The strain produced 78.6 g/L ethanol from 180 g/L glucose at 40°C [20]. The strain KVMP10 that was isolated from soil located beneath apple trees for ethanol production from orange peel achieved 54 g/L ethanol at 42°C [48]. Strain RZ8-1 that was recently isolated from various samples collected from plant orchards in Thailand produced 33.8 g/L ethanol from 160 g/L glucose at 40°C [49].

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Section: Complete Genome Sequence Of Thermotolerant Yeast K Marxianumentioning

confidence: 99%

Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Kosaka¹,

Lertwattanasakul²,

NadchanokRodrussamee³

et al. 2019

Fuel Ethanol Production From Sugarcane

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“…Yu-Sheng et al [73] studied the bioethanol production using a K. marxianus thermotolerant yeast, through the SSF process in a rotating reactor, which allowed a constant exchange of biomass that was in contact with the yeast, concluding that through this process bioethanol production has commercial potential. Wu et al [84] implemented a SSF process with a high solid load of taro waste using K. marxianus, reaching 94% of theoretical yields in 20 h of fermentation, which was reflected in high process productivity (Wu et al [84]).…”

Section: K Marxianus In the Bioethanol Production Through Ssfmentioning

confidence: 99%

“…It is estimated that 859,000 tons of agave are Table 2. Second-generation bioethanol production using K. marxianus thermotolerant yeasts in SSF processes [26,50,[68][69][70][71][72][73][74][75][76][77][78][79][80][81][82][83][84].…”

Section: Substrate Selection For Second-generation Bioethanol Productmentioning

confidence: 99%

Second-Generation Bioethanol Production through a Simultaneous Saccharification-Fermentation Process Using Kluyveromyces Marxianus Thermotolerant Yeast

Mejía-Barajas¹,

Alvarez-Navarrete²,

AlfredoSaavedra-Molina³

et al. 2018

Special Topics in Renewable Energy Systems

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Due to the present renewable fuels demand increase, reduction of second-generation bioethanol production cost is pursued, since it is considered the most promising biofuel, but not yet economically viable. A proposed solution is its production through a simultaneous saccharification and fermentation process (SSF); however, it is necessary to apply temperatures above 40°C, which reduce the viability of traditional ethanologenic yeasts. As consequence, the use of thermotolerant ethanologenic yeast has been suggested, among which the yeast Kluyveromyces marxianus stands out. This chapter addresses the production of second-generation bioethanol through the SSF process, emphasizing the potential of K. marxianus to transform lignocellulosic biomass as agave bagasse. As result, it is proposed to direct the second-generation bioethanol production to the SSF process employing thermotolerant yeasts, to increase process productivity, and addressing the economic barriers.

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“…A general screening method is based on targeted gene overexpression in host cells ( e.g., Escherichia coli or yeast). The overexpressed enzyme candidate was purified through several stages of liquid chromatography separations for further characterization and enzymatic activity analysis [3–5]. This approach can be readily incorporated into protein structure analysis and provide insights in protein activities.…”

Section: Introductionmentioning

confidence: 99%

Finding Biomass Degrading Enzymes Through an Activity-Correlated Quantitative Proteomics Platform (ACPP)

Delafield

Wang

You

et al. 2017

J. Am. Soc. Mass Spectrom.

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The microbial secretome, known as a pool of biomass (i.e., plant-based materials) degrading enzymes, can be utilized to discover industrial enzyme candidates for biofuel production. Proteomics approaches have been applied to discover novel enzyme candidates through comparing protein expression profiles with the whole secretome enzymatic activity under different growth conditions. However, the activity measurement of each enzyme candidate is needed for confident “active” enzyme assignments, and it remains to be elucidated. To address this challenge, we have developed an Activity-Correlated Quantitative Proteomics Platform (ACPP) that systematically correlate protein-level enzymatic activity patterns and protein elution profiles using a label-free quantitative proteomics approach. The ACPP optimized a high performance anion exchange separation for efficiently fractionating complex protein samples while preserving enzymatic activities. The detected enzymatic activity patterns in sequential fractions using microplate-based assays were cross-correlated with protein elution profiles using a customized pattern-matching algorithm with a correlation R score. The ACPP has been successfully applied to the identification of two types of “active” biomass-degrading enzymes (i.e., starch hydrolysis enzymes and cellulose hydrolysis enzymes) from Aspergillus niger secretome in a multiplexed fashion. By determining protein elution profiles of 156 proteins in A. niger secretome, we confidently identified the 1, 4-α-glucosidase as the major “active” starch hydrolysis enzyme (R=0.96) and the endoglucanase as the major “active” cellulose hydrolysis enzyme (R=0.97). The results demonstrated that the ACPP facilitated the discovery of bioactive enzymes from complex protein samples in a high-throughput, multiplexing, and untargeted fashion.

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Bioethanol production from taro waste using thermo-tolerant yeast Kluyveromyces marxianus K21

Cited by 70 publications

References 30 publications

Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Second-Generation Bioethanol Production through a Simultaneous Saccharification-Fermentation Process Using Kluyveromyces Marxianus Thermotolerant Yeast

Finding Biomass Degrading Enzymes Through an Activity-Correlated Quantitative Proteomics Platform (ACPP)

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