“…The polarity of extraction solvent affect the composition of phytochemicals in the crude extract (Abdel-Aal et al, 2015;Awotedu et al, 2020) and methanol is a good extraction solvent to isolate phytochemicals (Abdel-Aal et al, 2015; Rajkumar and Bhavan, 2017; Ramarajan and Janakiraman, 2019).The same way this study also observed that majority of phytochemicals were extracted by the methanol, and belong to alkaloids, anthraquinones, flavonoids, glycosides, lignin, phenols, proteins, quinones, reducing sugars, saponins, steroids, terpenoids and tannins classes (Table 1). Similar result was also reported by many workers (Yokota et al, 1987;Ghazala and Shameel, 2005;Nailwal et al, 2013;Stirk et al, 2013) that methanolic extract of H. reticulatum can efficiently extract various phytochemicals including steroids, terpenoids, carbohydrates and fatty acids, and relatively less effective in case of lignin and resin contents (Kim et al, 2017). The earlier studies (Sunilson et al, 2009;Janarthanan and Kumar, 2013;Shiney et al, 2014;Leelavathi and Prasad, 2015) reported the presence of many secondary metabolites in green algae say in Spirogyra longata which possess ninety seven compounds.…”