(2015) Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells. Cytotherapy, 17 (12). pp. 1706 -1722 . ISSN 1477 -2566 Access from the University of Nottingham repository: http://eprints.nottingham.ac.uk/31287/1/Submission%20Manuscript.pdf
Copyright and reuse:The Nottingham ePrints service makes this work by researchers of the University of Nottingham available open access under the following conditions. This article is made available under the University of Nottingham End User licence and may be reused according to the conditions of the licence. For more details see: http://eprints.nottingham.ac.uk/end_user_agreement.pdf
A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. However, the optimal culture conditions are disputed and few direct media comparisons have been performed. In this report, we evaluated several media types to identify the optimal for inducing an in vitro stem cell phenotype. Discussion: Culture medium can significantly influence CSSC phenotype. SCM produced a cell phenotype closest to that of a pluripotent stem cell, and we considerate to be the most appropriate for development as a clinical grade medium for the production of CSSC phenotypes suitable for cell therapy.
Methods