Bioengineering Organized, Multilamellar Human Corneal Stromal Tissue by Growth Factor Supplementation on Highly Aligned Synthetic Substrates

Wu, Jinhui; Du, Yiqin; Mann, Mary M.; Yang, Eun‐Ju; Funderburgh, James L.; Wagner, William R.

doi:10.1089/ten.tea.2012.0545

Cited by 100 publications

(120 citation statements)

References 45 publications

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“…After the amplification, cells are switched to a serum-free keratocyte-differentiation medium (Advanced DMEM supplemented with ascorbic acid and FGF-2). Similar to what was reported with the corneal fibroblasts, adding TGF-3 to the growth medium also increased the thickness of these stromal substitutes (Wu et al, 2013). Combining FGF-2 and TGF-3 to the growth medium generated tissues of up to 70-µm thickness (after 9 weeks).…”

Section: Limbal Stem Cellssupporting

confidence: 79%

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Alternatives to eye bank native tissue for corneal stromal replacement

Brunette

Roberts

Vidal

et al. 2017

Progress in Retinal and Eye Research

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Section: Limbal Stem Cellssupporting

confidence: 79%

“…To produce stromal substitutes, the stromal stem cells isolated from stromal tissue taken at the limbus of eye bank corneas (Wu et al, 2014;Wu et al, 2013) or from patients' limbal biopsies (Basu et al, 2014) are first amplified in a stem growth medium (described in 13. Table 3.1) supplemented with cholera toxin.…”

Section: Limbal Stem Cellsmentioning

confidence: 99%

Alternatives to eye bank native tissue for corneal stromal replacement

Brunette

Roberts

Vidal

et al. 2017

Progress in Retinal and Eye Research

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“…SCM also contains the recombinant proteins bFGF and LIF. bFGF is associated with differentiation to the keratocyte phenotype [57] and is essential for the maintenance of pluripotency of hESC [58]. LIF is a factor that is essential in the maintenance of pluripotency in mouse ESC [59] but has also been identified as important in naïve hESC [60].…”

Section: Discussionmentioning

confidence: 99%

Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells

et al. 2015

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(2015) Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells. Cytotherapy, 17 (12). pp. 1706 -1722 . ISSN 1477 -2566 Access from the University of Nottingham repository: http://eprints.nottingham.ac.uk/31287/1/Submission%20Manuscript.pdf Copyright and reuse:The Nottingham ePrints service makes this work by researchers of the University of Nottingham available open access under the following conditions. This article is made available under the University of Nottingham End User licence and may be reused according to the conditions of the licence. For more details see: http://eprints.nottingham.ac.uk/end_user_agreement.pdf A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. However, the optimal culture conditions are disputed and few direct media comparisons have been performed. In this report, we evaluated several media types to identify the optimal for inducing an in vitro stem cell phenotype. Discussion: Culture medium can significantly influence CSSC phenotype. SCM produced a cell phenotype closest to that of a pluripotent stem cell, and we considerate to be the most appropriate for development as a clinical grade medium for the production of CSSC phenotypes suitable for cell therapy. Methods

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“…32,34,[88][89][90] Human stromal stem cells have been used to repopulate mouse corneas and restore stromal thickness, fibril deficits, and transparency in cloudy corneas found in lumican -/ -mice, 91 and in natural and synthetic polymers-based in vitro systems. 58,67 The cells were found to be stably integrated into the mouse cornea for > 10 weeks. Importantly, the stem cells did not elicit an immune response, while under the same conditions corneal fibroblasts were immunogenic.…”

Section: Cell Sourcesmentioning

confidence: 99%

“…58 The combination of synthetic polymers together with natural-derived materials provides enhanced biological responses and more amenable optical properties. 59 As an example, hydrogels films prepared from chitosan blended with poly(ethylene glycol) and poly (l,d lactic acid) nanofibers in a composite with type I collagen hydrogel displayed improved mechanical, optical, and biological performances compared with synthetic fibers alone.…”

mentioning

confidence: 99%