Bioengineering of the silica-polymerizing enzyme silicatein-α for a targeted application to hydroxyapatite

Natálio, Filipe; Link, Thorben; Müller, Wernér E.G.; Schröder, Heinz C.; Cui, Fuzhai; Wang, Xiaohong; Wiens, Matthias

doi:10.1016/j.actbio.2010.03.010

Cited by 43 publications

(33 citation statements)

References 57 publications

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“…These types of surfaces are of high interest for the tissue engineering field since they can be used to promote new bone formation [69]. This process can now be performed under physiologically relevant conditions [70]. This was achieved with the modification of the protein with a sequence that renders it binding affinity to hydroxyapatite (HA).…”

Section: Surface Modification For Medical Applicationsmentioning

confidence: 99%

“…Due to these properties, this bioengineered silicatein presents important biomedical potential in the development of novel bioactive bone replacement materials or in the regeneration of bone defects. In addition, tooth surface defects and dentinal tubules could be corrected by the use of immobilized silicatein-synthesized biosilica layers [70].…”

Section: Surface Modification For Medical Applicationsmentioning

confidence: 99%

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Biosilica – Nanocomposites – Nanobiomaterials for Biomedical Engineering and Sensing Applications

Chaniotakis

Buiculescu

2012

Biomedical Materials and Diagnostic Devices

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Biosilicification is a process by which silica-based nanostructures are formed (biosilica) through a biopolymer-based catalysis. Found in nature, biosilica is produced by many organisms and is renowned for its elaborate shapes down to nanoscale size. Biosilica is physically very strong, biocompatible, and has unique optical and chemical properties. Its surface is covered with chemically functional groups which are ideal for functionalization, protein adsorption and enzyme stabilization. Biomolecules entrapped within biosilica are highly stabilized from chemical denaturation, while protected from protease attacks. The ability to synthesize biosilica in the laboratory has set the grounds for the beginning of a new technological era which will provide us with novel devices and tools applicable to a variety of technologies. This chapter is devoted to the discussion of the engineering applications of biosilica in the biomedical and sensing areas.

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Section: Surface Modification For Medical Applicationsmentioning

confidence: 99%

Section: Surface Modification For Medical Applicationsmentioning

confidence: 99%

Biosilica – Nanocomposites – Nanobiomaterials for Biomedical Engineering and Sensing Applications

Chaniotakis

Buiculescu

2012

Biomedical Materials and Diagnostic Devices

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“…Since then, silicatein and its silicate substrate have been applied in vivo in a poly(D,L-lactide)/ poly(vinyl pyrrolidone)-based matrix as a prototypic implant material [20]. Concurrently, genetic modification of silicatein has extended the spectrum of possible biomedical application of biosilica: silicatein was fused to a biomimetic protein tag (Glutag) that conferred HA binding affinity and, hence, facilitated the coating of HA substrates with biosilica [21]. Finally, a new polyurethane dimethacrylate/methacryloxypropyltrimethoxysilane (PUD-MA-co-MPS) copolymer was synthesized and functionalized with biosilica through the polycondensation activity of immobilized silicatein to obtain an osteogenic surface coating, using both soluble orthosilicate as well as silanol groups on the copolymer surface as enzyme substrate [22].…”

Section: Introductionmentioning

confidence: 99%

Characterization and osteogenic activity of a silicatein/biosilica-coated chitosan-graft-polycaprolactone

et al. 2014

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“…The cDNAs, SDNOSIP-r and SDGNB-Trans, were amplified by standard polymerase chain reaction (PCR) as described (Natalio et al 2010). …”

Section: Preparation Of Recombinant Nosip and Tblr Protein And Antibomentioning

confidence: 99%

Cryptochrome in Sponges

Müller

Schröder

Markl

et al. 2013

J Histochem Cytochem.

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SummarySponges (phylum: Porifera) react to external light or mechanical signals with contractile or metabolic reactions and are devoid of any nervous or muscular system. Furthermore, elements of a photoreception/phototransduction system exist in those animals. Recently, a cryptochrome-based photoreceptor system has been discovered in the demosponge. The assumption that in sponges the siliceous skeleton acts as a substitution for the lack of a nervous system and allows light signals to be transmitted through its glass fiber network is supported by the findings that the first spicules are efficient light waveguides and the second sponges have the enzymatic machinery for the generation of light. Now, we have identified/ cloned in Suberites domuncula two additional potential molecules of the sponge cryptochrome photoreception system, the guanine nucleotide-binding protein β subunit, related to β-transducin, and the nitric oxide synthase (NOS)-interacting protein. Cryptochrome and NOSIP are light-inducible genes. The studies show that the NOS inhibitor L-NMMA impairs both morphogenesis and motility of the cells. Finally, we report that the function of primmorphs to produce reactive nitrogen species can be abolished by a NOS inhibitor. We propose that the sponge cryptochrome-based photoreception system, through which photon signals are converted into radicals, is coupled to the NOS apparatus. ( J Histochem Cytochem 61:814-832, 2013)

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Bioengineering of the silica-polymerizing enzyme silicatein-α for a targeted application to hydroxyapatite

Cited by 43 publications

References 57 publications

Biosilica – Nanocomposites – Nanobiomaterials for Biomedical Engineering and Sensing Applications

Biosilica – Nanocomposites – Nanobiomaterials for Biomedical Engineering and Sensing Applications

Characterization and osteogenic activity of a silicatein/biosilica-coated chitosan-graft-polycaprolactone

Cryptochrome in Sponges

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