Bioengineering of a functional sheet of islet cells for the treatment of diabetes mellitus

Shimizu, Hirofumi; Ohashi, Kazuhiko; Utoh, Rie; Ise, Kazuya; Gotoh, Mitsukazu; Yamato, Masayuki; Okano, Teruo

doi:10.1016/j.biomaterials.2009.07.042

Cited by 116 publications

(88 citation statements)

References 40 publications

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“…Second, detaching cell sheets from the membrane requires careful treatment and is a time-consuming process. Other materials have also been used recently; 7,8 however, improvements in the flexibility and rapidity of cell sheet detachment are still required.…”

Section: Development Of a Novel Carrier Optimized For Cell Sheet Tranmentioning

confidence: 99%

Development of a novel carrier optimized for cell sheet transplantation

Amagai¹,

Karasawa²,

Jung³

et al. 2015

Biomatter

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Section: Development Of a Novel Carrier Optimized For Cell Sheet Tranmentioning

confidence: 99%

Development of a novel carrier optimized for cell sheet transplantation

Amagai¹,

Karasawa²,

Jung³

et al. 2015

Biomatter

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“…Once thought to be a simple scaffold, the ECM has recently being shown to regulate several aspects of islet biology, including development, morphology and differentiation, intracellular signaling, gene expression, adhesion and migration, proliferation, secretion, and survival. The ECM mediates these functions via three-dimensional structure (116,117), signaling molecules, and the secretion and storage of growth factors and cytokines (118). The ECM is therefore essential for effective glucose-stimulated insulin secretion and normoglycemia, the primary function of the endocrine pancreas.…”

Section: Ecm and Endocrine Pancreas Bioengineeringmentioning

confidence: 99%

“…The functional significance of the ECM has been highlighted by the underperformance of isolated islet transplantation (117,(119)(120)(121)(122). The seminal Edmonton protocol (123) attempted to correct hyperglycemia through the transplantation of isolated islets from multiple cadaveric donors.…”

Section: Ecm and Endocrine Pancreas Bioengineeringmentioning

confidence: 99%

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Cell Replacement Strategies Aimed at Reconstitution of the β-Cell Compartment in Type 1 Diabetes

et al. 2014

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Emerging technologies in regenerative medicine have the potential to restore the b-cell compartment in diabetic patients, thereby overcoming the inadequacies of current treatment strategies and organ supply. Novel approaches include: 1) Encapsulation technology that protects islet transplants from host immune surveillance; 2) stem cell therapies and cellular reprogramming, which seek to regenerate the depleted b-cell compartment; and 3) whole-organ bioengineering, which capitalizes on the innate properties of the pancreas extracellular matrix to drive cellular repopulation. Collaborative efforts across these subfields of regenerative medicine seek to ultimately produce a bioengineered pancreas capable of restoring endocrine function in patients with insulin-dependent diabetes.Regenerative medicine promises to contribute to the advancement of b-cell replacement strategies through the development and implementation of microencapsulation technology, the production of insulin-producing cells either by regeneration from endogenous cells or reprogramming from nonendocrine adult cell sources, and, more recently, the exploitation of bioengineered microenvironments (1). Considering the shortage of available pancreata, the search for alternative cell sources has recently been categorized within one of the following three "Rs" of pancreatic b-cell replenishment: replacement (from stem cells or xenoislets), regeneration (from endogenous progenitor cells), and reprogramming (from nonendocrine adult cell sources) (2).The purpose of this article is to comprehensively and critically review the main regenerative medicine2based strategies that are currently being developed to treat type 1 diabetes. The first part of the article will illustrate and discuss islet encapsulation technology, which has been extensively investigated over the past 40 years (3) and represents one of the most promising regenerative medicine2based approaches to achieve immunoisolation of transplantable organs. The second part will focus on cell bioengineering, where different types of progenitor and differentiated cell sources are manipulated to ultimately yield insulin-producing cells. The last part of the article will summarize the most recent advancements in the study of the pancreatic extracellular matrix (ECM) as a platform for endocrine pancreas bioengineering. ISLET ENCAPSULATION TECHNOLOGYEncapsulation technology has been used to protect islets from the host immune system. This strategy holds the promise to allow implantation of islets without immunosuppression not only across genetically different individuals from the same species, but also across species barriers. MicroencapsulationThe first approach to immunoisolate cells consists of the microencapsulation of one to three islets per semipermeable immunoprotective capsule. The spherical configuration of these microcapsules results in a higher surface-tovolume ratio and a higher diffusion rate (4). Furthermore, microcapsules can be injected in large numbers, are durable, and are difficult to disrup...

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“…Other applications using cell sheet engineering, such as liver [53], pancreatic islet [54], and bladder augmentation [55], have been investigated and summarized in other reviews [24][25][26]. Recent progress on the next generation of thermoresponsive culture dishes is discussed in the next section.…”

Section: Cell Sheet-based Tissue Engineering and Its Clinical Applicamentioning

confidence: 99%

Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering

Kobayashi¹,

Okano²

2010

Science and Technology of Advanced Materials

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This article reviews the properties and characterization of an intelligent thermoresponsive surface, which is a key technology for cell sheet-based tissue engineering. Intelligent thermoresponsive surfaces grafted with poly(N-isopropylacrylamide) exhibit hydrophilic/hydrophobic alteration in response to temperature change. Cultured cells are harvested on thermoresponsive cell culture dishes by decreasing the temperature without the use of digestive enzymes or chelating agents. Our group has developed cell sheet-based tissue engineering for therapeutic uses with single layer or multilayered cell sheets, which were recovered from the thermoresponsive cell culture dish. Using surface derivation techniques, we developed a new generation of thermoresponsive cell culture dishes to improve culture conditions. We also designed a new methodology for constructing well-defined organs using microfabrication techniques.

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Bioengineering of a functional sheet of islet cells for the treatment of diabetes mellitus

Cited by 116 publications

References 40 publications

Development of a novel carrier optimized for cell sheet transplantation

Development of a novel carrier optimized for cell sheet transplantation

Cell Replacement Strategies Aimed at Reconstitution of the β-Cell Compartment in Type 1 Diabetes

Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering

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