Bioengineering Microgels and Hydrogel Microparticles for Sensing Biomolecular Targets

Battista, Edmondo; Causa, Filippo; Netti, Paolo Antonio

doi:10.3390/gels3020020

Cited by 23 publications

(14 citation statements)

References 111 publications

(153 reference statements)

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“…Preliminary studies for the detection assay optimization were performed using a probe scheme already tested in previous work by our group [ 55 , 56 , 57 , 58 ]. An overview of probe mechanism is reported in Scheme 1 (lower part), where fluorescent T-DNA is a short fluorescent DNA tail (12 nt), labelled at 5′ end with ATTO 647N and modified at 3′ with methacrylamide spacer to allow its covalent binding with polymer network.…”

Section: Resultsmentioning

confidence: 99%

Small Oligonucleotides Detection in Three-Dimensional Polymer Network of DNA-PEG Hydrogels

Mazzarotta

Caputo

Raiola

et al. 2021

Gels

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The control of the three-dimensional (3D) polymer network structure is important for permselective materials when specific biomolecule detection is needed. Here we investigate conditions to obtain a tailored hydrogel network that combines both molecular filtering and molecular capture capabilities for biosensing applications. Along this line, short oligonucleotide detection in a displacement assay is set within PEGDA hydrogels synthetized by UV radical photopolymerization. To provide insights on the molecular filter capability, diffusion studies of several probes (sulforhodamine G and dextrans) with different hydrodynamic radii were carried out using NMR technique. Moreover, fluorometric analyses of hybridization of DNA oligonucleotides inside PEGDA hydrogels shed light on the mechanisms of recognition in 3D, highlighting that mesh size and crowding effect greatly impact the hybridization mechanism on a polymer network. Finally, we found the best probe density and diffusion transport conditions to allow the specific oligonucleotide capture and detection inside PEGDA hydrogels for oligonucleotide detection and the filtering out of higher molecular weight molecules.

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Section: Resultsmentioning

confidence: 99%

Small Oligonucleotides Detection in Three-Dimensional Polymer Network of DNA-PEG Hydrogels

Mazzarotta

Caputo

Raiola

et al. 2021

Gels

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“…To apply this equation, several parameters are needed: interaction parameter PEG/water, χ=0.426 43 ; specific volume, v=0.91 ml/g; molar volume of water, V1= 18.1 ml/mol 43 ; uncrosslinked molecular weight of PEG, Mn = 700 Da [48][49][50]. Polymer volume fraction in the swollen state (v2,s) characterizes how well the polymer absorbs water.…”

Section: Bulk-hydrogel Characterizationsmentioning

confidence: 99%

Small Oligonucleotides Detection in Three-Dimensional Polymer Network of DNA-PEG Hydrogels

Mazzarotta

Caputo

Raiola

et al. 2021

Preprint

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The control of the three-dimensional (3D) polymer network structure is important for permselective materials when specific biomolecules detection is needed. Here we investigate conditions to obtain a tailored hydrogel network that combine both molecular filtering and molecular capture capabilities for biosensing applications. Along this line short oligonucleotide detection in a displacement assay is set within PEGDA hydrogels synthetized by UV radical photopolymerization. To provide insights on the molecular filter capability, diffusion studies of several probes (sulforhodamine G and dextrans) with different hydrodynamic radii were carried out using NMR technique. Moreover, fluorometric analyses of hybridization of DNA oligonucleotides inside PEGDA-hydrogels shed light on the mechanisms of recognition in 3D, highlighting that mesh size and crowding effect greatly impact of hybridization mechanism onto polymer network. Finally, we found the best probe density and diffusion transport conditions to allow the specific oligonucleotide capture and detection inside PEGDA-hydrogels for oligonucleotide detection and the filtering out of higher molecular weight molecules.

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“…An antibody, with its selective binding ability against certain protein antigens, has conventionally been adapted to the enzyme-linked immunosorbent assay (ELISA) [73]. Incorporated into a hydrogel matrix platform, antibodies enable sensitive detection of proteins through liquid-phase ELISA [74]. For instance, capture antibodies targeting IL-2, IL-4, and TNF-α cytokines can be loaded onto PEGDA gel microparticles through the heterobifunctional PEG linker.…”

Section: Proteinsmentioning

confidence: 99%

Hydrogel-Based Technologies for the Diagnosis of Skin Pathology

et al. 2020

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Hydrogels, swellable hydrophilic polymer networks fabricated through chemical cross-linking or physical entanglement are increasingly utilized in various biomedical applications over the past few decades. Hydrogel-based microparticles, dressings and microneedle patches have been explored to achieve safe, sustained and on-demand therapeutic purposes toward numerous skin pathologies, through incorporation of stimuli-responsive moieties and therapeutic agents. More recently, these platforms are expanded to fulfill the diagnostic and monitoring role. Herein, the development of hydrogel technology to achieve diagnosis and monitoring of pathological skin conditions are highlighted, with proteins, nucleic acids, metabolites, and reactive species employed as target biomarkers, among others. The scope of this review includes the characteristics of hydrogel materials, its fabrication procedures, examples of diagnostic studies, as well as discussion pertaining clinical translation of hydrogel systems.

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Bioengineering Microgels and Hydrogel Microparticles for Sensing Biomolecular Targets

Cited by 23 publications

References 111 publications

Small Oligonucleotides Detection in Three-Dimensional Polymer Network of DNA-PEG Hydrogels

Small Oligonucleotides Detection in Three-Dimensional Polymer Network of DNA-PEG Hydrogels

Small Oligonucleotides Detection in Three-Dimensional Polymer Network of DNA-PEG Hydrogels

Hydrogel-Based Technologies for the Diagnosis of Skin Pathology

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