Bioengineering-inspired three-dimensional culture systems: Organoids to create tumor microenvironment

Sağlam-Metiner, Pelin; Gülce-İz, Sultan; Avci, Çığır Biray

doi:10.1016/j.gene.2018.11.058

Cited by 72 publications

(64 citation statements)

References 66 publications

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“…At the same time, the 3D cell culture model was developed as a new in vitro cell culture model. The cells cultured in 3D cell culture model exhibit features that are close to the complex in vivo conditions and have been proven realistic for translating the study findings for in vivo applications (Saglam‐Metiner, Gulce‐Iz, & Biray‐Avci, ; Swaminathan, Hamid, Sun, & Clyne, ; Wu et al., ). In this work, we used hydrogel built the 3D cell culture model, this method is inconvenient and time consuming.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and Antiproliferative Activities of Cyanidin‐3‐O‐Glucoside (C3G) Liposome in Caco‐2 Cells Cultivated in 2D and 3D Cell Culture Models

Liang

Qian

Guan

et al. 2019

Journal of Food Science

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The aim of this study was to obtain adequate and detailed information about the antioxidant and antiproliferative activities of C3G and C3G liposomes in different cell culture models. The Caco‐2 cells were cultured in 2D and 3D cell culture models, the H2O2 was used to construct the cell damage model and then the cells treated with C3G and C3G liposomes. The antioxidant activity and antiproliferative activities of C3G liposomes on Caco‐2 cells were investigated. We observed the morphology of cells and measured the cell viability, the activity of glutathione (GSH), superoxide dismutase (SOD), total antioxidant capacity (T‐AOC), and the content of malondialdehyde (MDA) in Caco‐2 cells treated with H2O2, C3G, and C3G liposomes. The results showed that the Caco‐2 cells cultured in the 3D culture model formed a 3D structure and tight spheroids and showed the increase of cell activity in 3D cell culture model, compared with the 2D cell culture model. The C3G and C3G liposomes can enhance the activities of GSH, SOD, and T‐AOC but decrease the MDA content after H2O2 treatment, while the changes were different in 2D and 3D cells culture models. This study revealed that the results obtained from the 2D cell model may be inaccurate compared with the results obtained from the 3D cell model. Practical Application The results of this study showed that the results obtained from the 2D cell model may be inaccurate compared with the results obtained from the 3D cell model. Our work provides a method for evaluating antioxidant activity of C3G liposomes in different cell models and provided certain theoretical basis for the follow‐up research.

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Section: Discussionmentioning

confidence: 99%

Antioxidant and Antiproliferative Activities of Cyanidin‐3‐O‐Glucoside (C3G) Liposome in Caco‐2 Cells Cultivated in 2D and 3D Cell Culture Models

Liang

Qian

Guan

et al. 2019

Journal of Food Science

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“…To our best knowledge, this study is the first one to use a 3D platform to culture a cohort of AML bone marrow samples ex vivo and profile gene signatures and genetic mutations that correlate with growth and Ara‐C responsiveness. 3D culture is currently used as a translational platform for drug discovery because of its physiological relevance and better prediction of drug efficacy . Extensive characterization of differentiation of major lineages of blood and stromal cells using bone marrow samples derived from healthy donors demonstrates that the ex vivo 3D platform appropriately mimics the bone marrow microenvironment.…”

Section: Discussionmentioning

confidence: 99%

“…7,8 To overcome this limitation, 3D culture models have been developed in recent years for various cell types and tissues. 7,8 The 3D ex vivo models can recapitulate the complex physiology and maintain functional in vivo responses that are not observed in routine 2D cultures. In the case of 2D culture of leukaemic BMMCs, lack of bone marrow microenvironment prevents long-term culture of leukaemic cells without supplementing maintenance media with growth factors or co-culture with stromal cells.…”

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confidence: 99%

Identification of predictive genetic signatures of Cytarabine responsiveness using a 3D acute myeloid leukaemia model

Muise

Javaid

et al. 2019

J Cellular Molecular Medi

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This study reports the establishment of a bone marrow mononuclear cell (BMMC) 3D culture model and the application of this model to define sensitivity and resistance biomarkers of acute myeloid leukaemia (AML) patient bone marrow samples in response to Cytarabine (Ara‐C) treatment. By mimicking physiological bone marrow microenvironment, the growth conditions were optimized by using frozen BMMCs derived from healthy donors. Healthy BMMCs are capable of differentiating into major hematopoietic lineages and various types of stromal cells in this platform. Cryopreserved BMMC samples from 49 AML patients were characterized for ex vivo growth and sensitivity to Ara‐C. RNA sequencing was performed for 3D and 2D cultures to determine differential gene expression patterns. Specific genetic mutations and/or gene expression signatures associated with the ability of the ex vivo expansion and response to Ara‐C were elucidated by whole‐exome and RNA sequencing. Data analysis identified unique gene expression signatures and novel genetic mutations associated with sensitivity to Ara‐C treatment of proliferating AML specimens and can be used as predictive therapeutic biomarkers to determine the optimal treatment regimens. Furthermore, these data demonstrate the translational value of this ex vivo platform which should be widely applicable to evaluate other therapies in AML.

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“…Since the development of the first 3D models, newer in vitro 3D cell culture systems, which simulate in vivo physiology and tissue microenvironment, have been developed and now play a crucial role in research. 3D models have been shown to recreate the natural microenvironment of tumor cells more accurately than 2D models in a relatively simple and cost‐effective way, and have been utilized in preclinical evaluations to test efficacy and safety profiles of potential pharmacotherapeutics against cancer . Cancer cells that proliferate in 3D culture systems display cell–cell and cell–matrix interactions, gene expression levels, signaling pathways, and structural integrity, which are more comparable to in vivo tumors than 2D models are .…”

Section: Introductionmentioning

confidence: 99%

“…3D models have been shown to recreate the natural microenvironment of tumor cells more accurately than 2D models in a relatively simple and cost-effective way, (32,(41)(42)(43) and have been utilized in preclinical evaluations to test efficacy and safety profiles of potential pharmacotherapeutics against cancer. (44) Cancer cells that proliferate in 3D culture systems display cell-cell and cell-matrix interactions, gene expression levels, signaling pathways, and structural integrity, which are more comparable to in vivo tumors than 2D models are. (45) Furthermore, 3D cultures demonstrate the features of solid tumors, including physiological responses and drug metabolism, more accurately than 2D systems can.…”

Section: Introductionmentioning

confidence: 99%

In Vitro 3D Cultures to Reproduce the Bone Marrow Niche

et al. 2019

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Over the past century, the study of biological processes in the human body has progressed from tissue culture on glass plates to complex 3D models of tissues, organs, and body systems. These dynamic 3D systems have allowed for more accurate recapitulation of human physiology and pathology, which has yielded a platform for disease study with a greater capacity to understand pathophysiology and to assess pharmaceutical treatments. Specifically, by increasing the accuracy with which the microenvironments of disease processes are modeled, the clinical manifestation of disease has been more accurately reproduced in vitro. The application of these models is crucial in all realms of medicine, but they find particular utility in diseases related to the complex bone marrow niche. Osteoblast, osteoclasts, bone marrow adipocytes, mesenchymal stem cells, and red and white blood cells represent some of cells that call the bone marrow microenvironment home. During states of malignant marrow disease, neoplastic cells migrate to and join this niche. These cancer cells both exploit and alter the niche to their benefit and to the patient's detriment. Malignant disease of the bone marrow, both primary and secondary, is a significant cause of morbidity and mortality today. Innovative study methods are necessary to improve patient outcomes. In this review, we discuss the evolution of 3D models and compare them to the preceding 2D models. With a specific focus on malignant bone marrow disease, we examine 3D models currently in use, their observed efficacy, and their potential in developing improved treatments and eventual cures. Finally, we comment on the aspects of 3D models that must be critically examined as systems continue to be optimized so that they can exert greater clinical impact in the future. © 2019 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.

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Bioengineering-inspired three-dimensional culture systems: Organoids to create tumor microenvironment

Cited by 72 publications

References 66 publications

Antioxidant and Antiproliferative Activities of Cyanidin‐3‐O‐Glucoside (C3G) Liposome in Caco‐2 Cells Cultivated in 2D and 3D Cell Culture Models

Antioxidant and Antiproliferative Activities of Cyanidin‐3‐O‐Glucoside (C3G) Liposome in Caco‐2 Cells Cultivated in 2D and 3D Cell Culture Models

Identification of predictive genetic signatures of Cytarabine responsiveness using a 3D acute myeloid leukaemia model

In Vitro 3D Cultures to Reproduce the Bone Marrow Niche

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