Bioengineering Human Pluripotent Stem Cell-Derived Retinal Organoids and Optic Vesicle-Containing Brain Organoids for Ocular Diseases

Arthur, Peggy; Muok, Laureana; Nathani, Aakash; Zeng, Eric Z.; Lee, Sun; Li, Yan; Singh, Mandip

doi:10.3390/cells11213429

Cited by 8 publications

(4 citation statements)

References 217 publications

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“…To achieve this goal, investigators have developed “in vitro” approaches simulating organ environments, including the heart, adipose tissue, and skin, among others. More recently, iPSC-derived organoids have been developed to simulate various tissue environments, including the brain which have been particularly illuminating [ 83 ] . Skin, the largest organ in the organism, has appeared as a fascinating puzzle to understand EV biology and their functions in homeostasis and disease [ 84 ] .…”

Section: Systems To Simulate Ev Signaling “In Vitro” and “In Vivo” - ...mentioning

confidence: 99%

Extracellular vesicles - on the cusp of a new language in the biological sciences

Raposo¹,

Stahl²

2023

Extracell Vesicles Circ Nucleic Acids

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Extracellular vesicles (EVs) play a key role both in physiological balance and homeostasis and in disease processes through their ability to participate in intercellular signaling and communication. An ever-expanding knowledge pool and a myriad of functional properties ascribed to EVs point to a new language of communication in biological systems that has opened a path for the discovery and implementation of novel diagnostic applications. EVs originate in the endosomal network and via non-random shedding from the plasma membrane by mechanisms that allow the packaging of functional cargoes, including proteins, lipids, and genetic materials. Deciphering the molecular mechanisms that govern packaging, secretion and targeted delivery of extracellular vesicle-borne cargo will be required to establish EVs as important signaling entities, especially when ascribing functional properties to a heterogeneous population of vesicles. Several molecular cascades operate within the endosomal network and at the plasma membrane that recognize and segregate cargos as a prelude to vesicle budding and release. EVs are transferred between cells and operate as vehicles in biological fluids within tissues and within the microenvironment where they are responsible for short- and long-range targeted information. In this review, we focus on the remarkable capacity of EVs to establish a dialogue between cells and within tissues, often operating in parallel to the endocrine system, we highlighting selected examples of past and recent studies on the functions of EVs in health and disease.

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Section: Systems To Simulate Ev Signaling “In Vitro” and “In Vivo” - ...mentioning

confidence: 99%

Extracellular vesicles - on the cusp of a new language in the biological sciences

Raposo¹,

Stahl²

2023

Extracell Vesicles Circ Nucleic Acids

View full text Add to dashboard Cite

show abstract

“…The retina can be dissociated to generate different cell cultures, such as: (i) mixed cultures; (ii) neuron-enriched cultures; (iii) purified as Müller glia cultures; and (iv) neurospheres [5,6]. Recently, functional platforms originated from stem cell organoids are being engineered to mitigate ocular diseases [7]. Neurogenesis and tissue development are widely described in the embryonic and mature retina [8].…”

Section: Introductionmentioning

confidence: 99%

The Healthy and Diseased Retina Seen through Neuron–Glia Interactions

Tempone,

Borges-Martins,

César

et al. 2024

IJMS

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The retina is the sensory tissue responsible for the first stages of visual processing, with a conserved anatomy and functional architecture among vertebrates. To date, retinal eye diseases, such as diabetic retinopathy, age-related macular degeneration, retinitis pigmentosa, glaucoma, and others, affect nearly 170 million people worldwide, resulting in vision loss and blindness. To tackle retinal disorders, the developing retina has been explored as a versatile model to study intercellular signaling, as it presents a broad neurochemical repertoire that has been approached in the last decades in terms of signaling and diseases. Retina, dissociated and arranged as typical cultures, as mixed or neuron- and glia-enriched, and/or organized as neurospheres and/or as organoids, are valuable to understand both neuronal and glial compartments, which have contributed to revealing roles and mechanisms between transmitter systems as well as antioxidants, trophic factors, and extracellular matrix proteins. Overall, contributions in understanding neurogenesis, tissue development, differentiation, connectivity, plasticity, and cell death are widely described. A complete access to the genome of several vertebrates, as well as the recent transcriptome at the single cell level at different stages of development, also anticipates future advances in providing cues to target blinding diseases or retinal dysfunctions.

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“…Retina can be dissociated to generate different cell cultures, such as: (i) mixed cultures, (ii) neuron-enriched cultures, (iii) purified as Müller glia cultures, and (iv) neurospheres [5,6]. Recently, functional platforms originated from stem cell 2 organoids are being engineered to mitigate ocular diseases [7]. Neurogenesis and tissue development is widely described in the embryonic and mature retina [8].…”

Section: Introductionmentioning

confidence: 99%

“…Dieterich, D. C.; Hodas, J. J.; Gouzer, G.; Shadrin, I. Y.; Ngo, J. T.; Triller, A.; Tirrell, D. A.; Schuman, E. M.,In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons. Nat Neurosci 2010, 13,(7), 897-905. 47.…”

mentioning

confidence: 99%

The Healthy and Diseased Retina Seen through Neuron-Glia Interactions

Tempone,

Borges-Martins,

César

et al. 2024

Preprint

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The retina is the sensory tissue responsible for the first stages of visual processing, with a conserved anatomy and functional architecture among vertebrates. To date, retinal eye diseases, such as diabetic retinopathy, age-related macular degeneration, retinitis pigmentosa, glaucoma and others, affect nearly 170 million people worldwide, resulting in vision loss and blindness. To tackle retinal disorders, the developing retina has been explored as a versatile model to study intercellular signaling, as it presents a broad neurochemical repertoire. Retina, dissociated and arranged as typical cultures, as mixed or neuron- and glia-enriched, and/or organized as neurospheres or as organoids are valuable to understand both neuronal and glial compartments which have contributed to reveal roles and mechanisms of transmitter systems as well as antioxidants, trophic factors, and extracellular matrix proteins. Overall, contributions in understanding neurogenesis, tissue development, differentiation, connectivity, plasticity, and cell death are widely described. A complete access to the genome of several vertebrates, as well the recent transcriptome at the single cell level at different stages of development also anticipates future advances in providing cues to target blinding diseases or retinal dysfunctions.

show abstract

Bioengineering Human Pluripotent Stem Cell-Derived Retinal Organoids and Optic Vesicle-Containing Brain Organoids for Ocular Diseases

Cited by 8 publications

References 217 publications

Extracellular vesicles - on the cusp of a new language in the biological sciences

Extracellular vesicles - on the cusp of a new language in the biological sciences

The Healthy and Diseased Retina Seen through Neuron–Glia Interactions

The Healthy and Diseased Retina Seen through Neuron-Glia Interactions

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