Bioengineering Hairy Roots: Phytoremediation, Secondary Metabolism, Molecular Pharming, Plant-Plant Interactions and Biofuels

Habibi, Peyman; de, Maria Fatima Grossi; Makhzoum, Abdullah; Malik, Sonia; Silva, André Luís Lopes da; Hefferon, Kathleen; Soccol, Carlos Ricardo

doi:10.1007/978-3-319-48006-0_7

Cited by 23 publications

(10 citation statements)

References 213 publications

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“…Plant molecular pharming is the term related to the ability of plant materials to produce therapeutic proteins (Habibi et al 2017). The emergence of molecular pharming as a reliable and novel production technology in recent decades is the result of years of work, opening new paths to minimizing the technical problems involved in yeast, bacterial, and mammalian platforms.…”

Section: Introductionmentioning

confidence: 99%

Optimization of inside and outside factors to improve recombinant protein yield in plant

Habibi

Prado

Pelegrini³

et al. 2017

Plant Cell Tiss Organ Cult

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an improvement of critical features, including transcription, translation and post-translational modifications, in plant cells could make plant systems a safe and economical alternative for biopharmaceutical production. Hence, in this review, the most recent advances influencing the upstream and downstream processes involved in recombinant protein accumulation in plant cells are described. We also discuss how plant systems are becoming the benchmark for the production of several biopharmaceuticals.Keywords Heterologous expression · Host systems · Post-translational modifications · Plant-based pharmaceuticals · Recombinant protein identification and characterization of a promising regulatory pathway for the large-scale production of biopharmaceuticals could strongly contribute to the benefits of this system (Fischer et al. 2012). Molecular pharming technology presents several advantages, including (a) the production of low-cost biomass; (b) end-products lacking human toxicity; (c) the accumulation of complex proteins with correct and proper folding; and (d) straightforward methods for protein purification (Moustafa et al. 2015). Furthermore, molecular pharming offers a flexible, scalable and diverse alternative method for producing new, Abstract The use of plant systems as factories for recombinant protein production became a prominent alternative for pharmaceutical industries due to their high potential for protein accumulation. In the last decades, the application of plants for protein production has gained more attention, as plants represent an economic strategy that leads to high levels of purified and active proteins for the pharmaceutical sector. Currently, FDA approval of the first generation of recombinant proteins produced in carrot cells, taliglucerase alfa, demonstrated that plant cells have a significant capacity to express complex proteins for therapeutic use. Although plant systems still have technical and economic barriers that require improvements in future years, the optimization of upstream and downstream components affecting protein accumulation is considered a key feature in the development of new pharmaceutical proteins. Therefore,

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Section: Introductionmentioning

confidence: 99%

Optimization of inside and outside factors to improve recombinant protein yield in plant

Habibi

Prado

Pelegrini³

et al. 2017

Plant Cell Tiss Organ Cult

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“…Roots are regarded as a part of great secondary metabolism in the whole plant because these are a good source for bioactive molecules, proteins, and a range of metabolites [ 10 ]. Recent advances in plant biotechnology enable us to culture plant cells, tissues, and organs instead of complete plants to produce valuable secondary metabolites [ 11 ].…”

Section: Adventitious Root Culturementioning

confidence: 99%

Root Cultures, a Boon for the Production of Valuable Compounds: A Comparative Review

Hussain

Abbas

Nazli

et al. 2022

Plants

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Medicinal plants are an inevitable source of pharmaceutical drugs and most of the world population depends on these plants for health benefits. The increasing global demand for bioactive compounds from medicinal plants has posed a great threat to their existence due to overexploitation. Adventitious root and hairy root culture systems are an alternative approach to the conventional method for mass production of valuable compounds from medicinal plants owing to their rapid growth, biosynthetic and genetic stability. The main purpose of this review is to investigate the recent scientific research published worldwide on the application of adventitious and hairy root cultures to produce valuable compounds from medicinal plants. Furthermore, a comparison of adventitious root vs. hairy root cultures to produce valuable compounds has also been discussed. Various aspects such as medium composition, carbon source, pH, amount of macronutrients, optimization strategy, scale-up cultures, and use of biotic abiotic and nano-elicitors at various concentrations are the topic of discussion in this review. Several studies on adventitious and hairy root cultures of Polygonum multiflorum¸ Withania somnifera¸ Echinacea purpurea and Ajuga bracteosa have been discussed in detail which highlights the importance of elicitation strategies and bioreactor system, presenting commercial applications.

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“…In this context, plant cell cultures have emerged as sustainable and controllable technology for commercial production of active molecules. Advances in cell line selection, medium optimization, biotransformation, feeding precursors, hairy root cultures, product secretion, cell permeabilization and scale‐up increased plant natural products yield (Habibi et al ., 2017a, 2018a). The development of efficient, strong and novel ‘omics’ technologies such as next‐generation sequencing intensified plant cell culture system and in this regard the number of companies using plant cell cultures for the production of active natural products continues to rapidly expand (Ochoa‐Villarreal et al ., ).…”

Section: Current Approaches Towards Production Of Anti‐hiv and Tb Agementioning

confidence: 99%

The potential of plant systems to break the HIV‐TB link

Habibi

Daniell

Soccol

et al. 2019

Plant Biotechnology Journal

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Summary Tuberculosis (TB) and human immunodeficiency virus (HIV) can place a major burden on healthcare systems and constitute the main challenges of diagnostic and therapeutic programmes. Infection with HIV is the most common cause of Mycobacterium tuberculosis (Mtb), which can accelerate the risk of latent TB reactivation by 20‐fold. Similarly, TB is considered the most relevant factor predisposing individuals to HIV infection. Thus, both pathogens can augment one another in a synergetic manner, accelerating the failure of immunological functions and resulting in subsequent death in the absence of treatment. Synergistic approaches involving the treatment of HIV as a tool to combat TB and vice versa are thus required in regions with a high burden of HIV and TB infection. In this context, plant systems are considered a promising approach for combatting HIV and TB in a resource‐limited setting because plant‐made drugs can be produced efficiently and inexpensively in developing countries and could be shared by the available agricultural infrastructure without the expensive requirement needed for cold chain storage and transportation. Moreover, the use of natural products from medicinal plants can eliminate the concerns associated with antiretroviral therapy (ART) and anti‐TB therapy (ATT), including drug interactions, drug‐related toxicity and multidrug resistance. In this review, we highlight the potential of plant system as a promising approach for the production of relevant pharmaceuticals for HIV and TB treatment. However, in the cases of HIV and TB, none of the plant‐made pharmaceuticals have been approved for clinical use. Limitations in reaching these goals are discussed.

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Bioengineering Hairy Roots: Phytoremediation, Secondary Metabolism, Molecular Pharming, Plant-Plant Interactions and Biofuels

Cited by 23 publications

References 213 publications

Optimization of inside and outside factors to improve recombinant protein yield in plant

Optimization of inside and outside factors to improve recombinant protein yield in plant

Root Cultures, a Boon for the Production of Valuable Compounds: A Comparative Review

The potential of plant systems to break the HIV‐TB link

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