Bioengineering Fungi and Yeast for the Production of Enzymes, Metabolites, and Value-Added Compounds

Villena, Gretty K.; Kitazono, Ana A.; Hernández-Macedo, María Lucila

doi:10.1007/978-3-030-41870-0_9

Cited by 4 publications

(6 citation statements)

References 143 publications

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“…This is achieved by the transfer of enzymes or pathway reconstitution in a heterologous system, to achieve maximum results. Other gene editing methods, namely, DNA recombinant technology, CRISPR/Cas9, and RNA interference, alone or in combination, facilitate the production of value-added compounds including flavonoids, terpenoids, non-ribosomal peptides, alkaloids, biofuels, polymers, and enzymes, and others [ 77 ]. In this direction, attempts towards metabolic engineering of biological organisms should focus on the optimization of bioprocess technologies and parameters to obtain the yield of the desired products.…”

Section: Fungi—an Emerging Biological Platform For Metabolic Engineeringmentioning

confidence: 99%

Focus and Insights into the Synthetic Biology-Mediated Chassis of Economically Important Fungi for the Production of High-Value Metabolites

Tiwari

Dufossé²

2023

Microorganisms

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Substantial progress has been achieved and knowledge gaps addressed in synthetic biology-mediated engineering of biological organisms to produce high-value metabolites. Bio-based products from fungi are extensively explored in the present era, attributed to their emerging importance in the industrial sector, healthcare, and food applications. The edible group of fungi and multiple fungal strains defines attractive biological resources for high-value metabolites comprising food additives, pigments, dyes, industrial chemicals, and antibiotics, including other compounds. In this direction, synthetic biology-mediated genetic chassis of fungal strains to enhance/add value to novel chemical entities of biological origin is opening new avenues in fungal biotechnology. While substantial success has been achieved in the genetic manipulation of economically viable fungi (including Saccharomyces cerevisiae) in the production of metabolites of socio-economic relevance, knowledge gaps/obstacles in fungal biology and engineering need to be remedied for complete exploitation of valuable fungal strains. Herein, the thematic article discusses the novel attributes of bio-based products from fungi and the creation of high-value engineered fungal strains to promote yield, bio-functionality, and value-addition of the metabolites of socio-economic value. Efforts have been made to discuss the existing limitations in fungal chassis and how the advances in synthetic biology provide a plausible solution.

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Section: Fungi—an Emerging Biological Platform For Metabolic Engineeringmentioning

confidence: 99%

Focus and Insights into the Synthetic Biology-Mediated Chassis of Economically Important Fungi for the Production of High-Value Metabolites

Tiwari

Dufossé²

2023

Microorganisms

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“…This type of analysis has already been successfully used in multiple fields, from biomedical research 84,85 to biotechnology 86,87 . Furthermore, it has been demonstrated that in-silico modeling of complex biological networks can be a powerful tool for driving the improvement of commercially relevant organisms, such as the development of an improved model of Aspergillus nidulans metabolic network models, which is important for the construction and optimization of glucoamylase-producing strains [88][89][90] .…”

Section: Assembly Of a Multi-omics Network Model For Pca Responsementioning

confidence: 99%

Multi-omics network model reveals key genes associated with p-coumaric acid stress response in an industrial yeast strain

Ciamponi

Procópio

Murad

et al. 2022

Sci Rep

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The production of ethanol from lignocellulosic sources presents increasingly difficult issues for the global biofuel scenario, leading to increased production costs of current second-generation (2G) ethanol when compared to first-generation (1G) plants. Among the setbacks encountered in industrial processes, the presence of chemical inhibitors from pre-treatment processes severely hinders the potential of yeasts in producing ethanol at peak efficiency. However, some industrial yeast strains have, either naturally or artificially, higher tolerance levels to these compounds. Such is the case of S. cerevisiae SA-1, a Brazilian fuel ethanol industrial strain that has shown high resistance to inhibitors produced by the pre-treatment of cellulosic complexes. Our study focuses on the characterization of the transcriptomic and physiological impact of an inhibitor of this type, p-coumaric acid (pCA), on this strain under chemostat cultivation via RNAseq and quantitative physiological data. It was found that strain SA-1 tend to increase ethanol yield and production rate while decreasing biomass yield when exposed to pCA, in contrast to pCA-susceptible strains, which tend to decrease their ethanol yield and fermentation efficiency when exposed to this substance. This suggests increased metabolic activity linked to mitochondrial and peroxisomal processes. The transcriptomic analysis also revealed a plethora of differentially expressed genes located in co-expressed clusters that are associated with changes in biological pathways linked to biosynthetic and energetical processes. Furthermore, it was also identified 20 genes that act as interaction hubs for these clusters, while also having association with altered pathways and changes in metabolic outputs, potentially leading to the discovery of novel targets for metabolic engineering toward a more robust industrial yeast strain.

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“…Overall, tools such as genetic manipulation based on transcriptomic analysis, induction of mutations, cloning, and insertion of heterologous plasmids into species with well-known genomes have enabled the production of large quantities of metabolites by previously non-producing species [ 59 ]. The elucidation and manipulation of the different stages in the transcription and secretion of amylases, xylanases, and cellulases in filamentous fungi has enabled their overexpression [ 60 ]; these enzymes are widely used in bakery products to improve the quality of dough through hydrolysis of long-chain carbohydrates and non-starch polysaccharides (cellulose and arabinoxylans).…”

Section: Natural Food Additives From Fungimentioning

confidence: 99%

“…Many agro-industrial residues available all over the world are sources of peptides, prebiotic dietary fibers, and hydrolyzed or smaller organic molecules such as phenolics, carotenoids, and tocopherols, among other classes. These features make them good materials for fungal cultivation as well as interesting substrates for the production of bioactive components from macromolecules [ 59 , 136 ]. For example, fermentation of pomegranate bark residue by A. niger resulted in the production of citric acid (2) with reduced production costs, while adding value to a residue usually directed for disposal [ 137 ].…”

Section: Toward a Sustainable Production Of Fungal Metabolitesmentioning

confidence: 99%

Use of the Versatility of Fungal Metabolism to Meet Modern Demands for Healthy Aging, Functional Foods, and Sustainability

Takahashi

Barbosa

Martins

et al. 2020

JoF

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Aging-associated, non-transmissible chronic diseases (NTCD) such as cancer, dyslipidemia, and neurodegenerative disorders have been challenged through several strategies including the consumption of healthy foods and the development of new drugs for existing diseases. Consumer health consciousness is guiding market trends toward the development of additives and nutraceutical products of natural origin. Fungi produce several metabolites with bioactivity against NTCD as well as pigments, dyes, antioxidants, polysaccharides, and enzymes that can be explored as substitutes for synthetic food additives. Research in this area has increased the yields of metabolites for industrial applications through improving fermentation conditions, application of metabolic engineering techniques, and fungal genetic manipulation. Several modern hyphenated techniques have impressively increased the rate of research in this area, enabling the analysis of a large number of species and fermentative conditions. This review thus focuses on summarizing the nutritional, pharmacological, and economic importance of fungi and their metabolites resulting from applications in the aforementioned areas, examples of modern techniques for optimizing the production of fungi and their metabolites, and methodologies for the identification and analysis of these compounds.

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Bioengineering Fungi and Yeast for the Production of Enzymes, Metabolites, and Value-Added Compounds

Cited by 4 publications

References 143 publications

Focus and Insights into the Synthetic Biology-Mediated Chassis of Economically Important Fungi for the Production of High-Value Metabolites

Focus and Insights into the Synthetic Biology-Mediated Chassis of Economically Important Fungi for the Production of High-Value Metabolites

Multi-omics network model reveals key genes associated with p-coumaric acid stress response in an industrial yeast strain

Use of the Versatility of Fungal Metabolism to Meet Modern Demands for Healthy Aging, Functional Foods, and Sustainability

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