Bioengineering for Organ Transplantation: Progress and Challenges

Welman, Ted; Michel, Sebastian; Segaren, Nicholas; Shanmugarajah, Kumaran

doi:10.1080/21655979.2015.1081320

Cited by 30 publications

(19 citation statements)

References 50 publications

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“…In this regard, the 3D structure is essential for later cell survival and performance [Chan and Leong, 2008;Weber et al, 2008;Welman et al, 2015]. Implanting bioengineered organs and tissues such as artificial bladders [Atala et al, 2006], tracheas [Macchiarini et al, 2008], nasal cartilage [Fulco et al, 2014], and tubular tissue [Raya-Rivera et al, 2011 has been clinically studied.…”

Section: Discussionmentioning

confidence: 99%

Decellularized Pancreas Matrix Scaffolds for Tissue Engineering Using Ductal or Arterial Catheterization

Hashemi

Pasalar

Soleimani

et al. 2018

Cells Tissues Organs

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Introduction: Diabetes is known as a worldwide disease with a great burden on society. Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered. Bioscaffolds along with islet cells would provide bioengineered tissue as a substitute for β-cells. The perfusion-decellularization technique is considered to create such scaffolds since they mimic the compositional, architectural, and biomechanical nature of a native organ. In this study, we investigated 2 decellularization methods preserving tissue microarchitecture. Methods: Procured pancreas from Sprague-Dawley rats was exposed to different percentages of detergent for 2, 4, and 6 h after cannulation via the common bile duct or aorta. Results: High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure. In both methods, 6-h exposure to 0.05% SDS created a bioscaffold with intact extracellular matrices and proper biomechanical characteristics. Tissue-specific stainings revealed that elastic, reticular, and collagen fiber concentrations were well preserved. Quantitative findings showed that glycosaminoglycan content was slightly different, but hydroxyproline was in the range of native pancreas tissue. Dye infusion through ductal and vascular cannulation proved that the vascular network was intact, and scanning electron microscopy indicated a homogeneous porous structure. Conclusions: Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.

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Section: Discussionmentioning

confidence: 99%

Decellularized Pancreas Matrix Scaffolds for Tissue Engineering Using Ductal or Arterial Catheterization

Hashemi

Pasalar

Soleimani

et al. 2018

Cells Tissues Organs

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show abstract

“…Alginate, a scaffolding polysaccharide produced by brown seaweeds, has been widely employed by virtue of its biocompatibility [102,104,105]. Alginates are linear unbranched polymers containing β-(1 → 4)-linked Dmannuronic acid (M) and α-(1 → 4)-linked L-guluronic acid (G) residues and possess eminent gel-forming properties in the presence of polyvalent cations, such as Ca 2+ and Ba 2+ [103,[106][107][108]. Earlier studies have confirmed that compared to nonencapsulated islets, encapsulated islets have significantly improved survival, long-term biocompatibility and function with the use of purified alginate [109][110][111][112].…”

Section: Encapsulation Technique For Stem Cell Therapy For T1dmmentioning

confidence: 99%

Current progress in stem cell therapy for type 1 diabetes mellitus

2020

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Type 1 diabetes mellitus (T1DM) is the most common chronic autoimmune disease in young patients and is characterized by the loss of pancreatic β cells; as a result, the body becomes insulin deficient and hyperglycemic. Administration or injection of exogenous insulin cannot mimic the endogenous insulin secreted by a healthy pancreas. Pancreas and islet transplantation have emerged as promising treatments for reconstructing the normal regulation of blood glucose in T1DM patients. However, a critical shortage of pancreases and islets derived from human organ donors, complications associated with transplantations, high cost, and limited procedural availability remain bottlenecks in the widespread application of these strategies. Attempts have been directed to accommodate the increasing population of patients with T1DM. Stem cell therapy holds great potential for curing patients with T1DM. With the advent of research on stem cell therapy for various diseases, breakthroughs in stem cell-based therapy for T1DM have been reported. However, many unsolved issues need to be addressed before stem cell therapy will be clinically feasible for diabetic patients. In this review, we discuss the current research advances in strategies to obtain insulin-producing cells (IPCs) from different precursor cells and in stem cell-based therapies for diabetes.

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“…However, the ability of this bio-artificial heart to function in vivo, even in an heterotopic setting, was not tested. Moving to large-scale organs, Welman and colleagues 7 have reported that decellularized porcine scaffolds, recellularized by intracoronary infusion of human umbilical cord-derived endothelial cells and intramural injections of murine neonatal cardiac cells, could display myocardial electrical activity after 10 days of bioreactor perfusion, but here again, the functional correlate was not assessed, even ex vivo. Two recent studies have finally looked at human hearts.…”

Section: Act II I Have a Dream (From The Swedish Pop Group Abba)mentioning

confidence: 98%

Building a bioartificial heart: A 3-song saga

Menasché

2017

The Journal of Thoracic and Cardiovascular Surgery

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Bioengineering for Organ Transplantation: Progress and Challenges

Cited by 30 publications

References 50 publications

Decellularized Pancreas Matrix Scaffolds for Tissue Engineering Using Ductal or Arterial Catheterization

Decellularized Pancreas Matrix Scaffolds for Tissue Engineering Using Ductal or Arterial Catheterization

Current progress in stem cell therapy for type 1 diabetes mellitus

Building a bioartificial heart: A 3-song saga

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