Bioengineering, biomaterials, and β-cell replacement therapy

Vries, Rick de; Stell, Adam; Mohammed, Sami G.; Hermanns, Carolin; Martinez, Adela Helvia; Jetten, Marlon J.; Apeldoorn, A. van

doi:10.1016/b978-0-12-814831-0.00033-6

Cited by 10 publications

(8 citation statements)

References 241 publications

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“…Lastly, the agarose mold served as a neutral resting surface for the cultured ear, helping to retain its shape while permitting the diffusion of nutrients and growth factors from the culture media. Simultaneously, the poor cell attachment and migration into the agarose constructs reported in the literature [ 31 ] as advantageous in our setup as they inhibited cell migration from the cast construct into the agarose mold over time. It is worth noting that the ear could also be easily separated from the mold and transferred to a grid without any complications (Figure S7, Supporting Information).…”

Section: Discussionmentioning

confidence: 98%

Biofabrication of Heterogeneous, Multi‐Layered, and Human‐Scale Tissue Transplants Using Eluting Mold Casting

Tosoratti,

Rütsche,

Asadikorayem

et al. 2023

Adv Funct Materials

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The creation of multi‐tissue auricular transplants for the treatment of microtia is a challenge due to the complex and layered structure of this anatomical tissue. A novel casting technique for the 3D biofabrication of heterogeneous, multi‐layered, and human‐scale tissue transplants using eluting agarose molds is presented. The molds are generated by casting agarose into custom 3D‐printed containers, termed metamolds, optimized to facilitate the hydrogel casting process based on geometric and topological constraints. Casting yields high resolution (50 µm) and allows for subsequent casting of further hydrogel layers on the transplant. Multi‐layered auricular constructs are fabricated on a cartilage core consisting of a hyaluronic acid‐alginate double network and an adjacent gelatin‐based dermal layer. Bonding between adjacent layers is achieved by orthogonal physical and enzymatic crosslinking of residual functional groups between each layer. Material composition and culture duration are optimized for each layer allowing for maturation into cartilaginous and pre‐vascularized dermal tissues. To demonstrate the scalability of this technique for the biofabrication of human‐sized transplants, bi‐layered human‐sized ears are cast. Overall, this novel casting technique offers a promising approach for the fabrication of complex tissue grafts, overcoming the limitations of other traditional biofabrication methods.

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Section: Discussionmentioning

confidence: 98%

Biofabrication of Heterogeneous, Multi‐Layered, and Human‐Scale Tissue Transplants Using Eluting Mold Casting

Tosoratti,

Rütsche,

Asadikorayem

et al. 2023

Adv Funct Materials

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“…3). Prior reviews have summarized some of the main advances 89,90 and topics of interest 91 in relationship to bioprinting for T1D therapy, yet use of this biofabrication method to generate endocrine tissues is very limited, with only a few published works solely utilizing extrusion-based methods for incorporating pancreatic islets and/or beta cells into bioprinted constructs (Table 1).…”

Section: Current Landscapementioning

confidence: 99%

3D Bioprinting and Translation of Beta Cell Replacement Therapies for Type 1 Diabetes

Gurlin

Giraldo

Latres

2021

Tissue Engineering Part B: Reviews

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Type 1 diabetes (T1D) is an autoimmune disorder in which the body's own immune system selectively attacks beta cells within pancreatic islets resulting in insufficient insulin production and loss of the ability to regulate blood glucose (BG) levels. Currently, the standard of care consists of BG level monitoring and insulin administration, which are essential to avoid the consequences of dysglycemia and long-term complications. Although recent advances in continuous glucose monitoring and automated insulin delivery systems have resulted in improved clinical outcomes for users, nearly 80% of people with T1D fail to achieve their target hemoglobin A1c (HbA1c) levels defined by the American Diabetes Association. Intraportal islet transplantation into immunosuppressed individuals with T1D suffering from impaired awareness of hypoglycemia has resulted in lower HbA1c, elimination of severe hypoglycemic events, and insulin independence, demonstrating the unique potential of beta cell replacement therapy (BCRT) in providing optimal glycemic control and a functional cure for T1D. BCRTs need to maximize cell engraftment, long-term survival, and function in the absence of immunosuppression to provide meaningful clinical outcomes to all people living with T1D. One innovative technology that could enable widespread translation of this approach into the clinic is three-dimensional (3D) bioprinting. Herein, we review how bioprinting could facilitate translation of BCRTs as well as the current and forthcoming techniques used for bioprinting of a BCRT product. We discuss the strengths and weaknesses of 3D bioprinting in this context in addition to the road ahead for the development of BCRTs.

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“…95 This thermo reversible polymer can induce gelation or liquify at different temperatures varying from 20 C to 70 C, depending on the molecular weight and the degree of hydroxyethylation. 96 Due to the temperature-sensitive water solubility of this polymer, it is suitable for the cell encapsulation. Agarose does not provide active anchor for cells that limit its use in the adherent cell culture.…”

Section: Agarosementioning

confidence: 99%