Bioengineered vascular constructs as living models for in vitro cardiovascular research

Wolf, Frederic; Vogt, Felix; Schmitz‐Rode, Thomas; Jockenhoevel, Stefan; Mela, Petra

doi:10.1016/j.drudis.2016.04.017

Cited by 23 publications

(17 citation statements)

References 56 publications

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“…In this way, a regenerative, biological implant, which will not be attacked immunologically by the host, is converted successively to truly endogenous tissue in the course of the physiological remodelling. Tubular structures of fibrin and collagen can be constructed in vitro as an alternative to decellularisation of preformed donor tissue [26,72].…”

Section: The Ideal Vascular Prosthesismentioning

confidence: 99%

Cardiovascular Applications of Magnesium Alloys

Schilling¹,

Bauer²,

LaLonde³

et al. 2017

Magnesium Alloys

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Therapy in cardiovascular medicine often relies on implantation of prosthetic materials or application of stents. The diseases of many cardiovascular structures require their complete and immediate repair by utilising prosthetic materials. The ideal cardiovascular prosthesis involves good functional properties, capability of regeneration and does not activate the host's immune system. Ideally, the graft can be applied for a temporary use and degrades after a predefined period according to controlled degradation kinetics. Only biological grafts would provide this spectrum of properties by today's level of knowledge. However, biological prostheses exhibit some relevant drawbacks as well, such as insufficient mechanical stability or restricted availability. Implants or supporting structures of magnesium alloys would bridge this gap and would either provide a substrate for innovative and temporary grafts or would-as supporting structures-transiently add some missing properties to regenerative biological prostheses. This chapter reviews the different fields of cardiovascular therapeutic applications of magnesium alloys. The required properties of magnesium alloys and their preparation, fabrication and testing will be discussed under the specific cardiovascular perspective.

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Section: The Ideal Vascular Prosthesismentioning

confidence: 99%

Cardiovascular Applications of Magnesium Alloys

Schilling¹,

Bauer²,

LaLonde³

et al. 2017

Magnesium Alloys

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“…This vessel can, however, be functionalized with human endothelial cells cultured at the inner wall of the vessel while human perivascular cells (e.g., vascular smooth muscle cells, pericytes, and astrocytes) cultured in the wall surrounding the vessel [ 89 , 90 ]. Following functionalization, this blood vessel can be subjected to biochemical cues (e.g., low-density lipoprotein, high-density lipoprotein, and human whole blood) and mechanical cues (e.g., flow-induced shear stress) to create functional human in vitro atherosclerotic models [ 91 , 92 ]. This biomimetic model would be useful for exploring potential therapeutic effects of hMSCs in atherosclerosis for the prevention of tissue ischemia.…”

Section: Challenges Associated With Future Translation Of Hmscs Tomentioning

confidence: 99%

Mesenchymal Stem Cell Therapy for Ischemic Tissues

Yong

Choi

Mohammadi

et al. 2018

Stem Cells International

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Ischemic diseases such as myocardial infarction, ischemic stroke, and critical limb ischemia are immense public health challenges. Current pharmacotherapy and surgical approaches are insufficient to completely heal ischemic diseases and are associated with a considerable risk of adverse effects. Alternatively, human mesenchymal stem cells (hMSCs) have been shown to exhibit immunomodulation, angiogenesis, and paracrine secretion of bioactive factors that can attenuate inflammation and promote tissue regeneration, making them a promising cell source for ischemic disease therapy. This review summarizes the pathogenesis of ischemic diseases, discusses the potential therapeutic effects and mechanisms of hMSCs for these diseases, and provides an overview of challenges of using hMSCs clinically for treating ischemic diseases.

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“…In order to deal with the above-mentioned inter-species and inter-patient variability in the processes of material-driven inflammation and regeneration, the development of dedicated models is paramount. In vitro engineered laboratory models, based on human cells (either healthy or diseased) can be exploited to gain an initial understanding of tissue integration and remodeling in response to scaffolds (e.g., reviewed by 86 and 87 ). Dynamic in vitro co-culture platforms are eminently suitable to screen the interactions between human (circulatory) immune cells and valvular scaffolds under physiologically relevant hemodynamic stimuli, such as shear stress ( 88–90 ) and cyclic strains ( 91, 92 ).…”

Section: Outstanding Challengesmentioning

confidence: 99%

Can We Grow Valves Inside the Heart? Perspective on Material-based In Situ Heart Valve Tissue Engineering

Bouten

Smits

Baaijens

2018

Front. Cardiovasc. Med.

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In situ heart valve tissue engineering using cell-free synthetic, biodegradable scaffolds is under development as a clinically attractive approach to create living valves right inside the heart of a patient. In this approach, a valve-shaped porous scaffold “implant” is rapidly populated by endogenous cells that initiate neo-tissue formation in pace with scaffold degradation. While this may constitute a cost-effective procedure, compatible with regulatory and clinical standards worldwide, the new technology heavily relies on the development of advanced biomaterials, the processing thereof into (minimally invasive deliverable) scaffolds, and the interaction of such materials with endogenous cells and neo-tissue under hemodynamic conditions. Despite the first positive preclinical results and the initiation of a small-scale clinical trial by commercial parties, in situ tissue formation is not well understood. In addition, it remains to be determined whether the resulting neo-tissue can grow with the body and preserves functional homeostasis throughout life. More important yet, it is still unknown if and how in situ tissue formation can be controlled under conditions of genetic or acquired disease. Here, we discuss the recent advances of material-based in situ heart valve tissue engineering and highlight the most critical issues that remain before clinical application can be expected. We argue that a combination of basic science – unveiling the mechanisms of the human body to respond to the implanted biomaterial under (patho)physiological conditions – and technological advancements – relating to the development of next generation materials and the prediction of in situ tissue growth and adaptation – is essential to take the next step towards a realistic and rewarding translation of in situ heart valve tissue engineering.

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Bioengineered vascular constructs as living models for in vitro cardiovascular research

Cited by 23 publications

References 56 publications

Cardiovascular Applications of Magnesium Alloys

Cardiovascular Applications of Magnesium Alloys

Mesenchymal Stem Cell Therapy for Ischemic Tissues

Can We Grow Valves Inside the Heart? Perspective on Material-based In Situ Heart Valve Tissue Engineering

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