Bioengineered Graphene Oxide Microcomposites Containing Metabolically Versatile Paracoccus sp. MKU1 for Enhanced Catechol Degradation

Abstract

Paracoccus sp . MKU1, a metabolically versatile bacterium that encompasses diverse metabolic pathways in its genome for the degradation of aromatic compounds, was investigated for catechol bioremediation here for the first time to our knowledge. Paracoccus sp. MKU1 degraded catechol at an optimal pH of 7.5 and a temperature of 37 °C, wherein 100 mg/L catechol was completely mineralized in 96 h but required 192 h for complete mineralization of 500 mg/L catechol. While investigating the molecular mechanisms of its degradation potential, it was unveiled that Paracoccus sp . MKU1 employed both the ortho and meta pathways by inducing the expression of catechol 1,2-dioxygenase (C12O) and catechol 2,3-dioxygenase (C23O), respectively. C23O expression at transcriptional levels was significantly more abundant than C12O , which indicated that catechol degradation was primarily mediated by extradiol cleavage by MKU1. Furthermore, poly(MAA- co -BMA)-GO (PGO) microcomposites containing Paracoccus sp . MKU1 were synthesized, which degraded catechol (100 mg/L) completely within 48 h with excellent recycling performance for three cycles. Thus, PGO@ Paracoccus microcomposites proved to be efficient in catechol degradation at not only faster rates but also with excellent recycling performances than free cells. These findings accomplish that Paracoccus sp . MKU1 could serve as a potential tool for bioremediation of catechol-polluted industrial wastewater and soil.

Catechol 1,2-Dioxygenase From Paracoccus sp. MKU1—A Greener and Cleaner Bio-Machinery for cis, cis-Muconic Acid Production by Recombinant E. coli

“…Paracoccus sp. MKU1 is metabolically versatile bacteria that possess higher flexibility in utilizing wide range of recalcitrant xenobiotics ( Aravind et al, 2020 ). Genome analysis of Paracoccus sp.…”

“…Moreover, the expression of gene clusters corresponding to intradiol ( C12O ) and extradiol cleavage ( C23O ) at the transcriptional levels were found to be constitutive in Paracoccus sp. MKU1 and their expressions were significantly induced in the presence of catechol in a concentration dependent manner ( Aravind et al, 2020 ). The wild-type Paracoccus sp.…”

Analysis of Endophytic Bacterial Diversity From Different Dendrobium Stems and Discovery of an Endophyte Produced Dendrobine-Type Sesquiterpenoid Alkaloids

“…The strains BL-YJ10_15-29 ( P. pueri THG-N2.35, 98.98%) and GP-YJ15-74 ( Pseudomonas koreensis Ps 9-14, 98.80%) presented better antimicrobial activity. Although there have been many reports on genus Paracoccus , most of them have focused on its bioremediate aromatic compounds such as pyrene, halobenzoate, and pyridine; there are few reports about its antimicrobial activity ( Aravind et al, 2020 ; Nakkeeran et al, 2021 ). Therefore, more attention should be paid to the antibacterial activity of this genus in the future.…”

Biodegradation of Phenol by Bacillus simplex: Characterization and Kinetics Study

“…BZD-33 (Ke et al, 2019), Pseudomonas aeruginosa PDM (Al-Asoufi et al, 2017;Khleifat et al, 2019), Rhodococcus UKMP-5M (Suhaila et al, 2019). It has been observed that many of the oxygenase enzymes and the bacteria that harbor them are able to biodegrade different types of aromatic compounds as a substrate (Aravind et al, 2020;Khleifat et al, 2019;Mohite, 2015;Zou et al, 2018). It should be noted that many previous studies emphasized the necessity of providing an optimal amount of carbon and nitrogen sources such as yeast extract, to obtain the maximal rate of biodegradation of phenol or any other organic compounds.…”