Bioenergy technology for integrated production of polyhydroxybutyrate/bioplastic

Al-Battashi, Huda; Sivakumar, Nallusamy

doi:10.1016/b978-0-12-819597-0.00017-9

Cited by 2 publications

(1 citation statement)

References 121 publications

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“…Therefore, the simultaneous sacchari cation and fermentation (SSF) mode, integrating the enzymatic hydrolysis of the lignocellulosic waste and PHB production [16,17], was tried to overcome the drawback in SHF. Although the SHF was conducted at the optimal conditions for both sacchari cation and fermentation processes, applying SSF will reduce the production cost by simplifying the production process, avoiding end-product inhibition of the hydrolysis process, and reducing the enzyme loading [18][19][20][21]17]. In addition, the sugar concentration available for PHB accumulation can be increased by increasing the paper loading in SSF mode [22,23].…”

Section: Introductionmentioning

confidence: 99%

High-Solid Loading Enzymatic Hydrolysis of Waste Office Paper for poly-3-hydroxybutyrate Production Through Simultaneous Saccharification and Fermentation

Al-Battashi

Sivakumar

2022

J Polym Environ

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Waste paper holds great potential as a substrate for the microbial production of bioplastic ). This study aimed to produce PHB by utilizing o ce paper as a substrate using Cupriavidus necator through batch and fed-batch simultaneous sacchari cation and fermentation (SSF) approach. For the batch experiment, different loadings of shredded o ce paper (3, 5 and 10%) with two different pretreatments H 2 O 2 (OPH) and H 2 O 2 and Triton X-100 (OPTH) were carried out. For the fed-batch experiment, paper loading started with 3% and two more additions were made at 36 and 84 h. Both experiments were conducted at 30°C, 200 rpm and pH 7 using 55.5 FPU/g of cellulase and 37.5 CBU/g of β-glucosidase with a xed amount of nitrogen source. High PHB yield was observed with OPH in all loadings, though the OPHT showed a better hydrolysis. Maximum PHB yield (4.27 g/L) was achieved with 10% OP at six days of fermentation in batch SSF. Whereas, maximum PHB yield (4.19 g/L) was obtained within a shorter time (66 h) in the fed-batch OPH paper. The extracted PHB showed wellmatched characteristic features to the standard PHB. Finally, this study proves the feasibility of employing SSF process for PHB production using waste paper as an alternative approach to overcome the shortcoming of the separate hydrolysis and fermentation (SHF) process.

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Section: Introductionmentioning

confidence: 99%

High-Solid Loading Enzymatic Hydrolysis of Waste Office Paper for poly-3-hydroxybutyrate Production Through Simultaneous Saccharification and Fermentation

Al-Battashi

Sivakumar

2022

J Polym Environ

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High-Solid Loading Enzymatic Hydrolysis of Waste Office Paper for Poly-3-Hydroxybutyrate Production Through Simultaneous Saccharification and Fermentation

Al-Battashi

Sivakumar

2021

Preprint

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Waste paper holds great potential as a substrate for the microbial production of bioplastic (Poly-3-hydroxybutyrate (PHB)). This study aimed to produce PHB by utilizing office paper as a substrate using Cupriavidus necator through batch and fed-batch simultaneous saccharification and fermentation (SSF) approach. For the batch experiment, different loadings of shredded office paper (3, 5 and 10%) with two different pretreatments H2O2 (OPH) and H2O2 and Triton X-100 (OPTH) were carried out. For the fed-batch experiment, paper loading started with 3% and two more additions were made at 36 and 84 h. Both experiments were conducted at 30°C, 200 rpm and pH 7 using 55.5 FPU/g of cellulase and 37.5 CBU/g of β-glucosidase with a fixed amount of nitrogen source. High PHB yield was observed with OPH in all loadings, though the OPHT showed a better hydrolysis. Maximum PHB yield (4.27 g/L) was achieved with 10% OP at six days of fermentation in batch SSF. Whereas, maximum PHB yield (4.19 g/L) was obtained within a shorter time (66 h) in the fed-batch OPH paper. The extracted PHB showed well-matched characteristic features to the standard PHB. Finally, this study proves the feasibility of employing SSF process for PHB production using waste paper as an alternative approach to overcome the shortcoming of the separate hydrolysis and fermentation (SHF) process.

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Bioenergy technology for integrated production of polyhydroxybutyrate/bioplastic

Cited by 2 publications

References 121 publications

High-Solid Loading Enzymatic Hydrolysis of Waste Office Paper for poly-3-hydroxybutyrate Production Through Simultaneous Saccharification and Fermentation

High-Solid Loading Enzymatic Hydrolysis of Waste Office Paper for poly-3-hydroxybutyrate Production Through Simultaneous Saccharification and Fermentation

High-Solid Loading Enzymatic Hydrolysis of Waste Office Paper for Poly-3-Hydroxybutyrate Production Through Simultaneous Saccharification and Fermentation

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