Bioenergetic aspects of bacteriophage replication in the photosynthetic bacterium Rhodopseudomonas capsulata

Schmidt, Laura S.; Yen, Huei-Che; Gest, Howard

doi:10.1016/0003-9861(74)90159-3

Cited by 18 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some years ago we isolated, from sewage, a virulent phage active on R. capsulatus, and examined its replication under various conditions (Schmidt et al 1974). In photosynthetically grown cells, phage replication was supported by anaerobic photophosphorylation, and virus growth in illuminated infected cells was markedly suppressed by inhibitors of photophosphorylation.…”

Section: Bacteriophage Againmentioning

confidence: 99%

A microbiologist's odyssey: Bacterial viruses to photosynthetic bacteria

Gest

1994

Photosynth Res

Self Cite

View full text Add to dashboard Cite

Perspective can be defined as the relationships or relative importance of facts or matters from any special point of view. Thus, my Personal perspective reflects the threads I followed in a 50-year journey of research in the complex tapestry of bioenergetics and various aspects of microbial metabolism. An early interest in biochemical and microbial evolution led to the fertile hunting grounds of anoxygenic photosynthetic bacteria. Viewed as a physiological class, these organisms show remarkable metabolic versatility in that certain individual species are capable of using all the known major types of energy conversion (photosynthetic, respiratory, and fermentative) to support growth. Since such anoxyphototrophs are readily amenable to molecular genetic/biological manipulation, it can be expected that they will eventually provide important clues for unraveling the evolutionary relationships of the several kinds of energy conversion. I gradually came to believe that understanding the evolution of phototrophs would require detailed knowledge not only of how light is converted to chemical energy, but also of a) pathways of monomer production from extracellular sources of carbon and nitrogen and b) mechanisms cells use for integrating ATP regeneration with the energy-requiring biosyntheses of biological macromolecules. Serendipic observation of photoproduction of H2 from organic compounds by Rhodospirillum rubrum in 1949 led to discovery of N2 fixation by anoxyphototrophs, and this capacity was later exploited for the isolation of hitherto unknown species of photosynthetic prokaryotes, including the heliobacteria. Recent studies on the reaction centers of the heliobacteria suggest the possibility that these bacteria are descendents of early phototrophs that gave rise to oxygenic photosynthetic organisms.

show abstract

Section: Bacteriophage Againmentioning

confidence: 99%

A microbiologist's odyssey: Bacterial viruses to photosynthetic bacteria

Gest

1994

Photosynth Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…The first report of a virulent phage specific for a member of the Rhodospirillaceae described phage Rpl of R. palustris (66). Both virulent and temperate phages specific for R. sphaeroides (1,153) and R. capsulata (208,263) have subsequently been isolated and characterized. Their properties have recently been documented in detail (143).…”

Section: Bacteriophage and Bacteriocinsmentioning

confidence: 99%

“…In this regard, photosynthetic procaryotes undoubtedly offer distinct advantages over other procaryotes, primarily because the energy status of the host cell can be conveniently manipulated merely by adjusting such parameters as light intensity. Schmidt et al (208), investigating the bioenergetics of bacteriophage RC1 replication in R. capsulata, concluded that the energy requirement for the replication of this phage is more critical than that for uninfected host cell growth. In photosynthetically grown host cells, phage RC1 replication could be supported either by photophosphorylation or oxidative phosphorylation.…”

Section: Bacteriophage and Bacteriocinsmentioning

confidence: 99%

Genetics of Rhodospirillaceae.

Saunders¹

1978

Microbiological Reviews

View full text Add to dashboard Cite

“…These include bacteriophage RSI, a lytic phage isolated from sewage (Abeliovich & Kaplan, 1974), ϕRsG1 and Rϕ1, both temperate bacteriophage (Duchrow & Giffhorn, 1987; Mural & Friedman, 1974), and Rϕ6P (Tucker & Pemberton, 1978), which exists as a plasmid during lysogeny. A few bacteriophage have been isolated for Rhodobacter capsulatus and their host ranges determined (Schmidt et al ., 1974). RC1 is a virulent bacteriophage of Rhodobacter capsulatus which has been used in one of the very few studies of the bioenergetics of bacteriophage replication (Schmidt et al ., 1974).…”

Section: Introductionmentioning

confidence: 99%

A bacteriophage of Rhodopseudomonas blastica

Gorham

Dow

1996

Microbiology

View full text Add to dashboard Cite

A bacteriophage, 4BHG1, was isolated from a small eutrophic pond from which its host, Rhodopseudomonas blastica, was originally obtained. It is a lytic bacteriophage specific for R. blastica which also causes non-specific lysis of Rhodobacter sphaeroides 8253. 4BHGl has an icosahedral head of 62 nm diameter and a short 39 nm tail. Caesium chloride density gradient centrifugation of infected cell lysates gave a single bacteriophage band a t a density of 1.385 g ~m -~, but also occasionally a second band was observed at a lower density. No differences were apparent between bacteriophage taken from either of the two bands. 4BHG1 contained double-stranded DNA with a size of 48 kb and a G +C content of 50.6 mol%. The bacteriophage adsorbed to both photosynthetically and chemoheterotrophically grown R. blastica a t an identical rate of 1-39 x studies of the bacteriophage under these physiological regimes showed no differences in the latent and rise periods and only slight changes in the burst size. Adsorption of this bacteriophage is cell-surface specific and attachment only occurs to the 'older' pole of the budding reproductive cell.ml-1 mi+. One-step growth curves and kinetic

show abstract

Bioenergetic aspects of bacteriophage replication in the photosynthetic bacterium Rhodopseudomonas capsulata

Cited by 18 publications

References 19 publications

A microbiologist's odyssey: Bacterial viruses to photosynthetic bacteria

A microbiologist's odyssey: Bacterial viruses to photosynthetic bacteria

Genetics of Rhodospirillaceae.

A bacteriophage of Rhodopseudomonas blastica

Contact Info

Product

Resources

About