Bioelectromagnetic Field Effects on Cancer Cells and Mice Tumors

Berg, Hermann; Günther, Bernd; Hilger, Ingrid; Radeva, Maria; Traitcheva, Nelly; Wollweber, Leo

doi:10.3109/15368371003776725

Cited by 34 publications

(31 citation statements)

References 31 publications

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“…It should be noted that electromagnetic (EM) fields may affect the regulation of cellular growth and differentiation, including the growth of tumors [9,10]. Both static magnetic and electric fields have altered the mitotic index and cell cycle progression of a number of cell types in various species [10].…”

Section: Introductionmentioning

confidence: 99%

An Overview of Sub-Cellular Mechanisms Involved in the Action of TTFields

Tuszyński

Wenger

Friesen

et al. 2016

IJERPH

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Long-standing research on electric and electromagnetic field interactions with biological cells and their subcellular structures has mainly focused on the low- and high-frequency regimes. Biological effects at intermediate frequencies between 100 and 300 kHz have been recently discovered and applied to cancer cells as a therapeutic modality called Tumor Treating Fields (TTFields). TTFields are clinically applied to disrupt cell division, primarily for the treatment of glioblastoma multiforme (GBM). In this review, we provide an assessment of possible physical interactions between 100 kHz range alternating electric fields and biological cells in general and their nano-scale subcellular structures in particular. This is intended to mechanistically elucidate the observed strong disruptive effects in cancer cells. Computational models of isolated cells subject to TTFields predict that for intermediate frequencies the intracellular electric field strength significantly increases and that peak dielectrophoretic forces develop in dividing cells. These findings are in agreement with in vitro observations of TTFields’ disruptive effects on cellular function. We conclude that the most likely candidates to provide a quantitative explanation of these effects are ionic condensation waves around microtubules as well as dielectrophoretic effects on the dipole moments of microtubules. A less likely possibility is the involvement of actin filaments or ion channels.

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Section: Introductionmentioning

confidence: 99%

An Overview of Sub-Cellular Mechanisms Involved in the Action of TTFields

Tuszyński

Wenger

Friesen

et al. 2016

IJERPH

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“…While several studies suggested that EMF exposure induces immune cell activation and DNA damage (10,11), the findings of other studies indicated that the effects of EMF inhibit preneoplastic lesions, resulting in the reduction of cell proliferation (3). A number of studies focused on the potential effects of EMF at cancer treatment (12,13).…”

Section: Discussionmentioning

confidence: 99%

P-selectin expression in a colon tumor model exposed by sinusoidal electromagnetic fields

et al. 2013

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Abstract. P-selectin is mainly involved in the initial process of tumor cell adhesion to platelets. The aim of the present study was to determine the expression level of P-selectin in a colon tumor model affected by sinusoidal electromagnetic fields (SMF). Male Wistar albino rats aged 2-2.5 months were used. The animals were divided into the I [N-Methyl-N-Nitrosurea (MNU)], II (SMF-MNU), III (SMF) and IV (control) groups. The rats were housed five per polycarbonate cage. Sixty milligrams of MNU was dissolved in 6 ml sterile 0.9% NaCl. Prepared solutions were administered intra rectally (i.r.) to the 1st and 3rd groups as 0.2 ml/per animal. The same procedure was applied to the 2nd and 4th groups, although 0.2 ml/per animal sterile isotonic solution was administered instead. This procedure was repeated once a week for 10 weeks. Following the administration of MNU, the 2nd and 3rd groups were exposed to a sinusoidal magnetic field (SMF, 50 Hz, 5 mT) for 6 h/day for 8 months. P-selectin expression of the four groups of rat colon tissues was determined using immunohistochemistry on paraffin sections. The labeled streptavidin biotin method was performed. Fisher's exact test was used for differences between proportions. Results showed that there was no statistically significant (P>0.05) change in the expression level of P-selectin. However, this result should be verified by both in vivo and in vitro experiments to determine the effects of the magnetic fields on P-selectin.

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“…Berg also illustrated effects of bioelectromagnetic in the mT amplitude range on cancer cells on mice. He fide out that synergic combination of extremely low-frequency (ELF) pulsating electromagnetic fields (PEMF) and sinusoidal electromagnetic fields (SEMF) induce tumor cell apoptosis, inhibit angiogenesis and proliferation of cells and cause necrosis [19]. Another research reveals that the expression levels of angiopoietin-2 and fibroblast growth factor-2 were significantly higher in the PEMF group than in the control group while lack of rise in the expression level of vascular endothelial growth factor suggests that PEMF does not have invasive effects including the induction of hypoxic conditions and inflammation on the bone marrow [20].…”

Section: Discussionmentioning

confidence: 99%

Reduction VEGF-A expression induced by saffron aqua extract with low frequency electromagnetic field in MCF-7 cell line

Mousavi¹,

Baharara²

2014

The Journal of Genes, Microbes and Immunity

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Background: Studies both invivo and invitro indicated anticancer and antitumor properties of saffron and its component against different human cancer cells which is wildly used as a drug to promote health and fight diseases from ancient time. Angiogenesis, formation of new blood vessels, is regulated by the balances of many stimulating and inhibiting factors. While physiological angiogenesis is under strike control, disruption of this control causes proliferation of a network of blood vessels penetrating into cancerous growth. One of the main factors which are involved in the process of angiogenesis in both physiologic and pathologic condition is VEGF-A. On the other hand, bio electromagnetic fields have been shown to be clinically beneficial in the mT amplitude range. The aim of this study was finding out if synergic effects of saffron aqueous extract and low-frequency electromagnetic field could decrease angiogenesis via reduction of VEGF-A gene expression.Materials and methods: In this experimental study, saffron extract obtained using lyophilized by freeze dryer. MCF-7 cells were grown in RPMI 1640 medium supplemented with %10 FBS and incubated at 37˚C with %5 CO2. After 24h cells were treated by saffron aqueous extract at concentration of 100, 200, 400 and 800 µg/ml. 48 h after treatment all flasks exposed with electromagnetic field then total RNA extracted, cDNA synthesized using the sequence of target gene. Finally synthesized products analyzed by Real Time PCR to determine expression level of VEGF-A. The quantitative data analyzed by SPSS software. P value ˂0.05 was considered statistically significant.Results: Data analysis indicate inhibitory effect of saffron extract in concentration 100, 200, 400 and 800µg/ml on VEGF-A gene expression in MCF-7 cell line in compare with control. Most reduction in gene expression can be seen in the highest concentration of saffron extract (800 µg/ml) with 17% reduction on gene expression (P˂0.001), while there is no difference between 100 and 200 µg/ml (P˃0.05) but comparing to control group it is also fall noticeable with 11% and 12% respectively (P˂0.05). Synergic use of low frequency electromagnetic field (50Hz, 0.004 T) and saffron aqua extract shows reduction in concentration 100 to 800 µg/ml with 17%, 13%, 15% and 19% respectively and was significant at concentration of 800µg/ml (P˂0.001).Conclusion: Results shows decrease in the expression of VEGF-A in the treated samples with saffron extract and low frequency electromagnetic field which may introduce promising treatment against malignant and tumor metastasis, although more researches needs to figure out its accurate mechanism.

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Bioelectromagnetic Field Effects on Cancer Cells and Mice Tumors

Cited by 34 publications

References 31 publications

An Overview of Sub-Cellular Mechanisms Involved in the Action of TTFields

An Overview of Sub-Cellular Mechanisms Involved in the Action of TTFields

P-selectin expression in a colon tumor model exposed by sinusoidal electromagnetic fields

Reduction VEGF-A expression induced by saffron aqua extract with low frequency electromagnetic field in MCF-7 cell line

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