Bioelectrocatalysis for Biofuel Cells

Casanova-Moreno, Jannu; Arjona, Noé; Cercado, Bibiana

doi:10.1002/9781119725008.ch1

Cited by 3 publications

(1 citation statement)

References 169 publications

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“…In cross-linking, the reactants form covalent bonds with enzyme surface groups. Most of the known cross-linkers, such as ethylene glycol diglycidyl ether (EGDGE) and glutaraldehyde (GA), have epoxy or aldehyde groups in their ends [ 25 ]. These groups can react with amine, carboxyl, and hydroxyl groups.…”

Section: Introductionmentioning

confidence: 99%

Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems

Navarro-Nateras,

Diaz-Gonzalez,

Aguas-Chantes

et al. 2023

Biosensors

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The inclusion of online, in situ biosensors in microfluidic cell cultures is important to monitor and characterize a physiologically mimicking environment. This work presents the performance of second-generation electrochemical enzymatic biosensors to detect glucose in cell culture media. Glutaraldehyde and ethylene glycol diglycidyl ether (EGDGE) were tested as cross-linkers to immobilize glucose oxidase and an osmium-modified redox polymer on the surface of carbon electrodes. Tests employing screen printed electrodes showed adequate performance in a Roswell Park Memorial Institute (RPMI-1640) media spiked with fetal bovine serum (FBS). Comparable first-generation sensors were shown to be heavily affected by complex biological media. This difference is explained in terms of the respective charge transfer mechanisms. Under the tested conditions, electron hopping between Os redox centers was less vulnerable than H2O2 diffusion to biofouling by the substances present in the cell culture matrix. By employing pencil leads as electrodes, the incorporation of these electrodes in a polydimethylsiloxane (PDMS) microfluidic channel was achieved simply and at a low cost. Under flow conditions, electrodes fabricated using EGDGE presented the best performance with a limit of detection of 0.5 mM, a linear range up to 10 mM, and a sensitivity of 4.69 μA mM−1 cm−2.

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Section: Introductionmentioning

confidence: 99%

Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems

Navarro-Nateras,

Diaz-Gonzalez,

Aguas-Chantes

et al. 2023

Biosensors

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Ultrasonic pre-treatment of Bacillus velezensis for improved electrogenic response in a single chambered microbial fuel cell

et al. 2021

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Various microbial strains and techniques are being used to improve power production in microbial fuel cells. Cow dung is a peculiar source of anaerobic and micro-aerophilic organisms that were employed in this study to isolate exo-electrogenic microorganisms. To validate their exo-electrogenic nature, all eight visually distinct bacterial single-cell colonies were tested using the ferrocyanide reduction assay, which resulted in the selection of one bacterial strain AD1-ELB with the ability to reduce ferrocyanide for further biochemical, physiological and electrochemical characterization. The selected strain AD1-ELB was identified as Bacillus velezensis by 16 s rRNA gene sequencing. When used in a single-chambered MFC, the isolated AD1-ELB strain produced a maximum open-circuit voltage of 455 mV with a maximum current density of 51.78 µA/cm 2 and maximum power density of 4.33 µW/cm 2 on the 16th day. Bacillus velezensis AD1-ELB strain was treated with lowfrequency ultrasound (40 kHz) for 1, 2, 3, 4, and 5 min to assess the effect of ultrasonic pre-treatment on an isolated pure culture-based microbial fuel cell. A 3-min exposure to low-frequency ultrasonic therapy resulted in an increase in maximum power of 4.33 µW/cm 2 with a current density of 51.78 µA/cm 2 in the MFC, which decreases significantly after 4 and 5 min. Thus, the overall power density achieved was 1.89 times greater than in MFCs with untreated strain. These findings support the use of low-frequency ultrasonic stimulation to improve the performance of microbial fuel cell devices and are restricted to the pure, single-cell strain AD1-ELB, with the potential for variation if some other isolated strain is utilized, hence requiring further study to determine its relative variations.

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The novel advancements of nanomaterials in biofuel cells with a focus on electrodes’ applications

Tawalbeh

Javed

Al‐Othman

et al. 2022

Fuel

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Bioelectrocatalysis for Biofuel Cells

Cited by 3 publications

References 169 publications

Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems

Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems

Ultrasonic pre-treatment of Bacillus velezensis for improved electrogenic response in a single chambered microbial fuel cell

The novel advancements of nanomaterials in biofuel cells with a focus on electrodes’ applications

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