Bioelectrocatalysis-based dihydrogen/dioxygen fuel cell operating at physiological pH

Tsujimura, Seiya; Fujita, Masafumi; Tatsumi, Hirosuke; Kano, Kenji; Ikeda, Tokuji

doi:10.1039/b009539g

Cited by 152 publications

(101 citation statements)

References 26 publications

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“…These performance metrics were reproducibly observed throughout the 2-year operation of the MFC system 18 . The potentiostatic operation (S1), with the anodic potential controlled to þ 100 mV versus SHE allowed for the measurement of anodic microbial activity independent of system limitations 18,19 , resulted in a current density that was 8 times higher (550 mA m À 2 ) than that observed for the MFC condition, and the electron recovery efficiency was B50%. During the OC condition (S2) the anode electrode was disconnected from the cathode electrode and operated with zero current production and 0% electron recovery.…”

Section: Resultsmentioning

confidence: 99%

A novel metatranscriptomic approach to identify gene expression dynamics during extracellular electron transfer

et al. 2013

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Microbial respiration via extracellular electron transfer (EET) is a ubiquitous reaction that occurs throughout anoxic environments and is a driving force behind global biogeochemical cycling of metals. Here we identify specific EET-active microbes and genes in a diverse biofilm using an innovative approach to analyse the dynamic community-wide response to changing EET rates. We find that the most significant gene expression responses to applied EET stimuli occur in only two microbial groups, Desulfobulbaceae and Desulfuromonadales. Metagenomic analyses reveal high coverage draft genomes of these abundant and active microbes. Our metatranscriptomic results show known and unknown genes that are highly responsive to EET stimuli and associated with our identified draft genomes. This new approach yields a comprehensive image of functional microbes and genes related to EET activity in a diverse community, representing the next step towards unravelling complex microbial roles within a community and how microbes adapt to specific environmental stimuli.

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Section: Resultsmentioning

confidence: 99%

A novel metatranscriptomic approach to identify gene expression dynamics during extracellular electron transfer

et al. 2013

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“…Systems biology for electrogenic biofilms S Ishii et al system limitations, anode and cathode polarization curves were determined by linear sweep voltammetry (Tsujimura et al, 2001). The curves clearly showed that the cathode polarization strongly affected the whole MFC system polarization; however, the anode polarization curves showed that anodic limiting current densities significantly increased from around 0.1 mA cm À 2 to 0.3 mA cm À 2 during the 3-month operation in all MFCs (Figures 3a-d).…”

Section: Limiting Current Densitymentioning

confidence: 99%

“…In order to obtain anode polarization curves, an Ag/AgCl reference electrode (RE-5B, BASi) was placed in the side port of the reactors, and linear sweep voltammetry analyses were conducted using a potentiostat (Reference 600 or MultEchem System, Gamry) (Tsujimura et al, 2001;Ishii et al, 2008). The anode potential was swept from open-circuit anode potential to þ 300 mV vs SHE at a scan rate of 0.5 mV s À 1 and the corresponding anodic current was recorded.…”

Section: Polarization Analysesmentioning

confidence: 99%

Microbial population and functional dynamics associated with surface potential and carbon metabolism

Ishii¹,

et al. 2013

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Microbial extracellular electron transfer (EET) to solid surfaces is an important reaction for metal reduction occurring in various anoxic environments. However, it is challenging to accurately characterize EET-active microbial communities and each member's contribution to EET reactions because of changes in composition and concentrations of electron donors and solid-phase acceptors. Here, we used bioelectrochemical systems to systematically evaluate the synergistic effects of carbon source and surface redox potential on EET-active microbial community development, metabolic networks and overall electron transfer rates. The results indicate that faster biocatalytic rates were observed under electropositive electrode surface potential conditions, and under fatty acid-fed conditions. Temporal 16S rRNA-based microbial community analyses showed that Geobacter phylotypes were highly diverse and apparently dependent on surface potentials. The well-known electrogenic microbes affiliated with the Geobacter metallireducens clade were associated with lower surface potentials and less current generation, whereas Geobacter subsurface clades 1 and 2 were associated with higher surface potentials and greater current generation. An association was also observed between specific fermentative phylotypes and Geobacter phylotypes at specific surface potentials. When sugars were present, Tolumonas and Aeromonas phylotypes were preferentially associated with lower surface potentials, whereas Lactococcus phylotypes were found to be closely associated with Geobacter subsurface clades 1 and 2 phylotypes under higher surface potential conditions. Collectively, these results suggest that surface potentials provide a strong selective pressure, at the species and strain level, for both solid surface respirators and fermentative microbes throughout the EET-active community development.

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“…[290] The first biofuel cell based on H 2 oxidation and O 2 reduction was reported late in 2001. [291] The enzyme for O 2 reduction was a BOD from Mv. H 2 oxidation was not realized by enzymes themselves but by whole cells of the sulfate reducing bacterium Desulfovibrio vulgaris Hildenborough (DvH), which is known to contain several Hases from different classes.…”

Section: H 2 /O 2 Biofuel Cells: a Short But Attractive Storymentioning

confidence: 99%

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

et al. 2014

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Among sustainable alternatives to fossil fuel, proton exchange membrane fuel cells are promising devices that deliver electricity from hydrogen and oxygen, producing only water. The transformation of the fuel and oxidant however relies on rare‐metal catalysts. H2/O2 enzymatic biofuel cells thus emerge as sustainable biotechnological devices in which chemical catalysts are replaced by hydrogenases at the anode and multicopper oxidases at the cathode. This review discusses the recent breakthroughs and the limitations in all the components of H2/O2 biofuel cells: 1) Catalytic mechanisms involved in multicopper oxidases and hydrogenases, in addition to the new potential of enzymes from the biodiversity pool, which exhibit outstanding properties. 2) The molecular basis for oriented enzyme immobilization on the electrochemical interfaces as a prerequisite for a fast direct electron‐transfer rate. 3) The requirement for 3D networks to enhance current densities. 4) The production of H2 from biomass. 5) The history of H2/O2 biofuel cells and recent devices, which also highlights the remaining issues that will allow the use of such devices in low‐power applications.

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Bioelectrocatalysis-based dihydrogen/dioxygen fuel cell operating at physiological pH

Cited by 152 publications

References 26 publications

A novel metatranscriptomic approach to identify gene expression dynamics during extracellular electron transfer

A novel metatranscriptomic approach to identify gene expression dynamics during extracellular electron transfer

Microbial population and functional dynamics associated with surface potential and carbon metabolism

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

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Bioelectrocatalysis-based dihydrogen/dioxygen fuel cell operating at physiological pH

Cited by 152 publications

References 26 publications

A novel metatranscriptomic approach to identify gene expression dynamics during extracellular electron transfer

A novel metatranscriptomic approach to identify gene expression dynamics during extracellular electron transfer

Microbial population and functional dynamics associated with surface potential and carbon metabolism

Biohydrogen for a New Generation of H2/O2 Biofuel Cells: A Sustainable Energy Perspective

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Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective