Bioelectric mechanisms in regeneration: Unique aspects and future perspectives

Levin, Michael

doi:10.1016/j.semcdb.2009.04.013

Cited by 177 publications

(171 citation statements)

References 220 publications

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“…Wound healing, ion flux, and interactions between the wound epidermis and the underlying tissue appear urgently important for regenerative outgrowth. (16,18) Recent work offers that programmed cell death may play a role in triggering regenerative responses in many different organisms. (19) Apoptotic cells have been observed during early phases of regeneration in several animals that can regenerate missing tissues, for examples planarians, xenopus, and newts.…”

Section: Introductionmentioning

confidence: 99%

Stem Cell Therapy in Wound Healing and Tissue Regeneration

2016

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BACKGROUND: Recent advances in our basic knowledge of the tissue damage and regeneration pathology have combined with a remarkable progress in stem cell biology so the prospect of clinical tissue repair strategies is a tangible reality. We tried to describe a better view about mesenchymal stem cell (MSC) mechanisms in wound healing and tissue regeneration, sending any ideas for next advanced therapies.CONTENT: Sustaining injury, whether minor or major, is part of every organism life. Therefore, efficient response mechanisms to damage have developed. Wound healing is a perplexing multi-step processes which can be divided into three major phases: inflammation, proliferation, and scar formation/remodeling. Though the compartementalization of this process into discrete stages give the illusion of simplicity, but in reality it is much more complicated. So that efficient healing can occur, complex interactions between multiple cell types, soluble factors and extracellular matrix components are required to rebuild the tissue. Even under optimal conditions, the healing process drives to fibrosis or scar. The latest technology that makes a huge difference in the wound healing process is stem cell therapy, which offers a novel approach to many diseases.SUMMARY: Wound healing therapies continue to rapidly evolve, with advances in basic science and engineering research heralding the development of new therapies, as well as ways to modify existing treatments. Stem cell-based therapy is one of the most promising therapeutic concepts for wound healing. Advances in stem cell biology have enabled researchers and clinicians alike with access to cells capable of actively modulating the healing response. KEYWORDS: wound healing, tissue regeneration, stem cells therapy

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Section: Introductionmentioning

confidence: 99%

Stem Cell Therapy in Wound Healing and Tissue Regeneration

2016

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“…55 And in a reciprocal relationship, the induced voltage can regulate the ion flow. 55,56 An electric field can be formed in a polarized cell membrane, which can influence the voltage and ion flux of the adjacent cells so that cells can act in correlation. [55][56][57] The conductivity of the scaffold facilitates such electrical communication among cells.…”

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confidence: 99%

3D conductive nanocomposite scaffold for bone tissue engineering

Shahini¹,

Yazdimamaghani²,

Walker³

et al. 2013

IJN

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Bone healing can be significantly expedited by applying electrical stimuli in the injured region. Therefore, a three-dimensional (3D) ceramic conductive tissue engineering scaffold for large bone defects that can locally deliver the electrical stimuli is highly desired. In the present study, 3D conductive scaffolds were prepared by employing a biocompatible conductive polymer, ie, poly(3,4-ethylenedioxythiophene) poly(4-styrene sulfonate) (PEDOT:PSS), in the optimized nanocomposite of gelatin and bioactive glass. For in vitro analysis, adult human mesenchymal stem cells were seeded in the scaffolds. Material characterizations using hydrogen-1 nuclear magnetic resonance, in vitro degradation, as well as thermal and mechanical analysis showed that incorporation of PEDOT:PSS increased the physiochemical stability of the composite, resulting in improved mechanical properties and biodegradation resistance. The outcomes indicate that PEDOT:PSS and polypeptide chains have close interaction, most likely by forming salt bridges between arginine side chains and sulfonate groups. The morphology of the scaffolds and cultured human mesenchymal stem cells were observed and analyzed via scanning electron microscope, micro-computed tomography, and confocal fluorescent microscope. Increasing the concentration of the conductive polymer in the scaffold enhanced the cell viability, indicating the improved microstructure of the scaffolds or boosted electrical signaling among cells. These results show that these conductive scaffolds are not only structurally more favorable for bone tissue engineering, but also can be a step forward in combining the tissue engineering techniques with the method of enhancing the bone healing by electrical stimuli.

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“…These electric field gradients ("bioelectricity") are not only created by small ions but are also driven by larger biomolecules. These charge driven electric fields trigger pathways such as cell signaling, tissue factors, growth hormones, transmitters etc [1][2][3][4][5][6][7]. Since about ten years from now, new methods like membrane potential-and ion-sensitive in vivo dyes as well as constructs for imaging and molecular tracing are available, allowing a direct observation of the mentioned processes in cells, tissues and living systems.…”

Section: Equivalent Counterpart In the Organism?mentioning

confidence: 99%

Does electromagnetic therapy meet an equivalent counterpart within the organism?

Funk¹

2017

J Transl Sci

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This review bundles all available new information about intrinsic electrical phenomena in many types of cells (also non-neural) and tissues and shows that exogenously applied electric fields (as DC -EF, EMF or pulsed electromagnetic fields PEMF) can couple to the endogenous electrical phenomena of the body. These endogenous fields are generated ubiquitously in all tissues via cellular ion pumps and transporters and these ion gradients can be transferred by gap junctions. Such electric phenomena can now be monitored in living cells and tissues by electro-sensitive markers. Observations are now available from early embryonic development on to wound healing and regeneration within the adult organism. Based on these grounds we demonstrate that endogenous electric fields act directly on classical pathways of molecular and cell biology. Adequately applied frequencies and pulsing from outside can couple to these endogenous fields or to the receptors of classical biochemical pathways and manifest in positive "reprogramming" of tissue functions. In sum, this new analytical approach to the bodies´ own electrical information processes opens up new avenues for adequate EMF and PEMF therapy.

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Bioelectric mechanisms in regeneration: Unique aspects and future perspectives

Cited by 177 publications

References 220 publications

Stem Cell Therapy in Wound Healing and Tissue Regeneration

Stem Cell Therapy in Wound Healing and Tissue Regeneration

3D conductive nanocomposite scaffold for bone tissue engineering

Does electromagnetic therapy meet an equivalent counterpart within the organism?

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