Bioefficacy of Graviola leaf extracts in scavenging free radicals and upregulating antioxidant genes

Son, Yura; Choi, Eun‐Hye; Kim, Goon‐Tae; Park, Tae‐Sik; Shim, Soon‐Mi

doi:10.1039/c5fo01258a

Cited by 34 publications

(30 citation statements)

References 43 publications

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“…Besides, Graviola leaf contains various phytoconstituents such as alkaloids, flavonol triglycosides, phenolics, cyclopeptides, and essential oils [6, 13, 24]. Recently, we found that Graviola contains high concentrations of rutin, kaempferol-rutinoside, and vitamin U and effectively scavenged peroxyl and nitrogen radicals [14]. In addition, Graviola reduced the production of reactive oxygen species (ROS).…”

Section: Discussionmentioning

confidence: 99%

“…It was then stored at −20°C until use for analysis. Bioactive components in standardized GE were analyzed by LC-MS/MS as reported previously [14]. …”

Section: Methodsmentioning

confidence: 99%

“…The analytes were separated on a Zorbax Eclipse XDB-C18 (250 mm × 4.6 mm, 5 μ m, Agilent technologies) column. Detailed analytical conditions were used as described in a previous study [14]. In detail, the mobile phases used were solvent A (1% formic acid in water, HPLC grade, Sigma-Aldrich) and solvent B (acetonitrile, HPLC grade, Sigma-Aldrich) with gradient elution as follows: 95–5% A at 0–60 min.…”

Section: Methodsmentioning

confidence: 99%

“…Phenolic acids and flavonoid glycosides in Graviola leaves are known to have potent antioxidant activities [13]. Recently, we have reported that the total phenolic acid and total flavonoid contents were present in the steam and ethanol extracts of Graviola leaves in high concentrations, which is associated with their free radical-scavenging effects [14]. Additionally, the ethanol extract of Graviola leaves was found to upregulate the expression of superoxide dismutase-1 (SOD-1) and nuclear factor erythroid-2-related factor-2 (Nrf-2), which contributed to its reactive oxygen species-reducing activity.…”

Section: Introductionmentioning

confidence: 99%

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Immunomodulatory Efficacy of Standardized Annona muricata (Graviola) Leaf Extract via Activation of Mitogen‐Activated Protein Kinase Pathways in RAW 264.7 Macrophages

Kim¹,

Tran²,

Choi

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Annona muricata, commonly known as Graviola, has been utilized as a traditional medicine to treat various human diseases. The aim of this study was to examine the immune-enhancing activity of Graviola leaf extracts in RAW 264.7 macrophage cells. Active ingredients in Graviola leaf extracts (GE) were identified as kaempferol-3-O-rutinoside and quercetin-3-O-rutinoside by LC-MS/MS. When treated with steam or 50% ethanol GE, cell morphology was altered due to initiation of cell differentiation. While the cell viability was not altered by the steam GE, it was reduced by the ethanol GE. Both steam and ethanol GE induced the transcriptional expression of cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-1β, but only the steam extract upregulated inducible nitric oxide synthase (iNOS). In consistence with mRNA expression, the production of TNF-α and nitrite was elevated by both steam and ethanol extracts of Graviola leaves. This is mainly due to activation of mitogen-activated protein (MAP) kinase signaling pathways. These results suggest that Graviola leaves enhance immunity by activation of the MAP kinase pathways. These bioactive properties of Graviola indicate its potential as a health-promoting ingredient to boost the immune system.

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Section: Discussionmentioning

confidence: 99%

“…It was then stored at −20°C until use for analysis. Bioactive components in standardized GE were analyzed by LC-MS/MS as reported previously [14]. …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunomodulatory Efficacy of Standardized Annona muricata (Graviola) Leaf Extract via Activation of Mitogen‐Activated Protein Kinase Pathways in RAW 264.7 Macrophages

Kim¹,

Tran²,

Choi

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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“…The oxygen radical absorbance capacity (ORAC) assay was applied to assess the peroxy radical (ROO · ) scavenging capacity of LCL extracts according to Son et al The relative fluorescence intensity (percent) was detected every 5 min by using a microplate reader (Thermo Scientific) until the fluorescence reading was below 5% of the initial fluorescent light absorbance.…”

Section: Methodsmentioning

confidence: 99%

Changes in the profiling of bioactive components with the roasting process in Lycium chinense leaves and the anti‐obesity effect of its bioaccessible fractions

et al. 2019

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BACKGROUND: This study aimed to investigate the profiles of bioactive components in roasted Lycium chinense leaves (LCLs) and its in vitro anti-obesity activity after digestion processes. RESULTS: Chlorogenic acid, kaempferol-3-sophoroside-7-glucoside, kaempferol-3-sophoroside, and kaempferol-3-glucoside were discovered as bioactive components in various ratios of ethanol (EtOH) extract in LCLs by using ultra-performance liquid chromatography-electrospray ionization-mass spectrophotometry (UPLC-ESI-MS). The roasting process followed by a 30%EtOH extraction tended to decrease the content of chlorogenic acid and kaempferol-3-glucoside, and enhanced the content of kaempferol-3-sophoroside-7-glucoside. It effectively inhibited pancreatic lipase activity by 62.50 ± 4.81%, which was approximately 1.71 percentage points higher than that of the dried-nonroasted LCL extract (60.79 ± 3.75%). Its bioaccessible fraction obtained from in vitro digestion significantly and dose dependently reduced intracellular lipid accumulation by adipocyte 3T3-L1 compared with a 30% EtOH extraction. At a concentration of 200 g mL −1 , it inhibited lipid accumulation up to 29.55% in 3T3-L1 cells, which indicated that human digestive enzymes converted kaempferol-3-sophoroside-7-glucoside to kaempferol metabolites that have anti-obesity effects.CONCLUSION: This study suggests that the profiling of bioactive components by processing methods and a bioaccessible fraction could be crucial to improve the bioactivity of LCLs, and potentially be a natural anti-obesity ingredient after oral intake.

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Food Application and Processing: Nanotechniques and Bioactive Delivery Systems

2021

Nano‐Technological Intervention in Agricultural Productivity

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Bioefficacy of Graviola leaf extracts in scavenging free radicals and upregulating antioxidant genes

Abstract: The aims of this study were to determine bioactive components of Graviola leaf extracts and to examine the radical scavenging capacity, gene expression of antioxidant enzymes and transcription factors.

Cited by 34 publications

References 43 publications

Immunomodulatory Efficacy of Standardized Annona muricata (Graviola) Leaf Extract via Activation of Mitogen‐Activated Protein Kinase Pathways in RAW 264.7 Macrophages

Immunomodulatory Efficacy of Standardized Annona muricata (Graviola) Leaf Extract via Activation of Mitogen‐Activated Protein Kinase Pathways in RAW 264.7 Macrophages

Changes in the profiling of bioactive components with the roasting process in Lycium chinense leaves and the anti‐obesity effect of its bioaccessible fractions

Food Application and Processing: Nanotechniques and Bioactive Delivery Systems

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