Bioeffects of low dose ultrasound on neuronal cell function

Muratore, Robert; LaManna, Justine K.; Lamprecht, Michael R.; Morrison, Barclay

doi:10.1109/uia.2009.5404028

Cited by 6 publications

(6 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2): a quieting of the system at very low dose (5.5 kPa), an apparent threshold (between 20 and 48 kPa), and possible fatigue at the highest dose (77 kPa), which was the last stimulus. [10]) and Cornu Ammonis, of a rat hippocampal culture to ultrasonic stimuli. Stimulus (shown on horizontal axes) proceeded from low intensity to high intensity.…”

Section: Resultsmentioning

confidence: 99%

“…Structural changes suggest accompanying functional changes. Neuronal tissue responses to insonification have been reported peripherally [6,7] and centrally [8,9,10], although the underlying mechanism (whether radiation force directly, radiation force via extracellular and intracellular streaming, cavitation, or heating) has not yet been determined.…”

Section: Introductionmentioning

confidence: 99%

“…The rat hippocampal culture in combination with a multi-channel electrode array offers a precise means of spatially and temporally tracking responses to ultrasonic stimuli. To investigate how different hippocampal regions responded to the same stimulus, additional data from a previously reported experiment [2,10] were analyzed.…”

Section: Introductionmentioning

confidence: 99%

“…1. Full experimental details were presented previously [2,10] and are summarized here. Hippocampal slices were obtained from 8-day old Sprague Dawley rats and cultured for 6 days [2].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hippocampal culture stimulus with 4-megahertz ultrasound

Muratore¹,

LaManna²,

Lamprecht³

et al. 2012

AIP Conference Proceedings

Self Cite

View full text Add to dashboard Cite

Among current modalities, ultrasound uniquely offers both millisecond and millimeter accuracy in noninvasively stimulating brain tissue. In addition, by sweeping the ultrasound beam within the refractory period of the neuronal tissue, ultrasonic neuromodulation can be adapted to target extended or multiply connected regions with quasi-simultaneity. Towards the development of this safe brain stimulus technique, the response of rat hippocampal cultures to ultrasound was investigated. Hippocampal slices, 0.4-mm thick, were obtained from 8-day old Sprague Dawley rats and cultured for 6 days. The in vitro cultures were exposed to multiple 100-ms 4.04-MHz ultrasound pulses from a 42-mm diameter, 90-mm spherical cap transducer. Peak pressure ranged from 0 through about 77 kPa. Responses in the form of electrical potentials from a sixty channel electrode array were digitized and recorded. The DG and CA1 regions of the hippocampus exhibited similar ultrasonically-evoked field potentials.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…1. Full experimental details were presented previously [2,10] and are summarized here. Hippocampal slices were obtained from 8-day old Sprague Dawley rats and cultured for 6 days [2].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hippocampal culture stimulus with 4-megahertz ultrasound

Muratore¹,

LaManna²,

Lamprecht³

et al. 2012

AIP Conference Proceedings

Self Cite

View full text Add to dashboard Cite

show abstract

“…Guided by the mechatronics viewpoint on this ur-system, external, engineered energies (such as those available from electric, magnetic, and acoustic fields) can be injected directly into any stage of the nervous system loop. Thus it is possible to stimulate receptors in novel ways [ 2 , 3 ], or to directly stimulate an upstream stage, bypassing the sensory apparatus [ 4 , 5 ].…”

mentioning

confidence: 99%