BioE3 enables the identification ofbona fidetargets of E3 ligases
Orhi Barroso-Gomila,
Laura Merino-Cacho,
Veronica Muratore
et al.
Abstract:The post-translational modification of proteins by ubiquitination is a highly regulated process that involves a dynamic, three-step enzymatic cascade, where more than 600 E3 ligases play a critical role in recognizing specific substrates for modification. Separating bona fide targets of E3s from E3-interacting proteins remains a major challenge in the field. In this study, we present BioE3, a novel approach for identifying substrates of ubiquitin-like (UbL) E3 ligases of interest. Using BirA-E3 ligase fusion p… Show more
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