Biodiversity of phosphate solubilizing bacteria in rhizosphere of chickpea, mustard and wheat grown in different regions of Haryana

Abstract

The native population of phosphate solubilizing bacteria (PSB) was studied in the rhizosphere of chickpea, mustard and wheat grown in different regions of Haryana. A total of 193 PSB were isolated from 245 rhizospheric samples collected from south-west and north-east zones. The PSB count showed large variations (3-67 × 10 5 cfu/g) and biodiversity within the crop and place of sampling. Using biochemical analysis, the isolates were tentatively identifi ed as belonging to four genera, Pseudomonas, Aeromonas, Klebsiella and Enterobacter. Phosphate solubilization of these isolates varied from 5.9 to 123.8% and 2.2 to 227.2 μg/ ml in solid and liquid Pikovskaya's medium, respectively. Based on their morphological traits, all the isolates were placed into 20 groups, majority of them falling in the group having white, round and gummy colonies, irrespective of the crop or the region. The intrinsic antibiotic resistance pattern showed large variations among the isolates and most of the isolates were resistant to streptomycin, ampicillin and penicillin. The highest PSB number and greatest variability were found in the rhizosphere of chickpea, followed by wheat and then mustard.

Soil Properties Related to the Occurrence of Rock Phosphate-Solubilizing Bacteria in the Rhizosphere Soil of Faba Bean (Vicia faba L.) in Morocco

“…Also, similar results have been reported in Kenyan soils with a number between 0.38 and 9.1 × 10 5 CFU g −1 of soil [19] and in Chinese soils from 6 to 22 × 10 5 CFU g −1 of soil [46]. However, other studies have reported higher values than ours, such as soils under crop-pasture rotations in a no-tillage regime in Uruguay with 0.65-62 × 10 5 CFU g −1 soil [20] and 3-67 × 10 5 CFU g −1 soil in the rhizosphere of chickpea, mustard, and wheat grown in different regions of Haryana in India [47]. On the contrary, very low numbers have been reported in northern Spain with an abundance of PSB lower than 10 2 CFU g −1 soil [48], in Argentina between 0.03 and 0.08 × 10 5 CFU g −1 of soil [21], and in northern Karnataka in India from 0.01 to 0.18 × 10 5 CFU g −1 of soil [49].…”

Screening cabbage rhizosphere as a habitat for isolation of phosphate-solubilizing bacteria

“…Phosphorus can be found in nature as organic and mineral forms (Kundu et al 2009). The concentration of soluble P in soil is very low, varying from 0.001 mg L -1 in very poor soils to 1 mg L -1 in heavily fertilized soils.…”

Characterization of phosphate-solubilizing microorganisms from salt-affected soils of India and their effect on growth of sorghum plants [Sorghum bicolor (L.) Moench]

“…Many microorganisms in the soil are able to solubilize insoluble forms of Ca-bound P by excreting organic acids that either dissolve RP directly or chelate Ca 2+ ions to bring the P i into solution (Katznelson and Bose 1959), or by producing various siderophores, mineral acids, protons, humic substances, CO 2 and H 2 S. This phenomenon of solubilizing insoluble inorganic phosphates by microorganisms is known as 'mineral phosphate solubilization' (Goldstein 1986). The important genera of phosphate-solubilizing bacteria (PSB) include Achromobacter, Aerobacter, Alkaligenes, Bacillus, Pseudomonas, Serratia and Xanthomonas, Aeromonas, Klebsiella, Enterobacter (Chen et al 2006;Kundu et al 2009). In addition, certain fungi known as phosphate solubilizing fungi (PSF) have also been shown to solubilize insoluble phosphate (Rajankar et al 2007).…”