Biodiversity of Escherichia coli bacterial resistance to multidrug isolated on the Dumai coast of Indonesia

Feliatra, F.; Mardalisa, Mardalisa; Effendi, Irwan; Adelina, Adelina; Feliatra, V A

doi:10.13057/biodiv/d230102

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…This result should be a warning in considering the use of antibiotics ampicillin (penicillin) and streptomycin, as well as broad-spectrum antibiotics such as chloramphenicol, trimethoprim-sulfamethoxazole, and ampicillin, are often used as mastitis therapy in dairy cattle (Meutia et al, 2016;Haftu et al, 2012). Antibiotics Over the Counter (OTC) are thought to generate the development of E. coli resistance, so their adverse effects contribute to the problem of infection in humans (Feliatra et al, 2022). The antibiotics of tetracycline, ciprofloxacin, and sulfamethoxazoletrimethoprim showed significant sensitive results.…”

Section: Resultsmentioning

confidence: 99%

Incidence of Extended Spectrum Beta-Lactamase (ESBL) of Escherichia Coli Isolated from the Feces of Dairy Cattles in Blitar

Dameanti,

Amri,

Roosdiana

et al. 2023

RJLS

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Antibiotic resistance (AMR) has caused a serious global threat to human health, animal health and food security. The antibiotic resistance problem, especially in Gram-negative bacteria, is increasingly widespread in various areas and has increased mortality, morbidity, and incidence. This study aims to determine the incidence of ESBL E. coli and the AMR and MDR properties of E.coli isolated from the feces of dairy cattles in Blitar, which have not been reported. This research was conducted from June to September 2022 with 60 samples of dairy cattle feces in Blitar. Samples were isolated and identified until confirmed that 55 (91.6%) samples were E. coli. All samples showed AMR character with 100% streptomycin, 87.27% chloramphenicol, 85.45% ampicillin, 72.73% cefotaxime, 40% tetracycline, 21.82% ciprofloxacin, and 14.55% sulfamethoxazole-trimethoprim. Forty-nine samples were MDR, with 10.9% resistant in three groups, 34.5% in four groups, 29.1% in five groups, 10.9% in six groups, and 3.6% in seven groups. MDR E. coli isolates were then confirmed for ESBL using the DDST test. The incidence of ESBL-producing E. coli from dairy cattle feces in Blitar was 40% (22 isolates). The results of this study can be a warning in the human health, animal health and food security.

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Section: Resultsmentioning

confidence: 99%

Incidence of Extended Spectrum Beta-Lactamase (ESBL) of Escherichia Coli Isolated from the Feces of Dairy Cattles in Blitar

Dameanti,

Amri,

Roosdiana

et al. 2023

RJLS

View full text Add to dashboard Cite

show abstract

“…Antibiotic-resistant microorganisms were thought to be the result of the irrational application of antibiotics. Escherichia coli have often been used as a indicator for antibiotic resistance genes between pathogens (Feliatra et al 2022). Bacterial isolates from natural water resources carrying heavy metal-resistance genes were frequently found to be antibiotic-resistant, as both genes were present on the same plasmid (Sultan et al 2020;Siddiqui et al 2020).…”

Section: Introductionmentioning

confidence: 99%

The role of merA gene of mercury-resistant Escherichia coli from Kahayan River, Central Kalimantan, Indonesia in emerging antibiotic resistance

et al. 2023

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Abstract. Martani NS, Notobroto HB, Wasito EB, Jabal AR. 2022. The role of merA Gene of Mercury-resistant Escherichia coli from Kahayan River, Central Kalimantan, Indonesia in emerging antibiotic resistance. Biodiversitas 23: 6629-6634. This study collected nine water samples from the upstream, middle, and downstream parts of the Kahayan river, which was heavily contaminated with mercury (Hg). The water was sampled following the collection method of surface water sample (SNI 6989.57-2008). Escherichia coli has been found in the four samples out of nine samples collected. The merA gene of bacteria was identified at 1695 base pairs (bp), bla at 199 bp, tet at 494 bp, cat at 623 bp, and gyrA at 577 bp. The merA gene from S1 from the PCR results was identified at 219 bp. However, the merA gene was not amplified with a primer measuring 1695 bp. Then, sequencing was performed, and the results indicated a 48.551% homology with E. coli Tn5075 nucleotide sequences. On examination of the cat gene, all samples are positive, or target-specific bands are observed (i.e., samples 1, 5, 6, and 7). Following sequencing, 623 bp were found to have nucleotide sequences that were 100% identical to E. coli. In all samples, the gyrA gene is either positive or has a band that corresponds to the target. Meanwhile, the results of nucleotide sequence homology of 99.826% were identical to E. coli at 586 bp. No bla gene or tet gene was identified. Sample 1 plate 1, 3, 4, 5, and sample 5 plate 3 exhibited intermediate results for ampicillin antibiotics, while the remaining results are sensitive to the tested antibiotics. Therefore, the mercury-resistant merA gene found in E. coli from the Kahayan river in Central Kalimantan has not been conclusively linked to antibiotic resistance.

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Antimicrobial resistance in southeast Asian water environments: A systematic review of current evidence and future research directions

Siri

Precha

Sirikanchana

et al. 2023

Science of The Total Environment

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Biodiversity of Escherichia coli bacterial resistance to multidrug isolated on the Dumai coast of Indonesia

Cited by 3 publications

References 31 publications

Incidence of Extended Spectrum Beta-Lactamase (ESBL) of Escherichia Coli Isolated from the Feces of Dairy Cattles in Blitar

Incidence of Extended Spectrum Beta-Lactamase (ESBL) of Escherichia Coli Isolated from the Feces of Dairy Cattles in Blitar

The role of merA gene of mercury-resistant Escherichia coli from Kahayan River, Central Kalimantan, Indonesia in emerging antibiotic resistance

Antimicrobial resistance in southeast Asian water environments: A systematic review of current evidence and future research directions

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